MSDS Cryopreserved Cells
Instructions HCtAEC Normal
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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Description
Human Carotid Artery Endothelial Cells (HCtAEC) have been used to elucidate
- Molecular mechanisms of cerebral aneurism caused by haemodynamic (sheer) stress and inflammation
- PGE2-EP2 and NF-κB signaling
- Links between inflammation and neovascularization
- Cell migration, wound healing and tube formation responses
- Inhibition of angiogenesis
- CD40-CD154 signaling between endothelial cells and T cells
- EC stress responses
- That blood vessels from different tissues are highly heterogeneous and interact differently with leukocytes during the inflammation response.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
Human Carotid Artery Endothelial Cells: HCtAEC (L). HCtAEC immunolabeled for vWF (green) and nuclei visualized with PI (red) (R).
Details
Tissue | Normal healthy human carotid artery |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Factor VIII-related Ag, DiI-Ac-LDL uptake |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HCtAEC (2nd psg) Frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HCtAEC (3014-05a), Growth Medium (212-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Carotid Artery Endothleial Cell Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 3014K-05a | Price: $1,014.00 | |
| Cryopreserved Carotid Artery Endothelial Cells (HCtAEC), adult: Frozen HCtAEC (5x10^5) | Size: 1 Cryovial | CAT.#: 3014-05a | Price: $825.00 | |
| Cryopreserved Carotid Artery Endothelial Cells, Asthma (HCtAEC-AS), adult: Frozen HCtAEC-AS from donor with Asthma (5x10^5) | Size: 1 Cryovial | CAT.#: 3014AS-05a | Price: $925.00 | |
| Cryopreserved Human Carotid Artery Endothleial Cell with Asthma Total Kit, adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents | Size: 1 Kit | CAT.#: 3014ASK-05a | Price: $1,114.00 | |
| Proliferating Carotid Artery Endothelial Cells (HCtAEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 3015-25a | Price: $825.00 | |
| Proliferating Carotid Artery Endothelial Cells (HCtAEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 3015-75a | Price: $1,015.00 | |
| Proliferating Carotid Artery Endothelial Cells (HCtAEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 3015-24Wa | Price: $1,015.00 | |
| Proliferating Carotid Artery Endothelial Cells (HCtAEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 3015-96Wa | Price: $1,135.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Meso Endo Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 212-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 212K-500 | Price: $147.00 | |
| Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics | Size: 500 ml | CAT.#: 212A-500 | Price: $143.00 | |
| Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red | Size: 500 ml | CAT.#: 212PR-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red | Size: yields 500 ml | CAT.#: 212KPR-500 | Price: $150.00 | |
| Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 212-GS | Price: $81.00 | |
| Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: 212F-500 | Price: $156.00 | |
| Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS | Size: 5 ml | CAT.#: 212F-GS | Price: $88.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody | Size: 100 ul | CAT.#: CA1080 | Price: $302.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Carotid Artery Endothelial Cell RNA (HCtAEC RNA), Adult: Total RNA prepared from Human Carotid Artery Endothelial Cells, adult | Size: 10 ug | CAT.#: 3014-R10a | Price: $465.00 | |
| Carotid Artery Endothelial Cell RNA (HCtAEC RNA), Adult: Total RNA prepared from Human Carotid Artery Endothelial Cells, adult | Size: 25 ug | CAT.#: 3014-R25a | Price: $930.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2017
Pott, G. B., Tsurudome, M., Bui, J., Banfield, C., Hourieh, S., Pratap, H., & Goalstone, M. L. 2017. VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Endothelial Cells. Medical Research Archives. Volume 5, issue 7.
2015
Chami, B., N. Barrie, X. Cai, X. Wang, M. Paul, R. Morton-Chandra,m A. Sharland, J. Dennis, S. Freedman and P. Witting. 2015. Serum Amyloid A Receptor Blockade and Incorporation into High-Density Lipoprotein Modulates Its Pro-Inflammatory and Pro-Thrombotic Activities on Vascular Endothelial Cells. Int J Mol Sci 2015:11101-11124.
2014
Morrison, D., J. Aitken, M. deJonge, F. Issa, H. Harris, and L. Rendina. 2014. Synthesis and Biological Evaluation of a Class of Mitochondrially‐Targeted Gadolinium (III) Agents. Chemistry – A European Journal, 20:16602-16612.
2013
Cai, X., S.B. Freedman, and P.K. Witting. 2013. Serum amyloid A stimulates cultured endothelial cells to migrate and proliferate: inhibition by the multikinase inhibitor BIBF1120. Clinical and Experimental Pharmacology and Physiology. 40:662-670.
Scott, D.W., M.O. Vallejo, and R.P. Patel. 2013. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. Journal of the American Heart Association. 2:e000263-e000263.
2011
Aoki, T., M. Nishimura, T. Matsuoka, K. Yamamoto, T. Furuyashiki, H. Kataoka, S. Kitaoka, R. Ishibashi, A. Ishibazawa, and S. Miyamoto. 2011. PGE2‐EP2 signalling in endothelium is activated by haemodynamic stress and induces cerebral aneurysm through an amplifying loop via NF‐κB. British journal of pharmacology. 163:1237-1249.
2008
Pluvinet, R., R. Olivar, J. Krupinski, I. Herrero-Fresneda, A. Luque, J. Torras, J.M. Cruzado, J.M. Grinyó, L. Sumoy, and J.M. Aran. 2008. CD40: an upstream master switch for endothelial cell activation uncovered by RNAi-coupled transcriptional profiling. Blood. 112:3624-3637.