Instructions TF402K
Instructions TF402KS

Description

Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. Cytofect™ Chondrocyte Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into chondrocytes. This kit contains Cytofect-2, Viral Enhancer* and Media for transfection and culturing cells. Viral Enhancer complexes with DNA and Cytofect-2 and escorts the tranfection complex to the nucleus. This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary cells.
#Wells / Rxns
96 / 875
24 / 175
12 / 86
The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of Human Chondrocytes with good efficiency (35%) and viability (80%-95%).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. Viral Enhancer (VE) is a replication-deficient, Adenovirus-derived formulation that does not contain replication competent virus. TR1 and VE are manufactured by Targeting Systems.

Transfection of adult HC (Cat. No. 402-05a) with a GFP-expression plasmid using CF2 with VE*.

Details

Kit Contents:Cytofect -2
Viral Enhancer (VE)
Antibiotics-Free Growth Medium
Transfection Medium
 
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF402K

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Instructions TF402KS

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $430.50
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Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $53.76
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Related Products

Product Size CAT.# Price Quantity
Cytofect-1 (CF1): Store at -20°C Size: 250 ul CAT.#: 051-250 Price: $151.70
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Cytofect-1 (CF1): Store at -20°C Size: 500 ul CAT.#: 051-500 Price: $246.00
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Cytofect-2 (CF2): Store at 4°C Size: 500 ul CAT.#: 052-500 Price: $246.00
-+
Cytofect-2 (CF2): Store at 4°C Size: 250 ul CAT.#: 052-250 Price: $151.70
-+
Peptide Enhancer (PE): Store at 4°C Size: 250 ul CAT.#: 053-250 Price: $123.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 500 ul CAT.#: 053-500 Price: $205.00
-+
Viral Enhancer (VE): Store at -20°C Size: 250 ul CAT.#: 054-250 Price: $123.00
-+
Viral Enhancer (VE): Store at -20°C Size: 500 ul CAT.#: 054-500 Price: $205.00
-+

Resources/Documents

Publications

2016
Sahoo, S., D. Meijles, I. Ghouleh, M. Tandon, E. Cifuentes-Pagano, J. Sembrat, M. Rojas, E. Goncharova and P. Pagano. 2016. MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension. PLoS ONE, dx.doi.org/10.1371/journal.pone.0153780.
2015
Dokun, A., L. Chen, M. Okutsu, C. Farber, S. Hazarika, W. Jones, D. Craig, D. Marchuk, J. Lye, S. Shan, and B. Annex. 2015. ADAM12: A Genetic Modifier of Pre-clinical Peripheral Arterial Disease. Am J Physiol, DOI: 10.1152/ajpheart.00803.2014.
Zelko, I., and R. Folz. 2015. Regulation of Oxidative Stress in Pulmonary Artery Endothelium: Modulation of EC-SOD and NOX4 Expression Using HDAC Class I Inhibitors. Am J Respir Cell Mol Biol, DOI: 10.1165/rcmb.2014-0260OC.
2013
Chen, Z., T.C. Lai, Y.H. Jan, F.M. Lin, W.C. Wang, H. Xiao, Y.T. Wang, W. Sun, X. Cui, Y.S. Li, T. Fang, H. Zhao, C. Padmanabhan, R. Sun, D.L. Wang, H. Jin, G.Y. Chau, H.D. Huang, M. Hsiao, and J.Y.J. Shyy. 2013. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. The Journal of Clinical Investigation. 123:1057-1067.
He, M., X. Liang, L. He, W. Wen, S. Zhao, L. Wen, Y. Liu, J.Y.J. Shyy, and Z. Yuan. 2013. Endothelial dysfunction in rheumatoid arthritis: the role of monocyte chemotactic protein-1-induced protein. Arteriosclerosis, Thrombosis, and Vascular Biology. 33:1384-1391.
Yan, Y., X. Li, K. Kover, M. Clements, and P. Ye. 2013. CREB participates in the IGFIstimulation cyclin D1 transcription. Developmental Neurobiology. 73:559-570.
2012
Jeon, Y.J., M.G. Jo, H.M. Yoo, S.H. Hong, J.M. Park, S.H. Ka, K.H. Oh, J.H. Seol, Y.K. Jung, and C.H. Chung. 2012. Chemosensitivity is controlled by p63 modification with ubiquitin-like protein ISG15. The Journal of Clinical Investigation. 122:2622-2636.