MSDS Cryopreserved Cells
Instructions HAOEC Normal
Instructions HAOEC-T2D
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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Description
Human Aortic Endothelial Cells (HAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications to study diabetes-associated complications related to cardiovascular function, investigate mechanisms of immune response and graft rejection, study endothelial dysfunction caused by air pollution, oxidative stress and inflammation, and develop 3d endothelialized engineered tissues, as well as new technologies based on novel material surfaces and drugs in order to reduce risks associated with vascular implants.
Select HAOEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx). More information about pre-screened endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
HAOEC from Cell Applications, Inc. have been used to:
- Demonstrate that increased glucose flux leads to endothelial dysfunction in diabetes via activating Egr1-mediated proinflammatory and prothrombotic responses
- Study apoptosis, oxidative stress and inflammation associated with atherosclerosis and demonstrate the beneficial effects of anthocyanin on endothelial cells damaged by exposure to oxidized sterols
- Demonstrate that upregulation of thioredoxin via AMPK-FOXO3 pathway protects endothelial cells from oxidative stress and may prevent cardiovascular diseases in patients with metabolic syndrome and diabetes and further elucidate the involvement of AMPK cascade in mediating beneficial cardiovascular effects of green tea
- Test anti-inflammatory and vasodilating properties of a synthetic rutaecarpine derivative
- Show that glycated albumin, associated with diabetic complications, decreases endothelial miR-146a expression which leads to increased IL-6 production, and that angiotensin protects endothelial cells by preventing miR-146a downregulation
- Demonstrate that air pollutants can directly affect ZO-1 function leading to increased endothelial permeability, inflammatory cell transmigration and initiation of atherosclerosis
- Discover the involvement of stress signaling JNK and p38 pathways in pathological suppression of thrombomodulin, a vascular protective molecule, downregulated in many thrombotic and vascular diseases
- Link uremic toxins (in particular, PAA) in patients with chronic liver disease to increased ROS production and stimulation of TNF-a in endothelial cells leading to atherosclerosis and vascular calcification
- Demonstrate that in diabetes, advanced glycation end products lead to ROS generation in endothelia via sustained NF-kB activation, contributing to progression of atherosclerosis
- Discover that CD40 ligand promotes monocyte adhesion to endothelial cells via PKCa, NF-kB and VCAM-1 signaling cascade, explaining the role of CD40L in atherogenesis
- Show that monocytes activated by endothelial cells, produce CD80 signaling that leads to allogenic immune response, indicating the need for specific therapy to prevent monocyte activation during allograft transplantation
- Identify tetraspanin CD82 as the recognition sensor responsible for rejection of xenotransplants
- Develop 3d endothelialized engineered tissues, as well as new technology based on novel material surfaces and drugs (such as paclitaxel, sirolimus, vitamin C, C6-ceramide and 17β-estradiol) to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants
Additionally, HAOEC (along with human subclavian artery (HScAEC), carotid artery (HCtAEC), coronary artery (HCAEC) and brachiocephalic artery (HBcAEC), all provided by Cell Applications, Inc.) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response. The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors. To eliminate the donor-to-donor variability, the scientists took advantage of the great variety of primary cells offered by Cell Applications, including the option of ordering a panel of endothelial cells obtained from different vascular beds of the same donor!
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
Human Aortic Endothelial Cells: HAOEC (A). HAOEC immunolabeled with Anti-CD31 antibodies (green); nuclei are counterstained with PI (red) (B). HAOEC (C) transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (D).
Details
Tissue | Normal healthy human aorta |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Factor VIII-related Ag, DiI-Ac-LDL uptake. S-HAOEC are select HAOEC lots that have been tested positive for VEGFR2 pathway activation following stimulation by VEGF. |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HAOEC (2nd passage) frozen in Basal Medium w/10% FBS, 10% DMSO |
Kit | Cryovial frozen HAOEC (304-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, 3rd psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Aortic Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Media & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 304K-05a | Price: $904.00 | |
| Cryopreserved Aortic Endothelial Cells (HAOEC), adult: Frozen HAOEC (5x10^5) | Size: 1 Cryovial | CAT.#: 304-05a | Price: $730.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: S305-25a | Price: $896.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: S305-75a | Price: $1,086.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: S305-24Wa | Price: $1,086.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: S305-96Wa | Price: $1,206.00 | |
| Cryopreserved Pre-Screened Human Aortic Endothelial Cells Total Kit: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S304K-05a | Price: $1,070.00 | |
| Cryopreserved Aortic Endothelial Cells (HAOEC), Pre-Screened, adult: Frozen HAOEC (5x10^5) | Size: 1 Cryovial | CAT.#: S304-05a | Price: $896.00 | |
| Cryopreserved Aortic Endothelial Cells, Asthma (HAOEC-AS), adult: Frozen HAOEC-AS from donor with Asthma (5x10^5) | Size: 1 Cryovial | CAT.#: 304AS-05a | Price: $925.00 | |
| Cryopreserved Human Aortic Endothelial Cells with Asthma Total Kit, adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 304ASK-05a | Price: $1,099.00 | |
| Cryopreserved Aortic Endothelial Cells, Type 2 Diabetes (HAOEC-T2D), adult: Frozen HAOEC from donor with Type 2 Diabetes, Adult (5x10^5) | Size: 1 Cryovial | CAT.#: 304T2D-05a | Price: $925.00 | |
| Cryopreserved Human Aortic Endothelial Cells with Type 2 Diabetes Total Kit, adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 304T2DK-05a | Price: $1,099.00 | |
| Proliferating Aortic Endothelial Cells (HAOEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 305-25a | Price: $730.00 | |
| Proliferating Aortic Endothelial Cells (HAOEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 305-75a | Price: $920.00 | |
| Proliferating Aortic Endothelial Cells (HAOEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 305-24Wa | Price: $920.00 | |
| Proliferating Aortic Endothelial Cells (HAOEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 305-96Wa | Price: $1,040.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 210-500 | Price: $91.00 | |
| Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in confluent cultures. Not for cell growth. | Size: 500 ml | CAT.#: 113-500 | Price: $130.00 | |
| Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. | Size: 500 ml | CAT.#: 113PR-500 | Price: $136.00 | |
| Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211-500 | Price: $121.00 | |
| Human EC Growth Medium wo Phenol Red: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211PR-500 | Price: $130.00 | |
| Human EC Growth Medium wo FBS: 211-500 growth medium, but without FBS. FBS must be added in order to support cell culture. | Size: 500 ml | CAT.#: 211F-500 | Price: $130.00 | |
| Human EC Growth Medium wo Antibiotics: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. Suitable for Cytofect Transfection Kit. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211A-500 | Price: $130.00 | |
| Human EC Growth Medium wo Hydrocortisone: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211H-500 | Price: $130.00 | |
| Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: Yields 500 ml | CAT.#: 211K-500 | Price: $143.00 | |
| Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 15 ml | CAT.#: 211-GS | Price: $74.00 | |
| Human EC Growth Supplement wo FBS: Growth supplement without FBS. FBS must be added in order to support cell culture. | Size: 5 ml | CAT.#: 211F-GS | Price: $91.00 | |
| Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics | Size: 15 ml | CAT.#: 211A-GS | Price: $88.00 | |
| Human EC Growth Supplement wo Hydrocortisone: Growth supplement without hydrocortisone | Size: 15 ml | CAT.#: 211H-GS | Price: $88.00 | |
| Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | Size: 250 ml | CAT.#: 209-250 | Price: $81.00 | |
| Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red | Size: 250 ml | CAT.#: 209PR-250 | Price: $91.00 | |
| Human EC Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. | Size: 500 ml | CAT.#: 213-500 | Price: $141.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody | Size: 100 ul | CAT.#: CA1080 | Price: $302.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Aortic Endothelial Cell RNA (HAOEC RNA), Adult: Total RNA prepared from Human Aortic Endothelial Cells, adult | Size: 10 ug | CAT.#: 304-R10a | Price: $398.00 | |
| Aortic Endothelial Cell RNA (HAOEC RNA), Adult: Total RNA prepared from Human Aortic Endothelial Cells, adult | Size: 25 ug | CAT.#: 304-R25a | Price: $796.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2016
Chiang, E. H. Ma, J. Wang, C. Liu, T. Chen andn S. Hung. 2016. Multi-lineage differentiation and angiogenesis potentials of pigmented villonodular synovitis derived mesenchymal stem cells - pathological implication. J Orth Res, 34:395-403.
Hung, M., Y. Kao, C. Mao, T. Chen and W. Chen. 2016. Aliskiren attenuates the effects of interleukin-6 on endothelial nitric oxide synthase and caveolin-1 in human aortic endothelial cells. Nitric Oxide, 61:45-54.
Lamichhane, S., J. Anderson, T. Remund, H. Sun, M. Larson, P. Kelly and G. Mani. 2016. Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene. J Biomed Mat Res, 104:2291-2304.
Wang, H., S. Chen and W. Lo. 2016. Identification of Cofilin-1 Induces G0/G1 Arrest and Autophagy in Angiotensin-(1-7)-treated Human Aortic Endothelial Cells from iTRAQ Quantitative Proteomics. Scientific Reports, 6, 35372.
Wang, Y. W. Nie, K. Yao, Z. Wang andn H. He. 2016. Interleukin 6 induces expression of NADPH oxidase 2 in human aortic endothelial cells via long noncoding RNA MALAT1. Die Pharmazie, 71:592-597.
2015
Anderson, J., T. Remund, K. Pohlson, S. Lamichhane, C. Evans, R. Evans, M. Clark, K. Egland, P. Kelly and G. Mani. 2015. In vitro and in vivo evaluation of effect of excipients in local delivery of paclitaxel using microporous infusion balloon catheters. J Biomed Mater Res Part B, DOI: 10.1002/jbm.b.33564.
Chang, E., H. Ma, J. Wang, C. Liu, T. Chen, and S Hung. 2015. Multi-lineage differentiation and angiogenesis potentials of pigmented villonodular synovitis derived mesenchymal stem cells - pathological implication. J Orthopaedic Res, DOI: 10.1002/jor.23031.
Li, R., N. Jen, L. Wu, J. Lee, K. Fang, K. Quigley, K. Lee, S. Wang, B. Zhou, L. Vergnes, Y. Chen, Z. Li, K. Reue, D. Ann, and T. Hsiai. 2015. Disturbed Flow Induces Autophagy But Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis. Antioxidants & Redox Signaling, doi:10.1089/ars.2014.5896.
Nelson, J. 2015. Dynamic Blood Flow Modulates Endothelial Mitochondrial Redox States and Vascular Repair. PhD Dissertation, UCLA.
Wang, H., S. Chen, and W. Lo. 2015. iTRAQ quantitative proteomics-based identification of cell adhesion as a dominant phenotypic modulation in thrombin-stimulated human aortic endothelial cells. Thrombosis Research, 135:944-950.
2014
Chang, M., C. Tsao, W. Huang, P. Chen, and S. Hung. 2014. Conditioned medium derived from mesenchymal stem cells overexpressing HPV16 E6E7 dramatically improves ischemic limb. Journal of Molecular and Cellular Cardiology, 72:339–349.
Fu, X., X. Huang, P. Li, W. Chen, and M. Xia. 2014. 7-Ketocholesterol inhibits isocitrate dehydrogenase 2 expression and impairs endothelial function via microRNA-144. Free Radical Biology and Medicine, 71:1-15.
Gardner, A., D. Parker, P. Montgomery, D. Sosnowska, A. Casanegra, Z. Ungvari, A. Csiszar, and W. Sonntag. 2014. Gender and racial differences in endothelial oxidative stress and inflammation in patients with symptomatic peripheral artery disease. Journal of Vascular Surgery, 3 April.
Habib, A., V. Karmali, M. John, R. Polavarapu, G. Nakazawa, K. Pachura, T. Davis, F. Kolodgie, R. Virmani and A. Finn. 2014. Everolimus-Eluting Stents Improve Vascular Response in a Diabetic Animal Model. Circulation: Cardiovascular Interventions, 7:526-532.
Liu, Y., D. Li, Y. Zhang, R. Sun and M. Xia. 2014. Anthocyanin increases adiponectin secretion and protects against diabetes-related endothelial dysfunction. Am J Physiol – Endocrinol & Metab, 306:E9.
Tian, S., X. Ge, K. Wu, H. Yang and Y. Liu. 2014. Ramipril Protects the Endothelium from High Glucose–Induced Dysfunction through CaMKKβ/AMPK and Heme Oxygenase-1 Activation. J Pharmacol & Exp Ther, 350:5-13.
Wang, H., Y. Huang, Y. Shih, H. W, C. Peng, and W. Lo. 2014. MicroRNA-146a decreases high glucose/thrombin-induced endothelial inflammation by inhibiting NAPDH oxidase 4 expression. Mediators of Inflammation, Volume 2014, Article ID 379537.
2013
Deshpande, D., D.R. Janero, and M. Amiji. 2013. Engineering of an ω-3 polyunsaturated fatty acid-containing nanoemulsion system for combination C6-ceramide and 17β-estradiol delivery and bioactivity in human vascular endothelial and smooth muscle cells. Nanomedicine: Nanotechnology, Biology and Medicine. 9:885-894.
Kakade, S., and G. Mani. 2013. A comparative study of the effects of vitamin C, sirolimus, and paclitaxel on the growth of endothelial and smooth muscle cells for cardiovascular medical device applications. Drug design, development and therapy. 7:529.
Lamichhane, S., S. Lancaster, E. Thiruppathi, and G. Mani. 2013. Interaction of Endothelial and Smooth Muscle Cells with Cobalt–Chromium Alloy Surfaces Coated with Paclitaxel Deposited Self-Assembled Monolayers. Langmuir. 29:14254-14264.
Lee, C.-M., J.-A. Gu, T.-G. Rau, C.-H. Yang, W.-C. Yang, S.-H. Huang, F.-Y. Lin, C.-M. Lin, and S.-T. Huang. 2013. Low-Cytotoxic Synthetic Bromorutaecarpine Exhibits Anti-Inflammation and Activation of Transient Receptor Potential Vanilloid Type 1 Activities. BioMed Research International. 2013:Article ID 795095.
Morita, M., S. Yano, T. Yamaguchi, and T. Sugimoto. 2013. Advanced glycation end products-induced reactive oxygen species generation is partly through NF-kappa B activation in human aortic endothelial cells. Journal of Diabetes and its Complications. 27:11-15.
Saleh, S.M., R.S. Parhar, R.S. Al-Hejailan, R.H. Bakheet, H.S. Khaleel, H.G. Khalak, A.S. Halees, M.Z. Zaidi, B.F. Meyer, and G.P. Yung. 2013. Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation. The Journal of Immunology. 191:2796-2805.
Scott, D.W., M.O. Vallejo, and R.P. Patel. 2013. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. Journal of the American Heart Association. 2:e000263-e000263.
Vedantham, S., D. Thiagarajan, R. Ananthakrishnan, L. Wang, R. Rosario, Y.S. Zou, I. Goldberg, S.F. Yan, A.M. Schmidt, and R. Ramasamy. 2013. Aldose Reductase drives hyperacetylation of Egr-1 in hyperglycemia and consequent upregulation of proinflammatory and prothrombotic signals. Diabetes:db13-0032.
Wang, H.-J., W.-Y. Lo, and L.-J. Lin. 2013. Angiotensin-(1–7) decreases glycated albumin-induced endothelial interleukin-6 expression via modulation of miR-146a. Biochemical and biophysical research communications. 430:1157-1163.
Whitsett, J., A. Rangel Filho, S. Sethumadhavan, J. Celinska, M. Widlansky, and J. Vasquez-Vivar. 2013. Human endothelial dihydrofolate reductase low activity limits vascular tetrahydrobiopterin recycling. Free Radical Biology and Medicine. 63:143-150.
Wu, Z., G. Zhao, L. Peng, J. Du, S. Wang, Y. Huang, J. Ou, and Z. Jian. 2013. Protein Kinase C beta Mediates CD40 Ligand-Induced Adhesion of Monocytes to Endothelial Cells. PloS one. 8:e72593.
2012
Eid, N. 2012. FSTL-1 SECRETED BY MESENCHYMAL STEM CELLS INCREASES CELL VIABILITY OF HUMAN AORTIC ENDOTHELIAL CELLS UNDER HYPOXIC STRESS. BA Thesis, Wilkes Honors College of Florida Atlantic University. EC Gr Med, EC Basal Med
Kanie, K., Y. Narita, F. Kuwabara, M. Satake, S. Honda, H. Kaneko, H. Honda, and R. Kato. 2012. Cell-Selective Peptide Distribution in Human Collagen Proteins. Kobunshi Ronbunshu, 69:129-134.
Kanie, K., Y. Narita, Y. Zhao, F. Kuwabara, M. Satake, S. Honda, H. Kaneko, T. Yoshioka, M. Okochi, H. Honda, and R. Kato. 2012. Collagen type IV-specific tripeptides for selective adhesion of endothelial and smooth muscle cells. Biotechnology and Bioengineering. 109:1808-1816.
Sakai, S., H. Inagaki, Y. Liu, T. Matsuyama, T. Kihara, J. Miyake, K. Kawakami, and M. Taya. 2012. Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures. Biotechnology and Bioengineering. 109:2911-2919.
Wang, H.-J., H.-C. Huang, Y.-C. Chuang, P.-J. Liao, D.-M. Yang, W. Yang, and H. Huang. 2012. Modulation of tissue factor and thrombomodulin expression in human aortic endothelial cells incubated with high glucose. Acta Diabetol. 49:125-130.
Wang, Y., Y. Zhang, X. Wang, Y. Liu, and M. Xia. 2012. Cyanidin-3-O-β-glucoside induces oxysterol efflux from endothelial cells: Role of liver X receptor alpha. Atherosclerosis. 223:299-305.
2011
Wang, H.-J., T.-L. Lu, H. Huang, and H.-C. Huang. 2011. Paclitaxel induces thrombomodulin downregulation in human aortic endothelial cells. Texas Heart Institute Journal. 38:20.
Yew, T.-L., Y.-T. Hung, H.-Y. Li, H.-W. Chen, L.-L. Chen, K.-S. Tsai, S.-H. Chiou, K.-C. Chao, T.-F. Huang, H.-L. Chen, and S.-C. Hung. 2011. Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation. Cell Transplantation. 20:693-706.
2010
Gelissen, I.C., S. Cartland, A.J. Brown, C. Sandoval, M. Kim, D.L. Dinnes, Y. Lee, V. Hsieh, K. Gaus, L. Kritharides, and W. Jessup. 2010. Expression and stability of two isoforms of ABCG1 in human vascular cells. Atherosclerosis. 208:75-82.
Hou, X., J. Song, X.-N. Li, L. Zhang, X. Wang, L. Chen, and Y.H. Shen. 2010. Metformin reduces intracellular reactive oxygen species levels by upregulating expression of the antioxidant thioredoxin via the AMPK-FOXO3 pathway. Biochemical and biophysical research communications. 396:199-205.
Li, R., Z. Ning, J. Cui, F. Yu, C. Sioutas, and T. Hsiai. 2010. Diesel exhaust particles modulate vascular endothelial cell permeability: Implication of ZO-1 expression. Toxicology letters. 197:163-168.
Reiter, C.E.N., J.-a. Kim, and M.J. Quon. 2010. Green Tea Polyphenol Epigallocatechin Gallate Reduces Endothelin-1 Expression and Secretion in Vascular Endothelial Cells: Roles for AMP-Activated Protein Kinase, Akt, and FOXO1. Endocrinology. 151:103-114.
Rong, Y., M. Zhang, L. Zhang, X.L. Wang, and Y.H. Shen. 2010. JNK-ATF-2 inhibits thrombomodulin (TM) expression by recruiting histone deacetylase4 (HDAC4) and forming a transcriptional repression complex in the TM promoter. FEBS letters. 584:852-858.
Wang, H.-J., W.-Y. Lo, T.-L. Lu, and H. Huang. 2010. (−)-Epigallocatechin-3-gallate decreases thrombin/paclitaxel-induced endothelial tissue factor expression via the inhibition of c-Jun terminal NH2 kinase phosphorylation. Biochemical and biophysical research communications. 391:716-721.
Wang, W. 2010. A Novel Role of HDAC5 in Flow-Induced Gene Expression. PhD Dissertation, U Rochester.
2009
Brito, L. 2009. Targeted endothelial nitric oxide synthase gene delivery with lipopolyplexes for the treatment of coronary restenosis. ProQuest.
Hampson, A. 2009. Transcriptional Regulation of Soluble Guanylyl Cyclase. B.Sc. honors thesis, Dublin Institute of Technology.
Husband, A., M. James, and N. Kumar. 2009. 6-substituted isoflavonoid compounds and uses thereof. Patent Application US 20120003270 A1.
Li, R., T. Beebe, J. Cui, M. Rouhanizadeh, L. Ai, P. Wang, M. Gundersen, W. Takabe, and T.K. Hsiai. 2009. Pulsatile shear stress increased mitochondrial membrane potential: Implication of Mn-SOD. Biochemical and biophysical research communications. 388:406-412.
Li, X., Y. Rong, M. Zhang, X.L. Wang, S.A. LeMaire, J.S. Coselli, Y. Zhang, and Y.H. Shen. 2009. Up-regulation of thioredoxin interacting protein (Txnip) by p38 MAPK and FOXO1 contributes to the impaired thioredoxin activity and increased ROS in glucose-treated endothelial cells. Biochemical and biophysical research communications. 381:660-665.
Li, X.-N., J. Song, L. Zhang, S.A. LeMaire, X. Hou, C. Zhang, J.S. Coselli, L. Chen, X.L. Wang, Y. Zhang, and Y.H. Shen. 2009. Activation of the AMPK-FOXO3 Pathway Reduces Fatty Acid–Induced Increase in Intracellular Reactive Oxygen Species by Upregulating Thioredoxin. Diabetes. 58:2246-2257.
Wang, H.-J., H. Huang, Y.-C. Chuang, and H.-C. Huang. 2009. Paclitaxel induces up-regulation of tissue factor in human aortic endothelial cells. International Immunopharmacology. 9:144-147.
2008
Brito, L., S. Little, R. Langer, and M. Amiji. 2008. Poly(β-amino ester) and Cationic Phospholipid-Based Lipopolyplexes for Gene Delivery and Transfection in Human Aortic Endothelial and Smooth Muscle Cells. Biomacromolecules. 9:1179-1187.
Heaton, A., N. Kumar, G. Kelly, and A. Husband. 2008. Compositions and therapeutic methods involving isoflavones and analogues thereof. Patent US 20090233999 A1.
Kim, C.-S., S.-B. Jung, A. Naqvi, T.A. Hoffman, J. DeRicco, T. Yamamori, M.P. Cole, B.-H. Jeon, and K. Irani. 2008. P53 Impairs Endothelium-Dependent Vasomotor Function Through Transcriptional Upregulation of P66shc. Circulation research. 103:1441-1450.
Maghzal, G.J., S.R. Thomas, N.H. Hunt, and R. Stocker. 2008. Cytochrome b5, Not Superoxide Anion Radical, Is a Major Reductant of Indoleamine 2,3-Dioxygenase in Human Cells. Journal of Biological Chemistry. 283:12014-12025.
Wang, H.-J., H.-C. Huang, Y.-C. Chuang, and H. Huang. 2008. Thrombin induces the expression of tissue factor in human aortic endothelial cells. Acta Cardiologica Sinica. 24:151 - 156.
Wang, X., Z. Liu, B. Zhu, P. Wang, C. Wu, and H. Xu. 2008. Molecular Characterization of Hypoxia-Hypothermia–Conditioned Human Endothelial Cells and Their Interaction With Human Monocytes. Transplantation Proceedings, 40:2127-2135.
Yamanaka, M., Y. Anada, Y. Igarashi, and A. Kihara. 2008. A splicing isoform of LPP1, LPP1a, exhibits high phosphatase activity toward FTY720 phosphate. Biochemical and biophysical research communications. 375:675-679.
Zhang, L., Y. Wu, Z. Jia, Y. Zhang, H.Y. Shen, and X. Li Wang. 2008. Protective effects of a compound herbal extract (Tong Xin Luo) on free fatty acid induced endothelial injury: Implications of antioxidant system. BMC complementary and alternative medicine. 8:39.
Zhu, B., Z. Liu, P. Wang, C. Wu, and H. Xu. 2008. A Nuclear Factor-κB Inhibitor BAY11-7082 Inhibits Interactions Between Human Endothelial Cells, T Cells, and Monocytes. Transplantation proceedings. 40:2724-2728.
2007
Hung, S.-C., R.R. Pochampally, S.-C. Chen, S.-C. Hsu, and D.J. Prockop. 2007. Angiogenic Effects of Human Multipotent Stromal Cell Conditioned Medium Activate the PI3K-Akt Pathway in Hypoxic Endothelial Cells to Inhibit Apoptosis, Increase Survival, and Stimulate Angiogenesis. Stem cells. 25:2363-2370.
Oitate, M., T. Hirota, T. Murai, S.-i. Miura, and T. Ikeda. 2007. Covalent Binding of Rofecoxib, but Not Other Cyclooxygenase-2 Inhibitors, to Allysine Aldehyde in Elastin of Human Aorta. Drug Metabolism and Disposition. 35:1846-1852.
Uchiyama, T., H. Atsuta, T. Utsugi, M. Oguri, A. Hasegawa, T. Nakamura, A. Nakai, M. Nakata, I. Maruyama, H. Tomura, F. Okajima, S. Tomono, S. Kawazu, R. Nagai, and M. Kurabayashi. 2007. HSF1 and constitutively active HSF1 improve vascular endothelial function (heat shock proteins improve vascular endothelial function). Atherosclerosis. 190:321-329.
Yamatake, K., M. Maeda, T. Kadowaki, R. Takii, T. Tsukuba, T. Ueno, E. Kominami, S. Yokota, and K. Yamamoto. 2007. Role for Gingipains in Porphyromonas gingivalis Traffic to Phagolysosomes and Survival in Human Aortic Endothelial Cells. Infection and Immunity. 75:2090-2100.
2006
Shen, Y.H., L. Zhang, B. Utama, J. Wang, Y. Gan, X. Wang, J. Wang, L. Chen, G.M. Vercellotti, J.S. Coselli, J.L. Mehta, and X.L. Wang. 2006. Human cytomegalovirus inhibits Akt-mediated eNOS activation through upregulating PTEN (phosphatase and tensin homolog deleted on chromosome 10). Cardiovascular Research. 69:502-511.
Shen, Y.H., L. Zhang, Y. Gan, X. Wang, J. Wang, S.A. LeMaire, J.S. Coselli, and X.L. Wang. 2006. Up-regulation of PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome Ten) Mediates p38 MAPK Stress Signal-induced Inhibition of Insulin Signaling: A CROSS-TALK BETWEEN STRESS SIGNALING AND INSULIN SIGNALING IN RESISTIN-TREATED HUMAN ENDOTHELIAL CELLS. Journal of Biological Chemistry. 281:7727-7736.
Uchiyama, T., H. Atsuta, T. Utsugi, Y. Ohyama, T. Nakamura, A. Nakai, M. Nakata, I. Maruyama, H. Tomura, F. Okajima, S. Tomono, S. Kawazu, R. Nagai, and M. Kurarbayashi. 2006. Simvastatin induces heat shock factor 1 in vascular endothelial cells. Atherosclerosis. 188:265-273.
Wang, J., Y.H. Shen, B. Utama, J. Wang, S.A. LeMaire, J.S. Coselli, G.M. Vercellotti, and X.L. Wang. 2006. HCMV infection attenuates hydrogen peroxide induced endothelial apoptosis – involvement of ERK pathway. FEBS Letters, 580:2779-2787.
Wang, X.L., L. Zhang, K. Youker, M.-X. Zhang, J. Wang, S.A. LeMaire, J.S. Coselli, and Y.H. Shen. 2006. Free Fatty Acids Inhibit Insulin Signaling–Stimulated Endothelial Nitric Oxide Synthase Activation Through Upregulating PTEN or Inhibiting Akt Kinase. Diabetes. 55:2301-2310.
Zhang, W.-Y., E. Schwartz, Y. Wang, J. Attrep, Z. Li, and P. Reaven. 2006. Elevated Concentrations of Nonesterified Fatty Acids Increase Monocyte Expression of CD11b and Adhesion to Endothelial Cells. Arteriosclerosis, Thrombosis, and Vascular Biology. 26:514-519.
2005
Nordskog, B.K., W.R. Fields, and G.M. Hellmann. 2005. Kinetic analysis of cytokine response to cigarette smoke condensate by human endothelial and monocytic cells. Toxicology. 212:87-97.
Owing, J. 2005. Smoking and health: new research. Nova Publishers.
Raveendran, M., J. Wang, D. Senthil, J. Wang, B. Utama, Y. Shen, D. Dudley, Y. Zhang, and X.L. Wang. 2005. Endogenous nitric oxide activation protects against cigarette smoking induced apoptosis in endothelial cells. FEBS letters. 579:733-740.
Xu, J., J. Zhou, N. Wang, and H. Xu. 2005. Effects of 4-hydroxy-2-nonenal on cultured human aortic endothelial cells and myocardial cell. Engineering in Medicine and Biology Society, IEEE-EMBS, 27th Intl Conf. 5598-5602, DOI: 10.1109/IEMBS.2005.1615755.
2004
Kenny, T.P., C.L. Keen, P. Jones, H.-J. Kung, H.H. Schmitz, and M.E. Gershwin. 2004. Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H2O2. Experimental biology and medicine. 229:765-771.
Nordskog, B., A. Blixt, A. Zieske, and G. Hellmann. 2004. MMP-1 polymorphic expression in aortic endothelial cells. Cardiovasc Toxicol. 4:75-83.
Raveendran, M., D. Senthil, B. Utama, Y. Shen, D. Dudley, J. Wang, Y. Zhang, and X.L. Wang. 2004. Cigarette suppresses the expression of P4Hα and vascular collagen production. Biochemical and biophysical research communications. 323:592-598.
Ryu, J.-W., K.H. Hong, J.H. Maeng, J.-B. Kim, J. Ko, J.Y. Park, K.-U. Lee, M.K. Hong, S.W. Park, Y.H. Kim, and K.H. Han. 2004. Overexpression of Uncoupling Protein 2 in THP1 Monocytes Inhibits β2 Integrin-Mediated Firm Adhesion and Transendothelial Migration. Arteriosclerosis, Thrombosis, and Vascular Biology. 24:864-870.
Shen, Y.H., B. Utama, J. Wang, M. Raveendran, D. Senthil, W.J. Waldman, J.D. Belcher, G. Vercellotti, D. Martin, B.M. Mitchelle, and X.L. Wang. 2004. Human Cytomegalovirus Causes Endothelial Injury Through the Ataxia Telangiectasia Mutant and p53 DNA Damage Signaling Pathways. Circulation research. 94:1310-1317.
2001
Barbosa, M., H. Brady, K. Chan, and J. Pardinas. 2001. Monitor therapeutic activity of estrogen modulator; obtain vascular endothelial cell, incubate with modulator, monitor expression of estrogen marker, presence of estrogen marker indicates modulator of cardiovascular activity. Patent Application US 20030054332 A1.