MSDS Cryopreserved Cells
Instructions CnAOEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Canine Aortic Endothelial Cells (CnAOEC) from Cell Applications, Inc. provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health.
 
CnAOEC have been used in numerous research publications, for instance to:
 
  • Adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs
  • Investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs
  • Demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent cardiovascular disease-related endothelial disfunction in dogs
  • Compare growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis
  • Understand reasons for reduced numbers of circulating progenitor cells in cardiovascular disease
(Click to Enlarge) Canine Aortic Endothelial Cells: CnAOEC

Details

Tissue
Normal healthy Canine aorta
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
BioassayAttach, spread, proliferate in Growth Med
Cryovial
500,000 cells (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen CnAOEC (Cn304-05), Growth Medium (Cn211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions CnAOEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved CnAOEC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: Cn304K-05 Price: $849.00
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Cryopreserved CnAOEC: Frozen CnAOEC (5x10^5) Size: 1 Cryovial CAT.#: Cn304-05 Price: $694.00
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Proliferating CnAOEC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: Cn305-25 Price: $694.00
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Proliferating CnAOEC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: Cn305-75 Price: $899.00
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Proliferating CnAOEC: Actively growing, dividing cells in medium Size: 24 Well CAT.#: Cn305-24W Price: $899.00
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Proliferating CnAOEC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: Cn305-96W Price: $1,028.00
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Related Products

Product Size CAT.# Price Quantity
Canine EC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: Cn210-500 Price: $59.00
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Canine EC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: Cn211-500 Price: $110.00
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Canine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: Cn211K-500 Price: $118.00
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Canine EC Growth Medium wo Hydrocortisone: All-in-one ready-to-use, without Hydrocortisone Size: 500 CAT.#: CN211H-500 Price: $127.00
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Canine EC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: Cn211-GS Price: $59.00
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Extended Family Products

Product Size CAT.# Price Quantity
CnAOEC RNA: Total RNA prepared from Canine Aortic Endothelial Cells Size: 10 ug CAT.#: Cn304-R10 Price: $317.00
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CnAOEC RNA: Total RNA prepared from Canine Aortic Endothelial Cells Size: 25 ug CAT.#: Cn304-R25 Price: $635.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

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Publications

2107

Witter, L, E. Gruber, F. Lean and T. Stokol. 2017. Evaluation of procoagulant tissue factor expression in canine hemangiosarcoma cell lines. Am J Vet Res, 78:69-79.

2016
Anwar, S., T. Yanai, and H. Sakai. 2016. Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma. J Comparative Pathol, 155:29-39.
Roshan, A., L. Dong, C. Alvan, J. Bart and H. Wenhu. 2016. Validation of Cross-Species Reactivity of the VEGF-A/PDGFRβ Bifunctional Antibody PF-06653157J Ocular Pharmacol & Ther, 32:650-658.
2015
González-Miguel, J., R. Morchón, E. Carretón, J. Montoya-Alonso and F. Simón. 2015. Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis? Parasites & Vectors, 8:194.
González-Miguel J, R. Morchón , M. Siles-Lucas, and F. Simón F. 2015. Fibrinolysis and Proliferative Endarteritis: Two Related Processes in Chronic Infections? The Model of the Blood-Borne Pathogen Dirofilaria immitis. PLoS ONE 10(4): e0124445.
González-Miguela, J., R. Morchóna, M. Siles-Lucasb, A. Oleagab, and F. Simóna. 2015. Surface-displayed glyceraldehyde 3-phosphate dehydrogenase and galectin from Dirofilaria immitis enhance the activation of the fibrinolytic system of the host. Acta Tropica, 145:8–16.
Heishima, K., T. Mori, Y. Ichikawa, H. Sakai, Y. Kuranaga, T. Nakagawa, Y. Tanaka, Y. Okamura, M. Masuzawa, N. Sugito, M. Murakami, N. Yamada, Y. Akao, and K. Maruo. 2015. MicroRNA-214 and MicroRNA-126 Are Potential Biomarkers for Malignant Endothelial Proliferative Diseases. Int J Mol Sci, 16:25377-25391.
Heishima, K. T. Mori, H. Sakai, N. Sugito, M. Murakami, N. Yamada, Y. Akao, and K. Maruo. 2015. MicroRNA-214 Promotes Apoptosis in Canine Hemangiosarcoma by Targeting the COP1-p53 Axis. PLOS One, DOI: 10.1371/journal.pone.0137361.
Swancutt, K. 2015. Single high dose irradiation: Applicability of cell survival curve modeling and in vivo evaluation of tumor biologic response. PhD Dissertation, Colorado State University.
2013
Osterbur, K., D.-H. Yu, and A.E. DeClue. 2013. Interleukin-1β, tumour necrosis factor-α and lipopolysaccharide induce C-type natriuretic peptide from canine aortic endothelial cells. Research in Veterinary Science. 94:478-483.
2012
Baumgartner-Parzer, S.M., F.R. Waldenberger, A. Freudenthaler, A. Ginouvès-Guerdoux, D. McGahie, and H. Gatto. 2012. The Natural Antioxidants, Pomegranate Extract and Soy Isoflavones, Favourably Modulate Canine Endothelial Cell Function. ISRN veterinary science. 2012: doi:590310.595402/592012/590328.
Monginoux, P. H. Gatto, C. Karst, and F. Waldenberger. 2012. Products for oral administration comprising extracts of punica granatum (pomegranate), intended for a pet, and applications of same. Patent Application US 20140335180 A1.
Murai, A., S. Asa, A. Kodama, A. Hirata, T. Yanai, and H. Sakai. 2012. Constitutive phosphorylation of the mTORC2/Akt/4E-BP1 pathway in newly derived canine hemangiosarcoma cell lines. BMC Veterinary Research. 8:128.
Osterbur, K. 2012. The mechanism of C-type natriuretic peptide production in dogs and its use as a prognostic indicator in critically ill dogs. University of Missouri, MSc dissertation.
2010
Boilson, B.A., K. Larsen, A. Harbuzariu, S. Delacroix, J. Korinek, H. Froehlich, K.R. Bailey, C.G. Scott, B.P. Shapiro, G. Boerrigter, H.H. Chen, M.M. Redfield, J.C. Burnett, Jr., and R.D. Simari. 2010. Regulation of circulating progenitor cells in left ventricular dysfunction. Circulation. Heart failure. 3:635-642.
2009
Wills, T.B., A.M. Heaney, K. Jane Wardrop, and G.J. Haldorson. 2009. Immunomagnetic isolation of canine circulating endothelial and endothelial progenitor cells. Veterinary Clinical Pathology. 38:437-442.