MSDS Cryopreserved Cells
Instructions CnAOSMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Canine Aortic Smooth Muscle Cells (CnAOSMC) are derived from tunica intima and tunica media of normal canine aorta. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.
A sampling of studies using CnAOSMC concluded the following:
  • Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in intimal hyperplasia and atherosclerosis
  • Significant regional differences in PDGF production exists in normal canine aorta, and that SMC are a significant contributor to the regional variation in PDGF production
  • Nebivolol relaxes vascular smooth muscle by NO- and cyclic GMP-dependent mechanisms
  • Graft SMCs are functionally altered producing more platelet-derived growth factor (PDGF) than aortic SMCs. PDGF produced by graft SMCs may contribute to the development of intimal hyperplasia. Graft SMCs have decreased proliferation response, but have similar migratory response to PDGF compared with aortic SMCs
  • Intrinsic differences in endothelial cells from proximal aorta versus the distal aorta have different capacity to produce PDGF in response to stimulants whereas unstimulated SMCs do not exhibit regional variation in PDGF production and did not increase PDGF secretion after PMA or thrombin treatment
  • Identification of the cDNA for canine isoform of nitric oxide synthase (iNOS) can be used in the study of allograft rejection and cardiovascular disease
(Click to Enlarge) Canine Aortic Smooth Muscle Cells: CnAOSMC.  Isolated from the main vessel supplying oxygenated blood to the circulatory system.

Details

Tissue
Normal healthy canine aorta
QC
No bacteria, yeast, fungi, mycoplasma
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
2500,000 CnAOSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen CnAOSMC (Cn354-05), Gr Med (Cn311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions CnAOSMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved CnAOSMC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: Cn354K-05 Price: $838.00
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Cryopreserved CnAOSMC: Frozen CnAOSMC (5x10^5) Size: 1 Cryovial CAT.#: Cn354-05 Price: $694.00
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Proliferating CnAOSMC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: Cn355-25 Price: $694.00
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Proliferating CnAOSMC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: Cn355-75 Price: $899.00
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Proliferating CnAOSMC: Actively growing, dividing cells in medium Size: 24 Well CAT.#: Cn355-24W Price: $899.00
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Proliferating CnAOSMC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: Cn355-96W Price: $1,028.00
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Related Products

Product Size CAT.# Price Quantity
Canine SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: Cn310-500 Price: $57.00
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Canine SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: Cn311-500 Price: $99.00
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Canine SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: Cn311K-500 Price: $108.00
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Canine SMC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: Cn311-GS Price: $51.00
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Canine SMC Induction Medium: Provides signals that change cell behavior Size: 250 ml CAT.#: Cn311I-250 Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2016
2015
González-Miguel J, R. Morchón , M. Siles-Lucas, and F. Simón F. 2015. Fibrinolysis and Proliferative Endarteritis: Two Related Processes in Chronic Infections? The Model of the Blood-Borne Pathogen Dirofilaria immitis. PLoS ONE 10(4): e0124445.