Specialized media, with ingredients and supplements optimized for the unique requirements of Human Chondrocytes (HC), providing optimal culture conditions, cell health, data integrity and reproducibility.
- Human Chondrocytes - Osteoarthritis: HC-OA
- Human Chondrocytes - Re-Differentiated: RD-HC
- Human Chondrocytes - Rheumatoid Arthritis: HC-RA
- Human Chondrocytes: HC
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions. The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Chondrogenesis Kit (Cat. No. 072K)
|Kit Component||Cat. No.||Storage|
|Sodium Alginate Solution||072-25||25C|
|Sodium Chloride Solution||074-100||25C|
|Calcium Chloride Solution||071-100||25C|
|Chondrocyte Differentiation Medium||411D-250||4C|
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
Sodium Chloride Solution: NaCl
Cat. No. 074-100 / 100 ml
Sterile filtered 0.155 M saline solution, ready-to-use
Calcium Chloride Solution: CaCl2
Cat. No. 071-100 / 100 ml
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
Chondrocyte Differentiation Medium
Cat. No. 411D-250
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
- Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
- Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
- Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
- Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
- Mix at room temperature for 30 minutes
- Apply gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
|HC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: 410-500||Price: $54.00|
|HC Basal Medium wo Phenol Red: Basal medium without phenol red||Size: 500 ml||CAT.#: 410PR-500||Price: $68.00|
|HC Differentiation Medium: Promotes cells to change from one type to another, more specialized||Size: 250 ml||CAT.#: 411D-250||Price: $96.00|
|HC Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: 411-500||Price: $122.00|
|HC Growth Medium wo Antibiotics: Growth medium without antibiotics||Size: 500 ml||CAT.#: 411A-500||Price: $118.00|
|HC Growth Medium wo FBS: Growth medium without FBS||Size: 500 ml||CAT.#: 411F-500||Price: $118.00|
|HC Growth Medium wo Phenol Red: Growth medium without phenol red||Size: 500 ml||CAT.#: 411PR-500||Price: $118.00|
|HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: 411K-500||Price: $111.00|
|HC Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red||Size: Yields 500 ml||CAT.#: 411KPR-500||Price: $122.00|
|HC Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 50 ml||CAT.#: 411-GS||Price: $57.00|
|HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red||Size: 250 ml||CAT.#: 411DPR-250||Price: $111.00|
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|Chondrocyte Differentiation Kit: Used to re-differentiate chondrocytes, that have been grown in monolayers, by encapsulation in alginate beads.||Size: 1 Kit||CAT.#: 072K||Price: $199.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
Extended Family Products
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF402K||Price: $431.00|
|Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF402KS||Price: $54.00|
Dou, P., R. Hu, W. Zhu, Q. Tang, D Li, H. Li and W. Wang. 2017. Long non-coding RNA HOTAIR promotes expression of ADAMTS-5 in human osteoarthritic articular chondrocytes. Die Pharmazie, 72:113-117.
Dua, R., K. Comella, R. Butler, G. Castellanos, B. Brazille, A. Claude, A. Agarwal, J. Liao and S. Ramaswamy. 2016. Integration of Stem Cell to Chondrocyte-Derived Cartilage Matrix in Healthy and Osteoarthritic States in the Presence of Hydroxyapatite Nanoparticles. PLoS ONE, 11(2): e0149121.
Kim, D., J. Song, Y. Kang, S. Park, Y. Kim, S. Kwak, D. Lim, R. Park, C. Chun, S. Choe and E. Jin. 2016. Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function. J Mol Med, 94:1373-1384.
Kong, Y., W. Wang, C. Zhang, Y. Wu, Y. Liu and X. Zhou. 2016. Cannabinoid WIN‑55,212‑2 mesylate inhibits ADAMTS‑4 activity in human osteoarthritic articular chondrocytes by inhibiting expression of syndecan‑1. Molecular Medicine Reports, 13:4569-4576.
Uddin, S., B. Richbourgh, Y. Ding, A. Hettinghouse, D. Komatsu, Y. Qin, and C. Liu. 2016. Chondro-protective effects of low intensity pulsed ultrasound. Osteoarthritis and Cartilage, 24:1989-1998.
Barrett, C. and Y. Shi. 2015. Cartilage mosaic compositions and methods. Patent US 9186253 B2.
Kang, Y., s. Park, C. Ahn, J. Song, D. Kim and E. Jin. 2015. Beneficial reward-to-risk action of glucosamine during pathogenesis of osteoarthritis. EU J Med Res, 20:89.
Chuang, Y. W. Chang, K. Chen, C. Hong, P. Chang and H. Hsu. 2014. Lysophosphatidic Acid Enhanced the Angiogenic Capability of Human Chondrocytes by Regulating Gi/NF-kB-Dependent Angiogenic Factor Expression. PLoS ONE, dx.doi.org/10.1371/journal.pone.0095180.
Hashizume, M. and M. Mihara. 2014. Combination of High-Molecular-Weight Hyaluronic Acid and Cytokine Inhibitor Potently Inhibits Expression of Joint-Damage-Related Genes Induced By Synovial Fluid of RA Patients. J Arthritis, 3:1.
Rolfes, E., J. Ross, J. McGonigle, G. Opperman and S. Chudzik. 2014. Poly-α(1→4)glucopyranose-based matrices with hydrazide crosslinking. Patent US8790701 B2.
Song, J., C. Ahn, C. Chun and E. Jin. 2014. A long non-coding RNA, GAS5, plays a critical role in the regulation of miR-21 during osteoarthritis. J Orthopaedic Res, 32:1628-1635.
Wei, J., H. Tseng, E. Chen, C. Hung, Y. Liang, M. Sheu and C., Chen. 2012. Characterizations of Chondrocyte Attachment and Proliferation on Electrospun Biodegradable Scaffolds of PLLA and PBSA for Use in Cartilage Tissue Engineering. J Biomater Appl, 26:963-985.
Attur, M., J.S. Millman, M.N. Dave, H.E. Al-Mussawir, J. Patel, G. Palmer, and S.B. Abramson. 2011. Glatiramer acetate (GA), the immunomodulatory drug, inhibits inflammatory mediators and collagen degradation in osteoarthritis (OA) cartilage. Osteoarthritis and Cartilage. 19:1158-1164.
Lin, B. B. Cunningham, and P. Li. 2011. Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor. Patent US 7875434 B2.
Yang, L., A. Guo, and J. Gu. 2011. c-Jun N-terminal kinase and nuclear factor κB mediate nitric oxide-induced expression of matrix metalloproteinase-13. Int Orthop, 35:1261-1266.
Ek, S. 2010. Nanorough Alloy Substrate. Patent Application US 20100185294 A1.
Odani-Kawabata, N., M. Takai-Imamura, O. Katsuta, H. Nakamura, K. Nishioka, K. Funahashi, T. Matsubara, M. Sasano, and H. Aono. 2010. ARG098, a novel anti-human Fas antibody, suppresses synovial hyperplasia and prevents cartilage destruction in a severe combined immunodeficient-HuRAg mouse model. BMC musculoskeletal disorders. 11:221.
Burns, K., C. Yao, and T.J. Webster. 2009. Increased chondrocyte adhesion on nanotubular anodized titanium. Journal of Biomedical Materials Research Part A. 88A:561-568.
Hashizume, M., and M. Mihara. 2009. Desirable effect of combination therapy with high molecular weight hyaluronate and NSAIDs on MMP production. Osteoarthritis and Cartilage. 17:1513-1518.
Mahoney, E. 2009. MOLECULAR ALTERATIONS IN BONE DEVELOPMENT AND BONE TUMORIGENESIS. PhD Dissertation, Ohio State University.
Park, G., B. Ward, and K. Webster. 2009. Bioresorbable poly (lactic/glycolic acid) (PLGA) materials/scaffolds with modified surfaces to enhance chondrocyte adhesion and proliferation; for use in repair of articular cartilage. Patent US 7527803 B2.