Instructions TF350K
Instructions TF350KS

Description

Transfection, the introduction of nucleic acid into mammalian cells, is a powerful technique used to study in vivo gene function and regulation.  The Cytofect™ Smooth Muscle Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of smooth muscle cells. This kit contains Cytofect-2 and Media for transfection and culturing cells.  The result has been high transfection efficiency and viability for these traditionally hard-to-transfect cells.
#Wells / Rxns
96 / 500
24 / 125
12 / 62
Cytofect™ Smooth Muscle Cell Transfection Kit contains all the necessary reagents and media for transfection. Optimized transfection protocols are provided with the kit to carry out successful transfection of the following smooth muscle cells with high efficiency (40%-90%) and viability (80%-95%):
Products are for research use only. They are not intended for human, animal, or diagnostic applications. CF1 is manufactured by Targeting Systems.
(Click to Enlarge) Transfection of HCASMC with a GFP-expression plasmid using the Cytofect™ Smooth Muscle Cell Transfection Kit.

Details

Kit Contents:
Cytofect -2
Antibiotics-Free Growth Medium
Transfection Medium
 
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF350K

Format: PDF

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Instructions TF350KS

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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Related Products

Product Size CAT.# Price Quantity
Cytofect-1 (CF1): Store at -20°C Size: 250 ul CAT.#: 051-250 Price: $152.00
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Cytofect-1 (CF1): Store at -20°C Size: 500 ul CAT.#: 051-500 Price: $246.00
-+
Cytofect-2 (CF2) : Store at 4°C Size: 250 ul CAT.#: 052-250 Price: $152.00
-+
Cytofect-2 (CF2) : Store at 4°C Size: 500 ul CAT.#: 052-500 Price: $246.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 500 ul CAT.#: 053-500 Price: $205.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 250 ul CAT.#: 053-250 Price: $123.00
-+
Viral Enhancer (VE): Store at -20°C Size: 250 ul CAT.#: 054-250 Price: $123.00
-+
Viral Enhancer (VE): Store at -20°C Size: 500 ul CAT.#: 054-500 Price: $205.00
-+

Resources/Documents

Publications

2016
Sahoo, S., D. Meijles, I. Ghouleh, M. Tandon, E. Cifuentes-Pagano, J. Sembrat, M. Rojas, E. Goncharova and P. Pagano. 2016. MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension. PLoS ONE, dx.doi.org/10.1371/journal.pone.0153780.
2015
Dokun, A., L. Chen, M. Okutsu, C. Farber, S. Hazarika, W. Jones, D. Craig, D. Marchuk, J. Lye, S. Shan, and B. Annex. 2015. ADAM12: A Genetic Modifier of Pre-clinical Peripheral Arterial Disease. Am J Physiol, DOI: 10.1152/ajpheart.00803.2014.
Zelko, I., and R. Folz. 2015. Regulation of Oxidative Stress in Pulmonary Artery Endothelium: Modulation of EC-SOD and NOX4 Expression Using HDAC Class I Inhibitors. Am J Respir Cell Mol Biol, DOI: 10.1165/rcmb.2014-0260OC.
2013
Chen, Z., T.C. Lai, Y.H. Jan, F.M. Lin, W.C. Wang, H. Xiao, Y.T. Wang, W. Sun, X. Cui, Y.S. Li, T. Fang, H. Zhao, C. Padmanabhan, R. Sun, D.L. Wang, H. Jin, G.Y. Chau, H.D. Huang, M. Hsiao, and J.Y.J. Shyy. 2013. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. The Journal of Clinical Investigation. 123:1057-1067.
He, M., X. Liang, L. He, W. Wen, S. Zhao, L. Wen, Y. Liu, J.Y.J. Shyy, and Z. Yuan. 2013. Endothelial dysfunction in rheumatoid arthritis: the role of monocyte chemotactic protein-1-induced protein. Arteriosclerosis, Thrombosis, and Vascular Biology. 33:1384-1391.
Yan, Y., X. Li, K. Kover, M. Clements, and P. Ye. 2013. CREB participates in the IGFIstimulation cyclin D1 transcription. Developmental Neurobiology. 73:559-570.
2012
Jeon, Y.J., M.G. Jo, H.M. Yoo, S.H. Hong, J.M. Park, S.H. Ka, K.H. Oh, J.H. Seol, Y.K. Jung, and C.H. Chung. 2012. Chemosensitivity is controlled by p63 modification with ubiquitin-like protein ISG15. The Journal of Clinical Investigation. 122:2622-2636.