MSDS Cryopreserved Cells
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Description
Human Brachiocephalic Artery Endothelial Cells (HBcAEC) along with human aortic (HAOEC), carotid artery (HCtAEC), coronary artery (HCAEC) and subclavian artery (HScAEC), all from Cell Applications, Inc., have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response. The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors. To eliminate the donor-to-donor variability, the scientists took advantage of the great variety of primary cells offered by Cell Applications, including the option of ordering a panel of endothelial cells obtained from different vascular beds of the same donor!
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
Human Brachiocephalic Artery Endothelial Cells :HBcAEC (L). HBcAEC immunolabeled for vWF (green); nuclei are counterstained with PI (red) (R).
Details
Tissue | Normal health human brachiocephalic artery |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Factor VIII-related Ag, DiI-Ac-LDL uptake |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HBcAEC (2nd psg) frozen in Basal Medium w/10% FBS, 10% DMSO |
Kit | Cryovial frozen HBcAEC (3012-05a), Growth Medium (212-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, 3rd psg (flasks or plates) |
Doublings | At least 12 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Brachiocephalic Artery Endothelial Cells Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 3012K-05a | Price: $1,014.00 | |
| Cryopreserved Brachiocephalic Artery Endothelial Cells (HBcAEC), adult: Frozen HBcAEC (5x10^5) | Size: 1 Cryovial | CAT.#: 3012-05a | Price: $825.00 | |
| Proliferating Brachiocephalic Artery Endothelial Cells (HBcAEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 3013-25a | Price: $825.00 | |
| Proliferating Brachiocephalic Artery Endothelial Cells (HBcAEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 3013-75a | Price: $1,015.00 | |
| Proliferating Brachiocephalic Artery Endothelial Cells (HBcAEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 3013-24Wa | Price: $1,015.00 | |
| Proliferating Brachiocephalic Artery Endothelial Cells (HBcAEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 3013-96Wa | Price: $1,135.00 | |
| Cryopreserved Brachiocephalic Artery Endothelial Cells, Type 2 Diabetes (HBcAEC-T2D), adult: Frozen HBcAEC-T2D from donor with Type 2 Diabetes (5x10^5) | Size: 1 Cryovial | CAT.#: 3012T2D-05a | Price: $925.00 | |
| Cryopreserved Human Brachiocephalic Artery Endothelial Cells, Type 2 Diabetes Total Kit, adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 3012T2DK-05a | Price: $1,114.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Meso Endo Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 212-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red | Size: 500 ml | CAT.#: 212PR-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics | Size: 500 ml | CAT.#: 212A-500 | Price: $143.00 | |
| Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 212K-500 | Price: $147.00 | |
| Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red | Size: yields 500 ml | CAT.#: 212KPR-500 | Price: $150.00 | |
| Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 212-GS | Price: $81.00 | |
| Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: 212F-500 | Price: $156.00 | |
| Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS | Size: 5 ml | CAT.#: 212F-GS | Price: $88.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody | Size: 100 ul | CAT.#: CA1080 | Price: $302.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Brachiocephalic Artery Endothelial Cell RNA (HBcAEC RNA), Adult: Total RNA prepared from Human Brachiocephalic Artery Endothelial Cells, adult | Size: 10 ug | CAT.#: 3012-R10a | Price: $465.00 | |
| Brachiocephalic Artery Endothelial Cell RNA (HBcAEC RNA), Adult: Total RNA prepared from Human Brachiocephalic Artery Endothelial Cells, adult | Size: 25 ug | CAT.#: 3012-R25a | Price: $930.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2013
Scott, D.W., M.O. Vallejo, and R.P. Patel. 2013. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. Journal of the American Heart Association, 2:e000263-e000263.