5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Human astrocytes (HA) are derived from human cerebral cortex. Astrocytes are the most abundant cells in the central nervous system where they perform many functions, such as providing mechanical support and nutrients to neurons and removal of wastes from neurons; providing signaling to endothelial cells; regulating neurogenesis and controlling synaptic function. As the recognition of the importance of astrocytes in nervous system is increasing, HA serve as a useful in vitro model for exploring the diversity of astrocytes functions.
Human Astrocytes from Cell Applications, Inc. have been used to:
- Investigate aberrant gene expression.
- Reveal that CBX6 overexpression inhibits proliferation of glyoblastoma cells and
- Demonstrate the role of epigenetics in glyioblastoma malignancy.
- Investigate choline uptake system in normal and glioblastoma cells with the hope to improve tomography imaging in glioblastoma patients
Characterization: Stained positive for GFAP
Normal healthy Human brain
|No bacteria, yeast, fungi, mycoplasma, virus|
|Positive for GFAP|
|Attach, spread, proliferate in Growth Medium|
500,000 HA (2nd passage) frozen in Basal Medium w/10% FBS, 10% DMSO
Cryovial frozen HA (882A-05), Grwoth Medium (821-500), Subculture Rgnt Kit (090K)
Shipped in Gr Med, 3rd psg (flasks or plates)
At least 10
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved HA, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 882AK-05f||Price: $832.00|
|Cryopreserved HA, Fetal: Frozen HA (5x10^5)||Size: 1 Cryovial||CAT.#: 882A-05f||Price: $679.00|
|Proliferating HA, Fetal: Actively growing, dividing cells, in medium||Size: T-25 Flask||CAT.#: 883A-25f||Price: $679.00|
|Proliferating HA, Fetal: Actively growing, dividing cells, in medium||Size: 6 Well||CAT.#: 883A-6Wf||Price: $869.00|
|Proliferating HA, Fetal: Actively growing, dividing cells, in medium||Size: T-75 Flask||CAT.#: 883A-75f||Price: $869.00|
|Proliferating HA, Fetal: Actively growing, dividing cells, in medium||Size: 96 Well||CAT.#: 883A-96Wf||Price: $989.00|
|HA Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: 820-500||Price: $67.00|
|HA Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: 821-500||Price: $109.00|
|HA Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: 821K-500||Price: $118.00|
|HA Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 50 ml||CAT.#: 821-GS||Price: $51.00|
Extended Family Products
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|HA RNA, Fetal: Total RNA prepared from Human Astrocytes, fetal||Size: 10 ug||CAT.#: 882A-R10f||Price: $409.00|
|HA RNA, Fetal: Total RNA prepared from Human Astrocytes, fetal||Size: 25 ug||CAT.#: 882A-R25f||Price: $818.00|
|Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal||Size: 50 ug||CAT.#: 1F01-50||Price: $139.00|
|Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal||Size: 250 ug||CAT.#: 1F01-250||Price: $522.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
Wang, P., F. Zhu and K. Konstantopoulos. 2012. The Antagonistic Actions of Endogenous Interleukin-1β and 15-Deoxy-Δ12,14-prostaglandin J2 Regulate the Temporal Synthesis of Matrix Metalloproteinase-9 in Sheared Chondrocytes.
Taguchi, C., M. Inazu, I. Saiki, M. Yara, N. Hara, T. Yamanaka, and H. Uchino. 2014. Functional analysis of [methyl-3H]choline uptake in glioblastoma cells: influence of anti-cancer and central nervous system drugs. Biochemical pharmacology. 88:303-312.
Li, G., C. Warden, Z. Zou, J. Neman, J.S. Krueger, A. Jain, R. Jandial, and M. Chen. 2013. Altered Expression of Polycomb Group Genes in Glioblastoma Multiforme. PloS one. 8:e80970.