MSDS Cryopreserved Cells
Instructions CnAOSMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Canine Aortic Smooth Muscle Cells (CnAOSMC) are derived from tunica intima and tunica media of normal canine aorta. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.
A sampling of studies using CnAOSMC concluded the following:
- Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in intimal hyperplasia and atherosclerosis
- Significant regional differences in PDGF production exists in normal canine aorta, and that SMC are a significant contributor to the regional variation in PDGF production
- Nebivolol relaxes vascular smooth muscle by NO- and cyclic GMP-dependent mechanisms
- Graft SMCs are functionally altered producing more platelet-derived growth factor (PDGF) than aortic SMCs. PDGF produced by graft SMCs may contribute to the development of intimal hyperplasia. Graft SMCs have decreased proliferation response, but have similar migratory response to PDGF compared with aortic SMCs
- Intrinsic differences in endothelial cells from proximal aorta versus the distal aorta have different capacity to produce PDGF in response to stimulants whereas unstimulated SMCs do not exhibit regional variation in PDGF production and did not increase PDGF secretion after PMA or thrombin treatment
- Identification of the cDNA for canine isoform of nitric oxide synthase (iNOS) can be used in the study of allograft rejection and cardiovascular disease
(Click to Enlarge) Canine Aortic Smooth Muscle Cells: CnAOSMC. Isolated from the main vessel supplying oxygenated blood to the circulatory system.
Details
Tissue | Normal healthy canine aorta |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 2500,000 CnAOSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen CnAOSMC (Cn354-05), Gr Med (Cn311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Canine Aortic Smooth Muscle Cell Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: Cn354K-05 | Price: $912.00 | |
| Cryopreserved Canine Aortic Smooth Muscle Cells (CnAOSMC): Frozen CnAOSMC (5x10^5) | Size: 1 Cryovial | CAT.#: Cn354-05 | Price: $745.00 | |
| Proliferating Canine Aortic Smooth Muscle Cells (CnAOSMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: Cn355-25 | Price: $745.00 | |
| Proliferating Canine Aortic Smooth Muscle Cells (CnAOSMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: Cn355-75 | Price: $935.00 | |
| Proliferating Canine Aortic Smooth Muscle Cells (CnAOSMC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: Cn355-24W | Price: $935.00 | |
| Proliferating Canine Aortic Smooth Muscle Cells (CnAOSMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: Cn355-96W | Price: $1,055.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Canine SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: Cn310-500 | Price: $74.00 | |
| Canine SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: Cn311-500 | Price: $114.00 | |
| Canine SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: Cn311K-500 | Price: $124.00 | |
| Canine SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: Cn311-GS | Price: $66.00 | |
| Canine SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: Cn311I-250 | Price: $78.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2016
González-Miguel, J., C. Larrazabal, D. Loa-Mesón, M. Siles-Lucas, F. Simón and R. Morchón. 2016. Glyceraldehyde 3-phosphate dehydrogenase and galectin from Dirofilaria immitis participate in heartworm disease endarteritis via plasminogen/plasmin system. Veterinary Parasitology, 223:96-101.
2015
González-Miguel J, R. Morchón , M. Siles-Lucas, and F. Simón F. 2015. Fibrinolysis and Proliferative Endarteritis: Two Related Processes in Chronic Infections? The Model of the Blood-Borne Pathogen Dirofilaria immitis. PLoS ONE 10(4): e0124445.