MSDS 410-500
MSDS 410PR-500
MSDS 411-500
MSDS 411A-500
MSDS 411D-500
MSDS 411F-500
MSDS 411K-500
MSDS 411KPR-500
MSDS 411PR-500


Specialized media, with ingredients and supplements optimized for the unique requirements of Human Chondrocytes (HC), providing optimal culture conditions, cell health, data integrity and reproducibility.
Use with:
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions.  The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Human Chondrocyte Media for use with cartilage-producing cells.


Chondrogenesis Kit (Cat. No. 072K)
Kit ComponentCat. No.Storage
Sodium Alginate Solution072-2525C
Sodium Chloride Solution074-10025C
Calcium Chloride Solution071-10025C
Depolymerization Solution073-5025C
Chondrocyte Differentiation Medium411D-2504C
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
Sodium Chloride Solution: NaCl
Cat. No. 074-100 / 100 ml
Sterile filtered 0.155 M saline solution, ready-to-use
Calcium Chloride Solution: CaCl2
Cat. No. 071-100 / 100 ml
Sterile, ready-to-use
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
Chondrocyte Differentiation Medium
Cat. No. 411D-250
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
  • Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
  • Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
  • Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
  • Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
  1. Remove Chondrocyte Differentiation Medium
  2. Wash the beads 2 times with 0.155 M NaCl
  3. Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
  4. Mix at room temperature for 30 minutes
  5. Apply gentle agitation until the beads have completely dissolved
  6. Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
  7. Wash the cells 2 times with 0.155 M NaCl


Product Size CAT.# Price Quantity
HC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 410-500 Price: $70.00
HC Basal Medium wo Phenol Red: Basal medium without phenol red Size: 500 ml CAT.#: 410PR-500 Price: $88.00
HC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 411D-250 Price: $110.00
HC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 411-500 Price: $140.00
HC Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 411A-500 Price: $136.00
HC Growth Medium wo FBS: Growth medium without FBS Size: 500 ml CAT.#: 411F-500 Price: $136.00
HC Growth Medium wo Phenol Red: Growth medium without phenol red Size: 500 ml CAT.#: 411PR-500 Price: $136.00
HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 411K-500 Price: $128.00
HC Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red Size: Yields 500 ml CAT.#: 411KPR-500 Price: $140.00
HC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 411-GS Price: $74.00
HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red Size: 250 ml CAT.#: 411DPR-250 Price: $128.00

Related Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
Chondrogenesis Kit: Used to re-differentiate chondrocytes, that have been grown in monolayers, by encapsulation in alginate beads. Size: 1 Kit CAT.#: 072K Price: $229.00
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $63.00

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00
Cytofect Chondrocyte Transfection Kit (175 x 24-Wells): 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $496.00
Cytofect Chondrocyte Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $62.00



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