Instructions HAOSMC Normal
Instructions HAOSMC-T2D
MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
 
HAOSMC from Cell Applications, Inc. have been utilized in dozens of research studies, for example, to understand:
 
  • Cytokines and growth factor signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function
  • Hyperglycemia-related risk factors for atherosclerosis in diabetes patients, as well as effects of ethanol on vascular calcification
  • ECM deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering
  • Mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells
  • dvanced stent technology, including novel surface materials
  • Drug and gene delivery systems
  • Restenosis and other occlusive vasculopathies

Characterization: Positive for smooth muscle cell specific alpha-actin expression.

(Click to Enlarge) Human Aortic Smooth Muscle Cells, HAOSMC (A), immunolabeled for smooth muscle-specific alpha-actin by ABC method (red) (B) and by immunofluorescence (green) (C).  Nuclei are visualized with DAPI (blue) (C).

Details

Tissue
Normal healthy human aorta
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HAOSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Croyvial frozen HAOSMC (354-05a), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HAOSMC Normal

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Instructions HAOSMC-T2D

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HAOSMC Total Kit: 5x10^5 Cells (Pooled, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 354pK-05a Price: $802.75
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Cryopreserved HAOSMC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 354K-05a Price: $802.75
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Cryopreserved HAOSMC Total Kit: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 354K-05f Price: $855.22
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Cryopreserved HAOSMC, adult: Frozen HAOSMC (5x10^5) Size: 1 Cryovial CAT.#: 354-05a Price: $655.44
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Cryopreserved HAOSMC, adult, pooled: Frozen HAOSMC (5x10^5) Size: 1 Cryovial CAT.#: 354p-05a Price: $654.98
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Cryopreserved HAOSMC, fetal: Frozen HAOSMC (5x10^5) Size: 1 Cryovial CAT.#: 354-05f Price: $709.25
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Cryopreserved HAOSMC-T2D Total Kit: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 354T2DK-05a Price: $866.76
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Cryopreserved HAOSMC-T2D, adult: Frozen HAOSMC from donor with Type 2 Diabetes (5x10^5) Size: 1 Cryovial CAT.#: 354T2D-05a Price: $721.09
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Proliferating HAOSMC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 355-25a Price: $655.44
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Proliferating HAOSMC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 355-75a Price: $747.99
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Proliferating HAOSMC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 355-6Wa Price: $913.74
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Proliferating HAOSMC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 355-96Wa Price: $747.99
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Proliferating HAOSMC, fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 355-25f Price: $709.25
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Proliferating HAOSMC, fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 355-75f Price: $901.90
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Proliferating HAOSMC, fetal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 355-6Wf Price: $952.48
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Proliferating HAOSMC, fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 355-96Wf Price: $1,042.89
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Related Products

Product Size CAT.# Price Quantity
Human Smooth Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.04
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Human Smooth Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.19
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Human Smooth Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.47
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Human Smooth Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.01
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Human Smooth Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.04
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-IL-6: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.38
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.21
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $53.76
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HAOSMC RNA: Total RNA prepared from Human Aortic Smooth Muscle Cells, adult Size: 10 ug CAT.#: 354-R10a Price: $349.78
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HAOSMC RNA: Total RNA prepared from Human Aortic Smooth Muscle Cells, adult Size: 25 ug CAT.#: 354-R25a Price: $699.56
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $138.57
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $521.98
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IL-6: Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.31
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IL-6: Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,174.94
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IL-6: Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $193.73
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IL-6 ELISA Kit, Human: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $516.60
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.10
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $532.74
-+
IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.43
-+

Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2016
Becs, G., A. Zarjou, A. Agarwal, K. Éva Kovács, Á. Becs, M. Nyitrai, E. Balogh, E. Bányai, J. Eaton, P. Arosio, M. Poli, V. Jeney, J. Balla and G. Balla. 2016. Pharmacological induction of ferritin prevents osteoblastic transformation of smooth muscle cells. J Cellular & Molec Med, 20:217-230.
Franzoni, M., I. Cattaneo, L. Longaretti, M. Figliuzzi, B. Ene-Iordache and A. Remuzzi. 2016. Endothelial cell activation by hemodynamic shear stress derived from arteriovenous fistula for hemodialysis access. Am J Physiol Heart Circ Physiol 310:H49 –H59.
Lamichhane, S., J. Anderson, T. Remund, H. Sun, M. Larson, P. Kelly and G. Mani. 2016. Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene. J Biomed Mat Res, 104:2291-2304.
Maier, K., B. Ruhle, J. Stein, K. Gentile, F. Middleton and V. Gahtan. 2016. Thrombospondin-1 differentially regulates microRNAs in vascular smooth muscle cells. Molec & Cell Biochem, 412:111-117.
Mani, G., S. Lamichhane, J. Anderson, T. Remund and P. Kelly. 2016. Formulations for Tailored Drug Release. Patent Application US 20160121027 A1.
Oros, M. 2016. Ethanol accelerates vascular calcification in human aortic smooth muscle cells: Quantitative trait locus mapping reveals alcohol consumption genes. PhD Thesis, University of Debrecen, Doctoral School of Molecular and Immune Biology.
Wilhelmson, A., J. Fagman, I. Johansson, Z. Zou, A. Andersson, E. Svedlund, M. Johansson, P. Lindahl, P, Fogelstrand and Å. Tivesten. 2016. Increased Intimal Hyperplasia Following Vascular Injury in Male Androgen Receptor Deficient Mice. Endocrinology, doi: 10.1210/en.2016-1100.
2015
Anderson, J., T. Remund, K. Pohlson, S. Lamichhane, C. Evans, R. Evans, M. Clark, K. Egland, P. Kelly and G. Mani. 2015. In vitro and in vivo evaluation of effect of excipients in local delivery of paclitaxel using microporous infusion balloon catheters. J Biomed Mater Res Part B, DOI: 10.1002/jbm.b.33564.
Helkin, A., K. Maier, and V. Gahtan. 2015. Thrombospondin-1, -2 and -5 have differential effects on vascular smooth muscle cell physiology. BBRC, doi:10.1016/j.bbrc.2015.07.044.
Lamichhane, S., J. Anderson, T. Remund, P. Kelly and G. Mani. 2015. Dextran sulfate as a drug delivery platform for drug-coated balloons: Preparation, characterization, in vitro drug elution, and smooth muscle cell response. J Biomed Mater Res Part B, 104:1416-1430.
2014
Lavanya, V., C. Bashur and A. Ramamurthi. 2014. Impact of Cyclic Stretch on Induced Elastogenesis Within Collagenous Conduits. Tiss Eng Pt A, 20:1403-1415.
Seymour, K., J. Stein, X. Han, K. Maier, and V. Gahtan. 2014. Statins and Nitric Oxide Donors Affect Thrombospondin 1-Induced Chemotaxis.Vasc Endovascular Surg, 48:470-475.
Venkataraman, L, B. Sivaraman, P. Vaidya and A. Ramamurthi. 2014. Nanoparticulate delivery of agents for induced elastogenesis in three-dimensional collagenous matrices. J Tiss Eng & Regen Med, DOI: 10.1002/term.1889.
2013
Almontashiri, N.A.M., M. Fan, B.L.M. Cheng, H.-H. Chen, R. Roberts, and A.F.R. Stewart. 2013. Interferon-γ Activates Expression of p15 and p16 Regardless of 9p21.3 Coronary Artery Disease Risk Genotype. Journal of the American College of Cardiology. 61:143-147.
Deshpande, D., D.R. Janero, and M. Amiji. 2013. Engineering of an ω-3 polyunsaturated fatty acid-containing nanoemulsion system for combination C6-ceramide and 17β-estradiol delivery and bioactivity in human vascular endothelial and smooth muscle cells. Nanomedicine: Nanotechnology, Biology and Medicine. 9:885-894.
Hirase, T., H. Hara, Y. Miyazaki, N. Ide, A. Nishimoto-Hazuku, H. Fujimoto, C.J. Saris, H. Yoshida, and K. Node. 2013. Interleukin 27 inhibits atherosclerosis via immunoregulation of macrophages in mice. American Journal of Physiology-Heart and Circulatory Physiology. 305:H420-429.
Kakade, S., and G. Mani. 2013. A comparative study of the effects of vitamin C, sirolimus, and paclitaxel on the growth of endothelial and smooth muscle cells for cardiovascular medical device applications. Drug design, development and therapy. 7:529.
Lamichhane, S., S. Lancaster, E. Thiruppathi, and G. Mani. 2013. Interaction of Endothelial and Smooth Muscle Cells with Cobalt–Chromium Alloy Surfaces Coated with Paclitaxel Deposited Self-Assembled Monolayers. Langmuir. 29:14254-14264.
Gleason. 2013. Mechanism of aortic medial matrix remodeling is distinct in patients with bicuspid aortic valve. The Journal of thoracic and cardiovascular surgery: doi: 10.1016/j.jtcvs.2013.1004.1028.
Stein, J., C. Iwuchukwu, K. Maier, and V. Gahtan. 2013. Thrombospondin-1-induced smooth muscle cell chemotaxis and proliferation are dependent on transforming growth factor-β2 and hyaluronic acid synthase. Molecular and cellular biochemistry. 384:181-186.
Venkataraman, L., C.A. Bashur, and A. Ramamurthi. 2013. Impact of Cyclic Stretch on Induced Elastogenesis within Collagenous Conduits. Tissue Engineering Part A: doi:10.1089/ten.TEA.2013.0294.
2012
Acilan, C., M. Serhatli, O. Kacar, Z. Adiguzel, A. Tuncer, M. Hayran, and K. Baysal. 2012. Smooth Muscle Cells Isolated from Thoracic Aortic Aneurysms Exhibit Increased Genomic Damage, but Similar Tendency for Apoptosis. DNA Cell Biol, 31:1523-1534.
Almontashiri, N. 2012. A gain of function variant of the mitochondrial matrix protease SPG7 is associated with increased risk of coronary artery disease. Vol. MSc dissertation. University of Ottawa.
LaBerge, M. 2012. Induced Elastic Matrix Synthesis within 3-Dimensional Collagen Constructs. PhD Dissertation, Clemson U.
Oros, M., E. Zavaczki, C. Vadasz, V. Jeney, A. Tosaki, I. Lekli, G. Balla, L. Nagy, and J. Balla. 2012. Ethanol increases phosphate‐mediated mineralization and osteoblastic transformation of vascular smooth muscle cells. Journal of cellular and molecular medicine. 16:2219-2226.
Santiago, F.S. 2012. Regulatory Mechanisms in Vascular Injury and Repair. University of New South Wales, PhD dissertation.
Venkataraman, L. 2012. Induced elastic matrix synthesis within 3-dimensional collagen constructs. PhD dissertation, Clemson University.
2011
Chen, J., M.Y. Liu, C.R. Parish, B.H. Chong, and L. Khachigian. 2011. Nuclear import of early growth response-1 involves importin-7 and the novel nuclear localization signal serine-proline-serine. Intern. J. of biochemistry & cell biology. 43:905-912.
Gacchina, C., T. Brothers, and A. Ramamurthi. 2011. Evaluating smooth muscle cells from CaCl2-induced rat aortal expansions as a surrogate culture model for study of elastogenic induction of human aneurysmal cells. Tissue Engineering Part A. 17:1945-1958.
Liu, X., E.-Z. Jin, J.-X. Zhi, and X.-Q. Li. 2011. Identification of HZF1 as a novel target gene of the MEF2 transcription factor. Molecular medicine reports. 4:465-469.
2010
Brito, L.A., S. Chandrasekhar, S.R. Little, and M.M. Amiji. 2010. In vitro and In vivo studies of local arterial gene delivery and transfection using lipopolyplexes‐embedded stents. Journal of Biomedical Materials Research Part A. 93:325-336.
Gacchina, C.E. 2010. Cell-mediated elastic matrix regeneration toward regression of abdominal aortic aneurysms. PhD Dissertation, Clemson University.
Phillippi, J., M. Eskay, A. Kubala, B. Pitt and T. Gleason. 2010. Altered Oxidative Stress Responses and Increased Type I Collagen Expression in Bicuspid Aortic Valve Patients. Annals Thor Sug, 90:1893-1898.
Seymour, K., X. Han, B. Sadowitz, K.G. Maier, and V. Gahtan. 2010. Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells. The American J. Surgery. 200:615-619.
2009
Argraves, W., A. Tanaka, E. Smith, W. Twal, K. Argraves, D. Fan, and C. Haudenschild. 2009. Fibulin-1 and fibrinogen in human atherosclerotic lesions. Histochemistry and Cell Biology, 132:559-565.
Brito, L. 2009. Targeted endothelial nitric oxide synthase gene delivery with lipopolyplexes for the treatment of coronary restenosis. ProQuest.
Fischer, L.J., S. McIlhenny, T. Tulenko, N. Golesorkhi, P. Zhang, R. Larson, J. Lombardi, I. Shapiro, and P.J. DiMuzio. 2009. Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. Journal of Surgical Research. 152:157-166.
Hampson, A. 2009. Transcriptional Regulation of Soluble Guanylyl Cyclase. B.Sc. honors thesis, Dublin Institute of Technology.
Jiang, X., P.F. Austin, R.A. Niederhoff, S.R. Manson, J.J. Riehm, B.L. Cook, G. Pengue, K. Chitaley, K. Nakayama, and K.I. Nakayama. 2009. Mechanoregulation of proliferation. Molecular and cellular biology. 29:5104-5114.
Liu, M.Y., and L.M. Khachigian. 2009. HDAC-1 is Enriched at the PDGF-D Promoter in Response to IL-1beta and Forms a Cytokine-Inducible Gene Silencing Complex with NF-kappaB p65 and IRF-1. Journal of Biological Chemistry:10.1074/jbc.M1109.061903.
Morrow, D., J.P. Cullen, W. Liu, S. Guha, C. Sweeney, Y.A. Birney, N. Collins, D. Walls, E.M. Redmond, and P.A. Cahill. 2009. Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A. Arterioscler Thromb Vasc Biol. 29:1112-1118.
2008
Brito, L., S. Little, R. Langer, and M. Amiji. 2008. Poly(β-amino ester) and Cationic Phospholipid-Based Lipopolyplexes for Gene Delivery and Transfection in Human Aortic Endothelial and Smooth Muscle Cells. Biomacromolecules. 9:1179-1187. HAOSMC
2007
Srinivas, A., and A. Ramamurthi. 2007. Effects of gamma-irradiation on physical and biologic properties of crosslinked hyaluronan tissue engineering scaffolds. Tissue engineering. 13:447-459.
Wang, M., J. Zhang, L. Jiang, G. Spinetti, G. Pintus, R. Monticone, F. Kolodgie, R. Virmani, and E. Lakatta. 2007. Proinflammatory Profile Within the Grossly Normal Aged Human Aortic Wall. Hypertension, 50:219-227.
2006
Leik, C.E., E.J. Su, P. Nambi, D.L. Crandall, and D.A. Lawrence. 2006. Effect of pharmacologic plasminogen activator inhibitor-1 inhibition on cell motility and tumor angiogenesis. Journal of Thrombosis and Haemostasis. 4:2710-2715.
Scheller, A. 2006. Notch signaling pathway in human vascular smooth muscle cell differentiation. Dublin City University, M.Sc. dissertation.
2005
Gravel, S.-P., and M.J. Servant. 2005. Roles of an IκB Kinase-related Pathway in Human Cytomegalovirus-infected Vascular Smooth Muscle Cells: A MOLECULAR LINK IN PATHOGEN-INDUCED PROATHEROSCLEROTIC CONDITIONS. Journal of Biological Chemistry. 280:7477-7486.
Sottile, J., and J. Chandler. 2005. Fibronectin Matrix Turnover Occurs through a Caveolin-1–dependent Process. Molecular biology of the cell. 16:757-768.
Toyoshima, T., N. Nishi, H. Kusama, R. Kobayashi, and T. Itano. 2005. 36-kDa microfibril-associated glycoprotein (MAGP-36) is an elastin-binding protein increased in chick aortae during development and growth. Exp. cell research. 307:224-230.
2002
Sottile, J., and D.C. Hocking. 2002. Fibronectin Polymerization Regulates the Composition and Stability of Extracellular Matrix Fibrils and Cell-Matrix Adhesions. Molecular biology of the cell. 13:3546-3559.
1999
Wang, R., M. Xu, R. Marcel, G. Bouliane, and D.Z. Fisher. 1999. Selective neointimal gene transfer in an avian model of vascular injury. Atherosclerosis. 146:71-82.
1998
Leung, K.H., V. Pippalla, A. Kreutter, and M. Chandler. 1998. Functional effects of FGF-13 on human lung fibroblasts, dermal microvascular endothelial cells, and aortic smooth muscle cells. Biochem. & Biophys. Research Communic. 250:137-142.