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Instructions CnTMC
Description
Canine Trabecular Meshwork Cells (CnTMC) are primary cells derived from canine eyes. CnTMC are cultured in Canine Trabecular Meshwork Cell Growth Medium and are able to propagate to 10 population doublings when cultured under the recommended conditions. Trabecular Meshwork cells are endothelial-like cells in a sponge-like connective tissue located near the front of the eye and are responsible for regualting eye pressure by controlling drainage of fluid into tubes that flow into the bloodstream. Outflow is mediated by alterations in contractility and tension of TM, which also serve as a self-cleaning filter due to their phagocytic nature. Live cell imaging of the cytoskeleton provides valuable information on actin dynamics in CnTMC. Other research aims to identify treatments that relax TM contraction to increase fluid outflow and lower eye pressure. Specific targeting of TM could also play a clinical role by increasing therapeutic efficacy of nanoparticles for gene delivery. Damage and dysfunction of TM have clinical significance. For instance, hypoxia increases DNA methylation, accompanied by altered gene expression, whereas during the normal aging process, TM number decreases, and senescent cells accumulate.
In Glaucoma, a leading cause of irreversible blindness, decreased fluid outflow causes an elevation of intraocular pressure and progressive loss of retinal ganglion cells. Physical changes to the TM include increased fibrosis, fibronectin accumulation, and expression of ECM cross-linking enzymes. This cytoskeletal reorganization and cell loss causes the TM to become rigid and stiff. Other glaucoma-related dysfunctions include mitochondrial defects, altered signaling pathways, elevated TGF-β2, genomic DNA defects, and oxidation damage.
Canine Trabecular Meshwork Cell: CnTMC Top: Phase-contrast, Bottom Left: CnTMC immunostained for alpha-Smooth Muscle Actin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.
Details
| Tissue | Normal healthy canine cornea |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 CnTMC frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen CnTMC (Cn634-05), Growth Medium (Cn631-500), Subculture Rgnt Kit (090K) |
| Proliferating | Shipped in Tsfr Med, flasks or plates |
| Doublings | At least 12 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Proliferating Canine Trebecular Meshwork Cells (CnTMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: Cn635-25 | Price: $886.00 | |
| Proliferating Canine Trebecular Meshwork Cells (CnTMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: Cn635-75 | Price: $1,076.00 | |
| Proliferating Canine Trebecular Meshwork Cells (CnTMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: Cn635-96W | Price: $1,196.00 | |
| Cryopreserved Canine Trebecular Meshwork Cells (CnTMC): Frozen CnTMC (5x10^5) | Size: 1 Cryovial | CAT.#: Cn634-05 | Price: $886.00 | |
| Cryopreserved Canine Trabecular Meshwork Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: Cn634K-05 | Price: $1,060.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| CnTMC Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: Cn630-500 | Price: $85.00 | |
| CnTMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: Cn631-500 | Price: $121.00 | |
| CnTMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: 500 ml | CAT.#: Cn631K-500 | Price: $132.00 | |
| CnTMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: Cn631K-GS | Price: $65.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Human Fibronectin ELISA Kit: Human Fibronectin ELISA Kit | Size: 96 Wells | CAT.#: CL0349 | Price: $457.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |