MSDS Cryopreserved Cells
Instructions BPASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Airway Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC) from Cell Applications, Inc. have been used in studies relevant to
  • Pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells, the process shown to be mediated by hypoxia-induced expression of Bcl-xl
  • Additionally, they were used to elucidate the PKC-α dependent mechanisms of proMMP-2 activation and other signaling events in smooth muscle cells under pro-inflammatory conditions related to cell growth
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Bovine Pulmonary Artery Smooth Muscle Cells, BPASMC (L) immunolabeled for smooth muscle-specific alpha-actin using ABC method (R).

Details

Tissue
Normal healthy bovine pulmonary artery
QC
No bacteria, yeast, fungi, mycoplasma 
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 BPASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen BPASMC (B352-05), Growth Medium (B311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
Can be cultured at least 16 doublings
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions BPASMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved BPASMC: Frozen BPASMC (5x10^5) Size: 1 Cryovial CAT.#: B352-05 Price: $618.84
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Cryopreserved BPASMC Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: B352K-05 Price: $767.07
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Proliferating BPASMC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: B353-25 Price: $618.84
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Proliferating BPASMC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: B353-75 Price: $823.33
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Proliferating BPASMC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: B353-6W Price: $823.33
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Proliferating BPASMC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: B353-96W Price: $952.48
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Related Products

Product Size CAT.# Price Quantity
Bovine Smooth Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: B310-500 Price: $57.04
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Bovine Smooth Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: B311-500 Price: $105.47
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Bovine Smooth Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: B311K-500 Price: $113.01
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Bovine Smooth Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml (2 parts) CAT.#: B311-GS Price: $57.04
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Bovine Smooth Muscle Cell Induction Medium: Provides signals that change cell behavior Size: 250 ml CAT.#: B311I-250 Price: $62.42
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.21
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $53.76
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Airway Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2016
Chowdhury, A., J. Sarkar, P. Pramanik, T. Chakraborti, S. and S. Chakraborti. 2016. Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation. Archives of Biochemistry and Biophysics, 603:91–101.
Chowdhury, A., S. Nandy, J. Sarkar, T. Chakraborti and S. Chakraborti. 2011. Inhibition of pro-/active MMP-2 by green tea catechins and prediction of their interaction by molecular docking studies. Molec Cell Biochem, DOI 10.1007/s11010-016-2903-y.
Sarkar, J., S. Nandy, A. Chowdhury, T. Chakraborti and S. Chakraborti. 2016. Inhibition of MMP-9 by green tea catechins and prediction of their interaction by molecular docking analysis. Biomed & Pharmacother, 84:340-347.
Sarkar, J., A. Chowdhury, T. Chakraborti and S. Chakraborti. 2016. Cross-talk between NADPH oxidase-PKCα-p38MAPK and NF-κB-MT1MMP in activating proMMP-2 by ET-1 in pulmonary artery smooth muscle cells. Molec & Cell Biochm, 415:13-28.
2015
Chakraborti, S., A. Chowdhury and T. Chakraborti. 2015. Cross-talk between p38MAPK and Giα in regulating cPLA2 activity by ET-1 in pulmonary smooth muscle cells. Mol Cell Biochem, 400: 107.
2014
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey and S. Chakraborti. 2014. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molec & Cell Biochem, 385:53-68.
Rahaman, S., K. Dey, P. Das, S. Roy, T. Chakraborti and S. Chakraborti. 2014. Identification, purification and partial characterization of low molecular weight protein inhibitor of Na+/K+-ATPase from pulmonary artery smooth muscle cells. Molec & Cellular Biol, 393:309-317.
2013
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey, and S. Chakraborti. 2013. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molecular and cellular biochemistry:1-16.
Roy, S., T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2013. Role of PKC-α in NF-κB–MT1-MMP–mediated activation of proMMP-2 by TNF-α in pulmonary artery smooth muscle cells. Journal of biochemistry. 153:289-302.
Shaikh, S., J. Sarkar, A. Pramanik, K. Karmakar, and S. Chakraborti. 2013. Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain. IJBB. 50:419-427.
2012
Dey, K., S. Roy, B. Ghosh, and S. Chakraborti. 2012. Role of protein kinase C in phospholemman mediated regulation of α2β1 isozyme of Na+K+ ATPase in caveolae of pulmonary artery smooth muscle cells. Biochimie. 94:991-1000.
2011
Roy, S., K. Samanta, T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2011. Role of TGF-β1 and TNF-α in IL-1β mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: Involvement of an aprotinin sensitive protease. Archives of biochemistry and biophysics. 513:61-69.
2009
Chakraborti, S., A. Chowdhury, P. Kar, P. Das, S. Shaikh, S. Roy, and T. Chakraborti. 2009. Role of protein kinase C in NADPH oxidase derived O2(*-)-mediated regulation of Kv–LVOCC axis under U46619 induced increase in [Ca2+] in pulmonary smooth muscle cells. Archives of biochemistry and biophysics. 487:123-130.
2007
Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.