MSDS 140-500
MSDS 510-500
MSDS 511-500
MSDS 511A-500
MSDS 511B-500
MSDS 511BHI-500
MSDS 511D-250
MSDS 511-GS
MSDS 511K-500
MSDS 511T3-500

Description

Medium carefully optimized for the characteristics and requirements unique to Human Bronchial and Tracheal Epithelial Cells.  Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.  As with the majority of our epithelial cell media, this product contains no serum.
 
Use with:
EpiVita Basal Medium (See image in "Details" tab), used in conjunction with Human Bronchial/Tracheal Epithelial Cell Growth Supplement, provides excellent culture conditions for HBEpC and HTEpC.  This robust and versatile basal medium is animal origin-free, and contains essential & non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.  Available only from Cell Applications, top-performing EpiVita Basal Medium (plus supplement), outperforms competitor formulations by a wide margin. Manufactured in a GMP certified facility, EpiVita is an ideal choice for fundamental life science studies, pharmaceutical drug discovery & toxicity assay, and translational research aimed at taking discoveries from the bench to the clinic.
 
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
(Click to Enlarge) Human Bronchial/Tracheal Epithelial Cell Media is quality-tested for sterility, with no bacteria, yeast or fungi contamination.  It also has correct pH, osmolality, passes low endotoxin criteria and promotes cell attachment, proliferation and spreading.

 

Details

EpiVita Basal Med w/ Growth Supp Increases HBEpC Lifespan
A. Population Doublings (L) & Cumulative PDs (R) for adult HBEpC grown in EpiVita Basal Medium with added Bronchial/Tracheal Epithelial Growth Supplement.  B. Growth Density in standard Bronchial/Tracheal Epithelial Cell Growth Medium.
 

Products

Product Size CAT.# Price Quantity
HBEpC/HTEpC Growth Medium: All-in-one ready-to-use. Cat# 511-500 fully supports cell growth on its own. Retinoic acid (RA) is a optional component; one vial of RA goes into 500ml media. Some researchers may elect to include RA if studies include cell differentiation or pseudo-stratification. Size: 500 ml CAT.#: 511-500 Price: $126.00
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HBEpC/HTEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Kit CAT.#: 511K-500 Price: $125.00
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HBEpC/HTEpC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 511-GS Price: $62.00
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HBEpC/HTEpC Basal Medium: Without growth supplement (GS).  Add GS before use. Size: 500 ml CAT.#: 510-500 Price: $62.00
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HBEpC/HTEpC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 511D-250 Price: $129.00
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HBEpC/HTEpC Growth Medium wo BPE: Growth medium without BPE Size: 500 ml CAT.#: 511B-500 Price: $125.00
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HBEpC/HTEpC Growth Medium wo BPE, Hydrocortisone, Insulin: Growth medium without BPE, hydrocortisone and insulin Size: 500 ml CAT.#: 511BHI-500 Price: $125.00
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HBEpC/HTEpC Growth Medium wo T3: Growth medium without Tri-iodothyronine (T3) Size: 500 ml CAT.#: 511T3-500 Price: $125.00
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HBEpC/HTEpC Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 511A-500 Price: $125.00
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Related Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $404.00
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Resources/Documents

Publications

2016
Chiu, W., Y. Lee, Y. Su, Y. Wang, C. Tsai, Y. Hou, C. Wang, Y. Huang, C. Huang, S. Chou, P. Hsieh and S. Yuan. 2016. The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.  PLoS ONE, dx.doi.org/10.1371/journal.pone.0166453
Liu, Z., X. Chen, Q. Wu, J. Song, L. Wang and G. Li. 2016. miR-125b inhibits goblet cell differentiation in allergic airway inflammation by targeting SPDEF. Eur J Pharmacol, doi:10.1016/j.ejphar.2016.04.044.
Mungunsukh, O., Y. Lee, D. Bottaro and R. Day. 2016. The Hepatocyte Growth Factor Isoform NK2 Activates Motogenesis and Survival but not Proliferation due to Lack of Akt Activation. Cellular Signaling, doi:10.1016/j.cellsig.2016.05.012.
Perez, D. and H. Chen. 2016. Methods and Compositions for in vivo Immune Stimulation and Antigen Production. Patent Application US 20160022807 A1.
2015
Li, J., J. Guan X. Long and X. Xiang. 2015. Endothelin-1 Upregulates the Expression of High Mobility Group Box 1 in Human Bronchial Epithelial Cells. Pharmacology, 96:144-150.
Tseng, R., J. Chang, J. Chen, W. Huang, Y. Tang, I. Kuo, J. Yan, W. Lai and Y. Wang. 2015. Deregulation of SLIT2-Mediated Cdc42 Activity Is Associated with Esophageal Cancer Metastasis and Poor Prognosis. J Thoracic Oncol, 10:189-198.
2014
Voiles, L., D. Lewis, L. Han, I. Lupov, T. Lin, M. Robertson, I. Petrache and H. Chang. 2014. Overexpression of type VI collagen in neoplastic lung tissues. Oncology Reports, 32:1897-1904.
2011
Rosler, E., E. Kunkel, S. Privat, J. Melrose, M. Fischer, and E. Berg. 2011. Biological dataset profiling of asthma and atopy. Patent US 8019551 B2.
2010
Lung, J., K.-J. Liu, J.-Y. Chang, S.-J. Leu, and N.-Y. Shih. 2010. MBP-1 is efficiently encoded by an alternative transcript of the ENO1 gene but post-translationally regulated by proteasome-dependent protein turnover. FEBS Journal. 277:4308-4321.
Maier, K.G., X. Han, B. Sadowitz, K.L. Gentile, F.A. Middleton, and V. Gahtan. 2010. Thrombospondin-1: a proatherosclerotic protein augmented by hyperglycemia. Journal of Vascular Surgery. 51:1238-1247.
Mungunsukh, O., A.J. Griffin, Y.H. Lee, and R.M. Day. 2010. Bleomycin induces the extrinsic apoptotic pathway in pulmonary endothelial cells. American Journal of Physiology - Lung Cellular and Molecular Physiology. 298:L696-L703.
Shen, C., C. Wang, J. Liao, T. Hsu, S. Kuo and H. Su. 2010. The infection of primary avian tracheal epithelial cells with infectious bronchitis virus. Veterinary Research, 41:06.