Product Sheet RP1044
Product Sheet RP1044AF
Description
BACKGROUND Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%.
Recombinant human VEGF 165 is a non-glycosylated homodimer, containing two 165 amino acids and having a total molecular mass of 38.2 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP1044 / RP1044AF
Alternative Name:
VEGF-A, VPF, glioma-derived endothelial cell mitogen
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human VEGF 165 is lyophilized with no additives.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) and is typically 1-6 ng/mL.
AA Sequence:
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Vascular Endothelial Growth Factor-165 (VEGF-165): Human Vascular Endothelial Growth Factor-165 | Size: 10 ug | CAT.#: RP1044-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-165 (VEGF-165): Human Vascular Endothelial Growth Factor-165 | Size: 100 ug | CAT.#: RP1044-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-165 (VEGF-165): Human Vascular Endothelial Growth Factor-165 | Size: 1000 ug | CAT.#: RP1044-1000 | Price: $4,090.00 | |
| Human VEGF-165, Animal-Free: Human Vascular Endothelial Growth Factor-165, Animal-Free | Size: 10 ug | CAT.#: RP1044AF-10 | Price: $277.00 | |
| Human VEGF-165, Animal-Free: Human Vascular Endothelial Growth Factor-165, Animal-Free | Size: 100 ug | CAT.#: RP1044AF-100 | Price: $626.00 | |
| Human VEGF-165, Animal-Free: Human Vascular Endothelial Growth Factor-165, Animal-Free | Size: 1000 ug | CAT.#: RP1044AF-1000 | Price: $5,286.00 |