5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.
Canine Chondrocytes (CnC) from Cell Applications, Inc. were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III and trans-pterostilbene. They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices.
Normal healthy canine articular cartilage
No bacteria, yeast, fungi, mycoplasma
Attach, spread, proliferate in Growth Med
500,000 CnC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Cryovial frozen CnC (Cn402-05), Growth Medium (Cn411-500), Subcltr Rgnt Kit (090K)
Shipped in Gr Med, 2nd psg (flasks or plates)
At least 10
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved CnC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: Cn402K-05||Price: $841.00|
|Cryopreserved CnC: Frozen CnC (5x10^5)||Size: 1 Cryovial||CAT.#: Cn402-05||Price: $694.00|
|Proliferating CnC: Actively growing, dividing cells, in medium||Size: T-25 Flask||CAT.#: Cn403-25||Price: $694.00|
|Proliferating CnC: Actively growing, dividing cells, in medium||Size: T-75 Flask||CAT.#: Cn403-75||Price: $899.00|
|Proliferating CnC: Actively growing, dividing cells, in medium||Size: 6 well||CAT.#: Cn403-6W||Price: $899.00|
|Proliferating CnC: Actively growing, dividing cells, in medium||Size: 96 Well||CAT.#: Cn403-96W||Price: $1,028.00|
|CnC Basal Medium: Without growth supplement (GS). Add GS before use.||Size: 500 ml||CAT.#: Cn410-500||Price: $57.00|
|CnC Differentiation Medium: Promotes cells to change from one type to another, more specialized||Size: 250 ml||CAT.#: Cn411D-250||Price: $71.00|
|CnC Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: Cn411-500||Price: $98.00|
|CnC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: Cn411K-500||Price: $113.00|
|CnC Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 50 ml||CAT.#: Cn411-GS||Price: $51.00|
Extended Family Products
|CnC RNA : Total RNA prepared from Canine Chondrocytes||Size: 10 ug||CAT.#: Cn402RD-R10||Price: $350.00|
|CnC RNA : Total RNA prepared from Canine Chondrocytes||Size: 25 ug||CAT.#: Cn402RD-R25||Price: $700.00|
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF402K||Price: $431.00|
|Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF402KS||Price: $54.00|
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
Martinez, S., Y. Chen, E. Ho, S. Martinez, and N. Davies. 2015. Pharmacological effects of a C-phycocyanin-based multicomponent nutraceutical in an in-vitro canine chondrocyte model of osteoarthritis. Canadian J Vet Res, 79:241-249.
Yamka, R. and N. Frantz. 2015. Compositions including pyruvate for companion animals and methods of use thereof. Patent US 8999375 B2.
Shaik, J., J.S. Mohammed, M. McShane, and D. Mills. 2013a. In vitro evaluation of chondrosarcoma cells and canine chondrocytes on layer-by-layer (LbL) self-assembled multilayer nanofilms. Biofabrication. 5:015004.
Shaik, J., J. Shaikh Mohammed, M.J. McShane, and D.K. Mills. 2013b. Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off: Morphological Analysis. Journal of Medical Engineering. 2013.
Wright, L.D., K.D. McKeon-Fischer, Z. Cui, L.S. Nair, and J.W. Freeman. 2012. PDLA/PLLA and PDLA/PCL nanofibers with a chitosan-based hydrogel in composite scaffolds for tissue engineered cartilage. Journal of tissue engineering and regenerative medicine: 10.1002/term.1591
Yamka, R. and N. Frantz. 2010. Compositions including pyruvate for companion animals and methods of use thereof. Patent Application US 20120289598 A1.
Remsberg, C.M., J.A. Yáñez, Y. Ohgami, K.R. Vega-Villa, A.M. Rimando, and N.M. Davies. 2008. Pharmacometrics of pterostilbene: preclinical pharmacokinetics and metabolism, anticancer, antiinflammatory, antioxidant and analgesic activity. Phytotherapy Research. 22:169-179.
Yáñez, J.A., C.M. Remsberg, K.R. Vega-Villa, N.D. Miranda, J. Navas, Y. Ohgami, D. McCormick, K. Hughes, C. Temple, and S.A. Martinez. 2008. Pharmacological evaluation of Glyco-Flex® III and its constituents on canine chondrocytes. Journal of Medical Sciences. 8:1-14.