MSDS Cryopreserved Cells
Instructions HCtAEC Normal
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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Description

Human Carotid Artery Endothelial Cells (HCtAEC) have been used to elucidate
  • Molecular mechanisms of cerebral aneurism caused by haemodynamic (sheer) stress and inflammation
  • PGE2-EP2 and NF-κB signaling
  • Links between inflammation and neovascularization
  • Cell migration, wound healing and tube formation responses
  • Inhibition of angiogenesis
  • CD40-CD154 signaling between endothelial cells and T cells
  • EC stress responses
  • That blood vessels from different tissues are highly heterogeneous and interact differently with leukocytes during the inflammation response.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
Human Carotid Artery Endothelial Cells: HCtAEC (L)HCtAEC immunolabeled for vWF (green) and nuclei visualized with PI (red) (R).

Details

Tissue
Normal healthy human carotid artery
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag, DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HCtAEC (2nd psg) Frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HCtAEC (3014-05a), Growth Medium (212-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Tsfr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HCtAEC Normal

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HCtAEC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 3014K-05a Price: $898.24
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Cryopreserved HCtAEC, adult: Frozen HCtAEC (5x10^5) Size: 1 Cryovial CAT.#: 3014-05a Price: $747.99
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Cryopreserved HCtAEC-AS Total Kit: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents Size: 1 Kit CAT.#: 3014ASK-05a Price: $971.44
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Cryopreserved HCtAEC-AS, adult: Frozen HCtAEC-AS from donor with Asthma (5x10^5) Size: 1 Cryovial CAT.#: 3014AS-05a Price: $823.08
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Proliferating HCtAEC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 3015-25a Price: $747.99
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Proliferating HCtAEC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 3015-75a Price: $952.48
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Proliferating HCtAEC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 3015-6Wa Price: $952.48
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Proliferating HCtAEC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 3015-96Wa Price: $1,081.63
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Related Products

Product Size CAT.# Price Quantity
Human MesoEndo Cell Growth Medium: Growth medium without antibiotics Size: 500 ml CAT.#: 212A-500 Price: $123.77
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Human MesoEndo Cell Growth Medium: Growth Medium without FBS Size: 500 ml CAT.#: 212F-500 Price: $136.33
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Human MesoEndo Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 212-500 Price: $118.39
-+
Human MesoEndo Cell Growth Medium: Growth medium without phenol red Size: 500 ml CAT.#: 212PR-500 Price: $118.39
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Human MesoEndo Cell Growth Medium Kit: Growth medium kit without phenol red Size: yields 500 ml CAT.#: 212KPR-500 Price: $130.23
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Human MesoEndo Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 212K-500 Price: $118.39
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Human MesoEndo Cell Growth Supplement: Growth Supplement without FBS Size: 5 ml CAT.#: 212F-GS Price: $68.00
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Human MesoEndo Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 212-GS Price: $62.42
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Anti-VEGF: Polyclonal Vascular Endothelial Growth Factor Antibody Size: 100 ul CAT.#: CA1080 Price: $302.38
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Anti-VEGF-C: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.38
-+
Anti-VEGFR1: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.13
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
-+
Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $430.50
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $53.76
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E-Selectin ELISA Kit, Human: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $586.56
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HCtAEC RNA: Total RNA prepared from Human Carotid Artery Endothelial Cells, adult Size: 10 ug CAT.#: 3014-R10a Price: $408.98
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HCtAEC RNA: Total RNA prepared from Human Carotid Artery Endothelial Cells, adult Size: 25 ug CAT.#: 3014-R25a Price: $817.95
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $138.57
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $521.98
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ICAM-1 ELISA Kit, Human: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.31
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $193.73
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.31
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,174.94
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P-Selectin ELISA Kit, Human: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $516.60
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $193.73
-+
VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.31
-+
VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,089.75
-+
VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.10
-+
VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $532.74
-+
VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,498.73
-+
VEGF-C ELISA Kit, Human: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.18
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2017

Pott, G. B., Tsurudome, M., Bui, J., Banfield, C., Hourieh, S., Pratap, H., & Goalstone, M. L. 2017. VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Endothelial Cells. Medical Research Archives. Volume 5, issue 7.

2015
Chami, B., N. Barrie, X. Cai, X. Wang, M. Paul, R. Morton-Chandra,m A. Sharland, J. Dennis, S. Freedman and P. Witting. 2015. Serum Amyloid A Receptor Blockade and Incorporation into High-Density Lipoprotein Modulates Its Pro-Inflammatory and Pro-Thrombotic Activities on Vascular Endothelial Cells. Int J Mol Sci 2015:11101-11124.
2014
Morrison, D., J. Aitken, M. deJonge, F. Issa, H. Harris, and L. Rendina. 2014. Synthesis and Biological Evaluation of a Class of Mitochondrially‐Targeted Gadolinium (III) Agents. Chemistry – A European Journal, 20:16602-16612.
2013
Cai, X., S.B. Freedman, and P.K. Witting. 2013. Serum amyloid A stimulates cultured endothelial cells to migrate and proliferate: inhibition by the multikinase inhibitor BIBF1120. Clinical and Experimental Pharmacology and Physiology. 40:662-670.
Scott, D.W., M.O. Vallejo, and R.P. Patel. 2013. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. Journal of the American Heart Association. 2:e000263-e000263.
2011
Aoki, T., M. Nishimura, T. Matsuoka, K. Yamamoto, T. Furuyashiki, H. Kataoka, S. Kitaoka, R. Ishibashi, A. Ishibazawa, and S. Miyamoto. 2011. PGE2‐EP2 signalling in endothelium is activated by haemodynamic stress and induces cerebral aneurysm through an amplifying loop via NF‐κB. British journal of pharmacology. 163:1237-1249.
2008
Pluvinet, R., R. Olivar, J. Krupinski, I. Herrero-Fresneda, A. Luque, J. Torras, J.M. Cruzado, J.M. Grinyó, L. Sumoy, and J.M. Aran. 2008. CD40: an upstream master switch for endothelial cell activation uncovered by RNAi-coupled transcriptional profiling. Blood. 112:3624-3637.