Product Sheet RP3027
Description
BACKGROUND Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. FGF-9 is an autocrine/paracrine growth factor considered to be important for the growth and survival of motorneurons and prostate tissue.
Recombinant rat FGF-9 is a non-glycosylated protein, containing 205 amino acids and having a molecular mass of 23.3 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP3027
Alternative Name:
Glial activating factor, GAF, Heparin-binding growth factor-9, HBGF-9
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant rat FGF-9 is lyophilized from a sterile solution (1mg/ml) containing 10mM sodium phosphophate buffer, pH 7.5 and 75mM (NH4)2SO4.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors and is typically less than 0.5 ng/ml.
AA Sequence:
PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 10 ug | CAT.#: RP3027-10 | Price: $194.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 100 ug | CAT.#: RP3027-100 | Price: $484.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 1000 ug | CAT.#: RP3027-1000 | Price: $4,090.00 |