MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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Bovine pulmonary artery endothelial cells (BPAEC) from Cell Applications, Inc. provide an excellent model system to study many aspects of cardiovascular function and disease. For example, they have been used to investigate critical signaling pathways and mechanisms relevant to proper endothelial function, such as angiogenesis, permeability and NO production. Others have employed BPAEC to search for beneficial modulators for therapeutic use, study diabetes-associated complications related to cardiovascular function, investigate mechanisms of endothelial dysfunction related to environmental pollution, smoking, oxidative stress and inflammation and search for potential treatments, and develop layered co-cultures of liver and endothelial cells that demonstrate superior liver-specific features.
BPAEC from Cell Applications, Inc. have been utilized in multiple research publications, for example to:
- Determine that induction of cellular antioxidant glutathione during moderate oxidative stress involves ARE-binding factors in a MAP kinase independent mechanism
- Show that HGF transiently increases transcription of angiotensin-converting enzyme gene via activation of Egr-1, whereas PMA regulation involves Egr-1 and additional factors
- Elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells
- Demonstrate that thiol-reactive compounds in cigarette smoke activate NADPH oxidase and increase superoxide anion production, reducing NO levels and resulting in endothelial dysfunction
- Reveal that mercury, a risk factor for cardiovascular diseases, induces PLA2 activation in endothelial cells, followed by PLD activation, and the process is mediated by thiol-redox alteration, ROS and Ca2+/calmodulin
- Demonstrate that adiponectin protects against the hyperoxia-induced endothelial barrier dysfunction and lung damage by relieving oxidative stress and normalizing thiol-redox status
- Show that TGF-β1–induced endothelial permeability involves focal adhesion and cytoskeletal rearrangement through both FAK/Src-dependent and -independent pathways
- Demonstrate that exposure to lipopolysaccharides inhibits AMPK (which is required for normal endothelial barrier function) and causes endothelial hyperpermeability and lung injury
- Demonstrate the effectiveness of myosin light chain kinase inhibitors in decreasing endothelial hyperpermeability
- Show that angiotensin II-induced apoptosis relies on activation of AMPK for ATP production, as well as for activation of SHP-2 in a signaling cascade leading to Bcl-x(L) mRNA destabilization
- Evaluate therapeutic potential of aminoguanidine in protecting endothelia from hyperglycemic complications in diabetes by blocking the reactivity of the sugar-derived dicarbonyls and preventing the formation of advanced glycation end products
- Demonstrate the roles of Egr-1, ATF-2 and Ets-1 in the regulation of angiotensin converting enzyme promoter by phorbol ester
- Develop a therapeutic peptide based on a fragment of Listeria monocytogenes internalin B that prevented angiotensin II-induced apoptosis and stimulated proliferation and cell motility by activating ERK1/2, STAT3, and phosphatidylinositol 3-kinase/Akt pathways
- Develop layered co-cultures of liver and endothelial cells that demonstrate superior liver-specific features
Normal healthy bovine pulmonary artery
No bacteria, yeast, fungi, mycoplasma
DiI-Ac-LDL uptake: Positive
Attach, spread, proliferate in Growth Med
500,000 BPAEC (2nd passage) frozen in Basal Medium w/ 10% FBS & 10% DMSO
Cryovial frozen BPAEC (B302-05), Growth Medium (B211-500), Subculture Rgnt Kit (090K)
Shipped in Gr Med, 3rd psg (flasks or plates)
At least 16
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved BPAEC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: B302K-05||Price: $736.00|
|Cryopreserved BPAEC: Frozen BPAEC (5x10^5)||Size: 1 Cryovial||CAT.#: B302-05||Price: $587.00|
|Proliferating BPAEC: Actively growing, dividing cells in medium||Size: T-25 Flask||CAT.#: B303-25||Price: $587.00|
|Proliferating BPAEC: Actively growing, dividing cells in medium||Size: T-75 Flask||CAT.#: B303-75||Price: $791.00|
|Proliferating BPAEC: Actively growing, dividing cells in medium||Size: 6 Well||CAT.#: B303-6W||Price: $737.00|
|Proliferating BPAEC: Actively growing, dividing cells in medium||Size: 96 Well||CAT.#: B303-96W||Price: $866.00|
|Bovine EC Basal Medium : Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: B210-500||Price: $57.00|
|Bovine EC Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: B211-500||Price: $105.00|
|Bovine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: B211K-500||Price: $113.00|
|Bovine EC Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 30 ml||CAT.#: B211-GS||Price: $51.00|
Extended Family Products
|BPAEC RNA : Total RNA prepared from Bovine Pulmonary Artery Endothelial Cells||Size: 10 ug||CAT.#: B302-R10||Price: $350.00|
|BPAEC RNA : Total RNA prepared from Bovine Pulmonary Artery Endothelial Cells||Size: 25 ug||CAT.#: B302-R25||Price: $700.00|
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF101K||Price: $431.00|
|Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF101KS||Price: $54.00|
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
Kondrikov, D., C. Gross, S. Black and Y. Su. 2014. Novel Peptide for Attenuation of Hyperoxia-induced Disruption of Lung Endothelial Barrier and Pulmonary Edema via Modulating Peroxynitrite Formation. J Biol Chem, 289:33355-33363.
Sherwani, S., S. Pabon, R. Patel, M. Sayyid, T. Hagele, S. Kotha, U. Magalang, K. Maddipati, and N. Parinandi. 2013. Eicosanoid Signaling and Vascular Dysfunction: Methylmercury-Induced Phospholipase D Activation in Vascular Endothelial Cells. Cell biochemistry and biophysics. 67:317-329.
Sliman, S., R. Patel, J. Cruff, S. Kotha, C. Newland, C. Schrader, S. Sherwani, T. Gurney, U. Magalang, and N. Parinandi. 2013. Adiponectin Protects Against Hyperoxic Lung Injury and Vascular Leak. Cell biochemistry and biophysics. 67:399-414.
Xing, J., Q. Wang, K. Coughlan, B. Viollet, C. Moriasi, and M.-H. Zou. 2013. Inhibition of AMP-Activated Protein Kinase Accentuates Lipopolysaccharide-Induced Lung Endothelial Barrier Dysfunction and Lung Injury in Vivo. The American journal of pathology. 182:1021-1030.
Day, R.M., Y.H. Lee, L. Han, Y.C. Kim, and Y.H. Feng. 2011. Angiotensin II activates AMPK for execution of apoptosis through energy-dependent and -independent mechanisms. American journal of physiology. Lung cellular and molecular physiology. 301:L772-781.
Matsumoto, A., H. Harada, M. Saito, and A. Taniguchi. 2011. Induction of insulin-like growth factor 2 expression in a mesenchymal cell line co-cultured with an ameloblast cell line. In Vitro Cell.Dev.Biol.-Animal. 47:675-680.
Marchenko, A.V., E.O. Stepanova, A.V. Sekridova, M.V. Sidorova, V.N. Bushuev, Z.D. Bespalova, and V.P. Shirinsky. 2010. Novel peptide inhibitors of myosin light chain kinase suppress the hyperpermeability of vascular endothelium. BIOPHYSICS. 55:926-930.
Mungunsukh, O., Y.H. Lee, A.P. Marquez, F. Cecchi, D.P. Bottaro, and R.M. Day. 2010. A tandem repeat of a fragment of Listeria monocytogenes internalin B protein induces cell survival and proliferation. American Journal of Physiology - Lung Cellular and Molecular Physiology. 299:L905-L914.
Sliman, S., T. Eubank, S. Kotha, M.L. Kuppusamy, S. Sherwani, E.C. Butler, P. Kuppusamy, S. Roy, C. Marsh, D. Stern, and N. Parinandi. 2010. Hyperglycemic oxoaldehyde, glyoxal, causes barrier dysfunction, cytoskeletal alterations, and inhibition of angiogenesis in vascular endothelial cells: aminoguanidine protection. Molecular and cellular biochemistry. 333:9-26.
Ohno, M., K. Motojima, T. Okano, and A. Taniguchi. 2009a. Induction of Drug-Metabolizing Enzymes by Phenobarbital in Layered Co-culture of a Human Liver Cell Line and Endothelial Cells. Biological and Pharmaceutical Bulletin. 32:813-817.
Ohno, M., K. Motojima, T. Okano, and A. Taniguchi. 2009b. Maturation of the Extracellular Matrix and Cell Adhesion Molecules in Layered Co-cultures of HepG2 and Endothelial Cells. Journal of biochemistry. 145:591-597.
Peltz, A., S.I. Sherwani, S.R. Kotha, J.N. Mazerik, E.S. O’Connor Butler, M.L. Kuppusamy, T. Hagele, U.J. Magalang, P. Kuppusamy, C.B. Marsh, and N.L. Parinandi. 2009. Calcium and Calmodulin Regulate Mercury-induced Phospholipase D Activation in Vascular Endothelial Cells. International Journal of Toxicology. 28:190-206.
Mungunsukh, O., A.P. Marquez, Y.H. Lee, G. Thiel, and R.M. Day. 2008. Characterization of the bovine angiotensin converting enzyme promoter: Essential roles of Egr-1, ATF-2 and Ets-1 in the regulation by phorbol ester. Gene. 421:81-88.
Ohno, M., K. Motojima, T. Okano, and A. Taniguchi. 2008. Up-regulation of drug-metabolizing enzyme genes in layered co-culture of a human liver cell line and endothelial cells. Tissue Engineering Part A. 14:1861-1869.
Hagele, T.J., J.N. Mazerik, A. Gregory, B. Kaufman, U. Magalang, M.L. Kuppusamy, C.B. Marsh, P. Kuppusamy, and N.L. Parinandi. 2007. Mercury Activates Vascular Endothelial Cell Phospholipase D through Thiols and Oxidative Stress. Int. J. Toxicol. 26:57-69.
Lee, Y.H., U.S. Kayyali, A.M. Sousa, T. Rajan, R.J. Lechleider, and R.M. Day. 2007. Transforming growth factor-β1 effects on endothelial monolayer permeability involve focal adhesion kinase/Src. American journal of respiratory cell and molecular biology. 37:485.
Mazerik, J., H. Mikkilineni., V. Kuppusamy, E. Steinhour, A. Peltz, C. Marsh, P. Kuppusamy, and N. Parinandi. 2007. Mercury Activates Phospholipase A2 and Induces Formation of Arachidonic Acid Metabolites in Vascular Endothelial Cells. Toxicol Mech and Meth, 17:541-557.
Mazerik, J.N., T. Hagele, S. Sherwani, V. Ciapala, S. Butler, M.L. Kuppusamy, M. Hunter, P. Kuppusamy, C.B. Marsh, and N.L. Parinandi. 2007. Phospholipase A2 Activation Regulates Cytotoxicity of Methylmercury in Vascular Endothelial Cells. International Journal of Toxicology. 26:553-569.
Mohammed, K.A., N. Nasreen, R.S. Tepper, and V.B. Antony. 2007. Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release. American Journal of Physiology. 292:L559-L566.
Hagele, T. 2006. Mercury activates phospholipase D in vascular endothelial cells: Implications for environmental cardiovascular disease. The Ohio State University, Honors Thesis.
Day, R.M., G. Thiel, J. Lum, R.D. Chévere, Y. Yang, J. Stevens, L. Sibert, and B.L. Fanburg. 2004. Hepatocyte Growth Factor Regulates Angiotensin Converting Enzyme Expression. Journal of Biological Chemistry. 279:8792-8801.
Ignjatovic, T., S. Stanisavljevic, V. Brovkovych, R.A. Skidgel, and E.G. Erdos. 2004. Kinin B1 Receptors Stimulate Nitric Oxide Production in Endothelial Cells: Signaling Pathways Activated by Angiotensin I-Converting Enzyme Inhibitors and Peptide Ligands. Molecular Pharmacology. 66:1310-1316.
Jaimes, E.A., E.G. DeMaster, R.-X. Tian, and L. Raij. 2004. Stable Compounds of Cigarette Smoke Induce Endothelial Superoxide Anion Production via NADPH Oxidase Activation. Arteriosclerosis, Thrombosis, and Vascular Biology. 24:1031-1036.
Chen, H., M. Montagnani, T. Funahashi, I. Shimomura, and M.J. Quon. 2003. Adiponectin Stimulates Production of Nitric Oxide in Vascular Endothelial Cells. Journal of Biological Chemistry. 278:45021-45026.