Specialized media, with ingredients and supplements optimized for the unique requirements of Human Chondrocytes (HC), providing optimal culture conditions, cell health, data integrity and reproducibility.
- Human Chondrocytes - Osteoarthritis: HC-OA
- Human Chondrocytes - Re-Differentiated: RD-HC
- Human Chondrocytes - Rheumatoid Arthritis: HC-RA
- Human Chondrocytes: HC
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions. The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Chondrogenesis Kit (Cat. No. 072K)
|Kit Component||Cat. No.||Storage|
|Sodium Alginate Solution||072-25||25C|
|Sodium Chloride Solution||074-250||25C|
|Calcium Chloride Solution||071-250||25C|
|Chondrocyte Differentiation Medium||411D-250||4C|
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
Sodium Chloride Solution: NaCl
Cat. No. 074-250 / 250 ml
Sterile filtered 0.155 M saline solution, ready-to-use
Calcium Chloride Solution: CaCl2
Cat. No. 071-250 / 250 ml
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
Chondrocyte Differentiation Medium
Cat. No. 411D-250
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
- Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
- Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
- Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
- Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
- Mix at room temperature for 30 minutes
- Apply gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
|HC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: 410-500||Price: $54.00|
|HC Basal Medium wo Phenol Red: Basal medium without phenol red||Size: 500 ml||CAT.#: 410PR-500||Price: $68.00|
|HC Differentiation Medium: Promotes cells to change from one type to another, more specialized||Size: 250 ml||CAT.#: 411D-250||Price: $96.00|
|HC Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: 411-500||Price: $122.00|
|HC Growth Medium wo Antibiotics: Growth medium without antibiotics||Size: 500 ml||CAT.#: 411A-500||Price: $118.00|
|HC Growth Medium wo FBS: Growth medium without FBS||Size: 500 ml||CAT.#: 411F-500||Price: $118.00|
|HC Growth Medium wo Phenol Red: Growth medium without phenol red||Size: 500 ml||CAT.#: 411PR-500||Price: $118.00|
|HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: 411K-500||Price: $111.00|
|HC Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red||Size: Yields 500 ml||CAT.#: 411KPR-500||Price: $122.00|
|HC Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 50 ml||CAT.#: 411-GS||Price: $57.00|
|HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red||Size: 250 ml||CAT.#: 411DPR-250||Price: $111.00|
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|Chondrogenesis Kit: Encapsulization of Chondrocytes in Alginate Beads (See details tab for specifics)||Size: 1 Kit||CAT.#: 072K||Price: $199.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
Extended Family Products
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF402K||Price: $431.00|
|Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF402KS||Price: $54.00|
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