MSDS Cryopreserved Cells
Instructions HAOEC Normal
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Human Aortic Endothelial Cells (HAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications to study diabetes-associated complications related to cardiovascular function, investigate mechanisms of immune response and graft rejection, study endothelial dysfunction caused by air pollution, oxidative stress and inflammation, and develop 3d endothelialized engineered tissues, as well as new technologies based on novel material surfaces and drugs in order to reduce risks associated with vascular implants.
Select HAOEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx). More information about pre-screened endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
HAOEC from Cell Applications, Inc. have been used to:
- Demonstrate that increased glucose flux leads to endothelial dysfunction in diabetes via activating Egr1-mediated proinflammatory and prothrombotic responses
- Study apoptosis, oxidative stress and inflammation associated with atherosclerosis and demonstrate the beneficial effects of anthocyanin on endothelial cells damaged by exposure to oxidized sterols
- Demonstrate that upregulation of thioredoxin via AMPK-FOXO3 pathway protects endothelial cells from oxidative stress and may prevent cardiovascular diseases in patients with metabolic syndrome and diabetes and further elucidate the involvement of AMPK cascade in mediating beneficial cardiovascular effects of green tea
- Test anti-inflammatory and vasodilating properties of a synthetic rutaecarpine derivative
- Show that glycated albumin, associated with diabetic complications, decreases endothelial miR-146a expression which leads to increased IL-6 production, and that angiotensin protects endothelial cells by preventing miR-146a downregulation
- Demonstrate that air pollutants can directly affect ZO-1 function leading to increased endothelial permeability, inflammatory cell transmigration and initiation of atherosclerosis
- Discover the involvement of stress signaling JNK and p38 pathways in pathological suppression of thrombomodulin, a vascular protective molecule, downregulated in many thrombotic and vascular diseases
- Link uremic toxins (in particular, PAA) in patients with chronic liver disease to increased ROS production and stimulation of TNF-a in endothelial cells leading to atherosclerosis and vascular calcification
- Demonstrate that in diabetes, advanced glycation end products lead to ROS generation in endothelia via sustained NF-kB activation, contributing to progression of atherosclerosis
- Discover that CD40 ligand promotes monocyte adhesion to endothelial cells via PKCa, NF-kB and VCAM-1 signaling cascade, explaining the role of CD40L in atherogenesis
- Show that monocytes activated by endothelial cells, produce CD80 signaling that leads to allogenic immune response, indicating the need for specific therapy to prevent monocyte activation during allograft transplantation
- Identify tetraspanin CD82 as the recognition sensor responsible for rejection of xenotransplants
- Develop 3d endothelialized engineered tissues, as well as new technology based on novel material surfaces and drugs (such as paclitaxel, sirolimus, vitamin C, C6-ceramide and 17β-estradiol) to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants
Additionally, HAOEC (along with human subclavian artery (HScAEC), carotid artery (HCtAEC), coronary artery (HCAEC) and brachiocephalic artery (HBcAEC), all provided by Cell Applications, Inc.) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response. The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors. To eliminate the donor-to-donor variability, the scientists took advantage of the great variety of primary cells offered by Cell Applications, including the option of ordering a panel of endothelial cells obtained from different vascular beds of the same donor!
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
Normal healthy human aorta
No bacteria, yeast, fungi, mycoplasma
Factor VIII-related Ag, DiI-Ac-LDL uptake
Attach, spread, proliferate in Growth Med
500,000 HAOEC (2nd passage) frozen in Basal Medium w/10% FBS, 10% DMSO
Cryovial frozen HAOEC (304-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K)
Shipped in Tsfr Med, 3rd psg (flasks or plates)
At least 16
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved HAOEC Total Kit: 5x10^5 Cells (Adult), Media & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 304K-05a||Price: $855.22|
|Cryopreserved HAOEC Total Kit, Pre-Screened: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: S304K-05a||Price: $961.20|
|Cryopreserved HAOEC, adult: Frozen HAOEC (5x10^5)||Size: 1 Cryovial||CAT.#: 304-05a||Price: $698.49|
|Cryopreserved HAOEC, adult, Pre-Screened: Frozen HAOEC (5x10^5)||Size: 1 Cryovial||CAT.#: S304-05a||Price: $807.19|
|Cryopreserved HAOEC-AS Total Kit: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 304ASK-05a||Price: $924.72|
|Cryopreserved HAOEC-AS, adult: Frozen HAOEC-AS from donor with Asthma (5x10^5)||Size: 1 Cryovial||CAT.#: 304AS-05a||Price: $769.78|
|Cryopreserved HAOEC-T2D Total Kit: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 304T2DK-05a||Price: $924.47|
|Cryopreserved HAOEC-T2D, adult: Frozen HAOEC from donor with Type 2 Diabetes, Adult (5x10^5)||Size: 1 Cryovial||CAT.#: 304T2D-05a||Price: $769.52|
|Proliferating HAOEC, adult: Actively growing, dividing cells in medium||Size: T-25 Flask||CAT.#: 305-25a||Price: $698.49|
|Proliferating HAOEC, adult: Actively growing, dividing cells in medium||Size: T-75 Flask||CAT.#: 305-75a||Price: $902.97|
|Proliferating HAOEC, adult: Actively growing, dividing cells in medium||Size: 6 Well||CAT.#: 305-6Wa||Price: $902.97|
|Proliferating HAOEC, adult: Actively growing, dividing cells in medium||Size: 96 Well||CAT.#: 305-96Wa||Price: $1,032.12|
|Proliferating HAOEC, adult, Pre-Screened: Actively growing, dividing cells in medium||Size: T-25 Flask||CAT.#: S305-25a||Price: $807.19|
|Proliferating HAOEC, adult, Pre-Screened: Actively growing, dividing cells in medium||Size: T-75 Flask||CAT.#: S305-75a||Price: $1,011.68|
|Proliferating HAOEC, adult, Pre-Screened: Actively growing, dividing cells in medium||Size: 6 Well||CAT.#: S305-6Wa||Price: $1,011.68|
|Proliferating HAOEC, adult, Pre-Screened: Actively growing, dividing cells in medium||Size: 96 Well||CAT.#: S305-96Wa||Price: $1,140.83|
|Human Endothelial Cell Basal Medium: Basal medium without growth supplement and phenol red||Size: 500 ml||CAT.#: 210PR-500||Price: $75.34|
|Human Endothelial Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: 210-500||Price: $69.96|
|Human Endothelial Cell Defined Medium: Defined medium without serum||Size: 500 ml||CAT.#: 113-500||Price: $113.01|
|Human Endothelial Cell Defined Medium: Defined medium without serum and phenol red||Size: 500 ml||CAT.#: 113PR-500||Price: $118.39|
|Human Endothelial Cell Growth Medium: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: 500 ml||CAT.#: 211PR-500||Price: $113.01|
|Human Endothelial Cell Growth Medium: Growth medium without FBS. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: 500 ml||CAT.#: 211F-500||Price: $113.01|
|Human Endothelial Cell Growth Medium: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: 500 ml||CAT.#: 211A-500||Price: $113.01|
|Human Endothelial Cell Growth Medium: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: 500 ml||CAT.#: 211H-500||Price: $113.01|
|Human Endothelial Cell Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: 500 ml||CAT.#: 211-500||Price: $105.47|
|Human Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: Yields 500 ml||CAT.#: 211K-500||Price: $123.77|
|Human Endothelial Cell Growth Medium v2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500.||Size: 500 ml||CAT.#: 213-500||Price: $123.00|
|Human Endothelial Cell Growth Supplement: Growth supplement without FBS||Size: 5 ml||CAT.#: 211F-GS||Price: $69.96|
|Human Endothelial Cell Growth Supplement: Growth supplement without antibiotics||Size: 15 ml||CAT.#: 211A-GS||Price: $67.80|
|Human Endothelial Cell Growth Supplement: Growth supplement without hydrocortisone||Size: 15 ml||CAT.#: 211H-GS||Price: $67.80|
|Human Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.||Size: 15 ml||CAT.#: 211-GS||Price: $57.04|
|Human Endothelial Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment||Size: 250 ml||CAT.#: 209-250||Price: $69.96|
|Human Endothelial Cell Starvation Medium: Starvation medium without phenol red||Size: 250 ml||CAT.#: 209PR-250||Price: $78.57|
Extended Family Products
|Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody||Size: 100 ul||CAT.#: CG1238||Price: $275.00|
|Anti-VEGF: Polyclonal Vascular Endothelial Growth Factor Antibody||Size: 100 ul||CAT.#: CA1080||Price: $302.38|
|Anti-VEGF-C: Polyclonal Vascular Endothelial Growth Factor-C Antibody||Size: 100 ul||CAT.#: CB3778||Price: $302.38|
|Anti-VEGFR1: Polyclonal VEGF Receptor 1 Antibody||Size: 100 ul||CAT.#: CB3839||Price: $333.13|
|Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $53.81|
|Cyto-X Colorimetric Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $138.84|
|Cyto-X Colorimetric Cell Counting Reagent: 100 tests||Size: Sample||CAT.#: 028-S||Price: $35.88|
|Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF101K||Price: $430.50|
|Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF101KS||Price: $53.76|
|E-Selectin ELISA Kit, Human: Human E-Selectin ELISA Kit||Size: 96 Wells||CAT.#: CL0501||Price: $586.56|
|HAOEC RNA: Total RNA prepared from Human Aortic Endothelial Cells, adult||Size: 10 ug||CAT.#: 304-R10a||Price: $349.78|
|HAOEC RNA: Total RNA prepared from Human Aortic Endothelial Cells, adult||Size: 25 ug||CAT.#: 304-R25a||Price: $699.56|
|Human Heart RNA: Total RNA prepared from human heart tissue||Size: 50 ug||CAT.#: 1H30-50||Price: $138.57|
|Human Heart RNA: Total RNA prepared from human heart tissue||Size: 250 ug||CAT.#: 1H30-250||Price: $521.98|
|ICAM-1 ELISA Kit, Human: Human Intercellular Adhesion Molecule-1 ELISA Kit||Size: 96 Wells||CAT.#: CL0370||Price: $484.31|
|IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10||Size: 25 ug||CAT.#: RP1127-25||Price: $193.73|
|IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10||Size: 100 ug||CAT.#: RP1127-100||Price: $484.31|
|IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10||Size: 1000 ug||CAT.#: RP1127-1000||Price: $3,174.94|
|P-Selectin ELISA Kit, Human: Human P-Selectin ELISA Kit||Size: 96 wells||CAT.#: CL0505||Price: $516.60|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $54.89|
|VEGF-121: Human Vascular Endothelial Growth Factor-121||Size: 10 ug||CAT.#: RP1116-10||Price: $193.73|
|VEGF-121: Human Vascular Endothelial Growth Factor-121||Size: 100 ug||CAT.#: RP1116-100||Price: $484.31|
|VEGF-121: Human Vascular Endothelial Growth Factor-121||Size: 1000 ug||CAT.#: RP1116-1000||Price: $4,089.75|
|VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free||Size: 10 ug||CAT.#: RP1116AF-10||Price: $213.10|
|VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free||Size: 100 ug||CAT.#: RP1116AF-100||Price: $532.74|
|VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free||Size: 1000 ug||CAT.#: RP1116AF-1000||Price: $4,498.73|
|VEGF-C ELISA Kit, Human: Human Vascular Endothelial Growth Factor C ELISA Kit||Size: 96 Wells||CAT.#: CL0588||Price: $581.18|
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Hung, M., Y. Kao, C. Mao, T. Chen and W. Chen. 2016. Aliskiren attenuates the effects of interleukin-6 on endothelial nitric oxide synthase and caveolin-1 in human aortic endothelial cells. Nitric Oxide, 61:45-54.
Lamichhane, S., J. Anderson, T. Remund, H. Sun, M. Larson, P. Kelly and G. Mani. 2016. Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene. J Biomed Mat Res, 104:2291-2304.
Wang, H., S. Chen and W. Lo. 2016. Identification of Cofilin-1 Induces G0/G1 Arrest and Autophagy in Angiotensin-(1-7)-treated Human Aortic Endothelial Cells from iTRAQ Quantitative Proteomics. Scientific Reports, 6, 35372.
Wang, Y. W. Nie, K. Yao, Z. Wang andn H. He. 2016. Interleukin 6 induces expression of NADPH oxidase 2 in human aortic endothelial cells via long noncoding RNA MALAT1. Die Pharmazie, 71:592-597.
Anderson, J., T. Remund, K. Pohlson, S. Lamichhane, C. Evans, R. Evans, M. Clark, K. Egland, P. Kelly and G. Mani. 2015. In vitro and in vivo evaluation of effect of excipients in local delivery of paclitaxel using microporous infusion balloon catheters. J Biomed Mater Res Part B, DOI: 10.1002/jbm.b.33564.
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Wang, H., S. Chen, and W. Lo. 2015. iTRAQ quantitative proteomics-based identification of cell adhesion as a dominant phenotypic modulation in thrombin-stimulated human aortic endothelial cells. Thrombosis Research, 135:944-950.
Chang, M., C. Tsao, W. Huang, P. Chen, and S. Hung. 2014. Conditioned medium derived from mesenchymal stem cells overexpressing HPV16 E6E7 dramatically improves ischemic limb. Journal of Molecular and Cellular Cardiology, 72:339–349.
Fu, X., X. Huang, P. Li, W. Chen, and M. Xia. 2014. 7-Ketocholesterol inhibits isocitrate dehydrogenase 2 expression and impairs endothelial function via microRNA-144. Free Radical Biology and Medicine, 71:1-15.
Gardner, A., D. Parker, P. Montgomery, D. Sosnowska, A. Casanegra, Z. Ungvari, A. Csiszar, and W. Sonntag. 2014. Gender and racial differences in endothelial oxidative stress and inflammation in patients with symptomatic peripheral artery disease. Journal of Vascular Surgery, 3 April.
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Liu, Y., D. Li, Y. Zhang, R. Sun and M. Xia. 2014. Anthocyanin increases adiponectin secretion and protects against diabetes-related endothelial dysfunction. Am J Physiol – Endocrinol & Metab, 306:E9.
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Morita, M., S. Yano, T. Yamaguchi, and T. Sugimoto. 2013. Advanced glycation end products-induced reactive oxygen species generation is partly through NF-kappa B activation in human aortic endothelial cells. Journal of Diabetes and its Complications. 27:11-15.
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Whitsett, J., A. Rangel Filho, S. Sethumadhavan, J. Celinska, M. Widlansky, and J. Vasquez-Vivar. 2013. Human endothelial dihydrofolate reductase low activity limits vascular tetrahydrobiopterin recycling. Free Radical Biology and Medicine. 63:143-150.
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Li, X., Y. Rong, M. Zhang, X.L. Wang, S.A. LeMaire, J.S. Coselli, Y. Zhang, and Y.H. Shen. 2009. Up-regulation of thioredoxin interacting protein (Txnip) by p38 MAPK and FOXO1 contributes to the impaired thioredoxin activity and increased ROS in glucose-treated endothelial cells. Biochemical and biophysical research communications. 381:660-665.
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Wang, H.-J., H. Huang, Y.-C. Chuang, and H.-C. Huang. 2009. Paclitaxel induces up-regulation of tissue factor in human aortic endothelial cells. International Immunopharmacology. 9:144-147.
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