MSDS 072K
MSDS 410-500
MSDS 410PR-500
MSDS 411-500
MSDS 411A-500
MSDS 411D-500
MSDS 411F-500
MSDS 411-GS
MSDS 411K-500
MSDS 411KPR-500
MSDS 411PR-500

Description

Specialized media, with ingredients and supplements optimized for the unique requirements of Human Chondrocytes (HC), providing optimal culture conditions, cell health, data integrity and reproducibility.
 
Use with:
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions.  The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
 
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Human Chondrocyte Media for use with cartilage-producing cells.

Details

Chondrogenesis Kit (Cat. No. 072K)
Kit ComponentCat. No.Storage
Sodium Alginate Solution072-2525C
Sodium Chloride Solution074-25025C
Calcium Chloride Solution071-25025C
Depolymerization Solution073-5025C
Chondrocyte Differentiation Medium411D-2504C
 
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
 
Sodium Chloride Solution: NaCl
Cat. No. 074-250 / 250 ml
Sterile filtered 0.155 M saline solution, ready-to-use
 
Calcium Chloride Solution: CaCl2
Cat. No. 071-250 / 250 ml
Sterile, ready-to-use
 
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
 
Chondrocyte Differentiation Medium
Cat. No. 411D-250
Ready-to-use
 
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
  • Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
  • Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
  • Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
  • Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
  1. Remove Chondrocyte Differentiation Medium
  2. Wash the beads 2 times with 0.155 M NaCl
  3. Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
  4. Mix at room temperature for 30 minutes
  5. Apply gentle agitation until the beads have completely dissolved
  6. Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
  7. Wash the cells 2 times with 0.155 M NaCl

Products

Product Size CAT.# Price Quantity
HC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 410-500 Price: $54.00
-+
HC Basal Medium wo Phenol Red: Basal medium without phenol red Size: 500 ml CAT.#: 410PR-500 Price: $68.00
-+
HC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 411D-250 Price: $96.00
-+
HC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 411-500 Price: $122.00
-+
HC Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 411A-500 Price: $118.00
-+
HC Growth Medium wo FBS: Growth medium without FBS Size: 500 ml CAT.#: 411F-500 Price: $118.00
-+
HC Growth Medium wo Phenol Red: Growth medium without phenol red Size: 500 ml CAT.#: 411PR-500 Price: $118.00
-+
HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 411K-500 Price: $111.00
-+
HC Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red Size: Yields 500 ml CAT.#: 411KPR-500 Price: $122.00
-+
HC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 411-GS Price: $57.00
-+
HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red Size: 250 ml CAT.#: 411DPR-250 Price: $111.00
-+

Related Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
-+
Chondrogenesis Kit: Encapsulization of Chondrocytes in Alginate Beads (See details tab for specifics) Size: 1 Kit CAT.#: 072K Price: $199.00
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $431.00
-+
Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $54.00
-+

Resources/Documents

Publications

2017
Dou, P., R. Hu, W. Zhu, Q. Tang, D Li, H. Li and W. Wang. 2017. Long non-coding RNA HOTAIR promotes expression of ADAMTS-5 in human osteoarthritic articular chondrocytes. Die Pharmazie, 72:113-117.
2016
Dua, R., K. Comella, R. Butler, G. Castellanos, B. Brazille, A. Claude, A. Agarwal, J. Liao and S. Ramaswamy. 2016. Integration of Stem Cell to Chondrocyte-Derived Cartilage Matrix in Healthy and Osteoarthritic States in the Presence of Hydroxyapatite NanoparticlesPLoS ONE, 11(2): e0149121.
Kim, D., J. Song, Y. Kang, S. Park, Y. Kim, S. Kwak, D. Lim, R. Park, C. Chun, S. Choe and E. Jin. 2016. Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function. J Mol Med, 94:1373-1384.
Kong, Y., W. Wang, C. Zhang, Y. Wu, Y. Liu and X. Zhou. 2016. Cannabinoid WIN‑55,212‑2 mesylate inhibits ADAMTS‑4 activity in human osteoarthritic articular chondrocytes by inhibiting expression of syndecan‑1. Molecular Medicine Reports, 13:4569-4576.
Uddin, S., B. Richbourgh, Y. Ding, A. Hettinghouse, D. Komatsu, Y. Qin, and C. Liu. 2016. Chondro-protective effects of low intensity pulsed ultrasound. Osteoarthritis and Cartilage, 24:1989-1998.
2015
Barrett, C. and Y. Shi. 2015. Cartilage mosaic compositions and methods. Patent US 9186253 B2.
Kang, Y., s. Park, C. Ahn, J. Song, D. Kim and E. Jin. 2015. Beneficial reward-to-risk action of glucosamine during pathogenesis of osteoarthritis. EU J Med Res, 20:89.
2014
Chuang, Y. W. Chang, K. Chen, C. Hong, P. Chang and H. Hsu. 2014. Lysophosphatidic Acid Enhanced the Angiogenic Capability of Human Chondrocytes by Regulating Gi/NF-kB-Dependent Angiogenic Factor Expression. PLoS ONE, dx.doi.org/10.1371/journal.pone.0095180.
Rolfes, E., J. Ross, J. McGonigle, G. Opperman and S. Chudzik. 2014. Poly-α(1→4)glucopyranose-based matrices with hydrazide crosslinking. Patent US8790701 B2.
Song, J., C. Ahn, C. Chun and E. Jin. 2014. A long non-coding RNA, GAS5, plays a critical role in the regulation of miR-21 during osteoarthritis. J Orthopaedic Res, 32:1628-1635.
2012

 

Wei, J., H. Tseng, E. Chen, C. Hung, Y. Liang, M. Sheu and C., Chen. 2012. Characterizations of Chondrocyte Attachment and Proliferation on Electrospun Biodegradable Scaffolds of PLLA and PBSA for Use in Cartilage Tissue Engineering. J Biomater Appl, 26:963-985.

 

2011
Attur, M., J.S. Millman, M.N. Dave, H.E. Al-Mussawir, J. Patel, G. Palmer, and S.B. Abramson. 2011. Glatiramer acetate (GA), the immunomodulatory drug, inhibits inflammatory mediators and collagen degradation in osteoarthritis (OA) cartilage. Osteoarthritis and Cartilage. 19:1158-1164.
Lin, B. B. Cunningham, and P. Li. 2011. Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor. Patent US 7875434 B2.
Yang, L., A. Guo, and J. Gu. 2011. c-Jun N-terminal kinase and nuclear factor κB mediate nitric oxide-induced expression of matrix metalloproteinase-13. Int Orthop, 35:1261-1266.
2010
Ek, S. 2010. Nanorough Alloy Substrate. Patent Application US 20100185294 A1.
Odani-Kawabata, N., M. Takai-Imamura, O. Katsuta, H. Nakamura, K. Nishioka, K. Funahashi, T. Matsubara, M. Sasano, and H. Aono. 2010. ARG098, a novel anti-human Fas antibody, suppresses synovial hyperplasia and prevents cartilage destruction in a severe combined immunodeficient-HuRAg mouse model. BMC musculoskeletal disorders. 11:221.
2009
Burns, K., C. Yao, and T.J. Webster. 2009. Increased chondrocyte adhesion on nanotubular anodized titanium. Journal of Biomedical Materials Research Part A. 88A:561-568.
Hashizume, M., and M. Mihara. 2009. Desirable effect of combination therapy with high molecular weight hyaluronate and NSAIDs on MMP production. Osteoarthritis and Cartilage. 17:1513-1518.
Mahoney, E. 2009. MOLECULAR ALTERATIONS IN BONE DEVELOPMENT AND BONE TUMORIGENESIS. PhD Dissertation, Ohio State University.
Park, G., B. Ward, and K. Webster. 2009. Bioresorbable poly (lactic/glycolic acid) (PLGA) materials/scaffolds with modified surfaces to enhance chondrocyte adhesion and proliferation; for use in repair of articular cartilage. Patent US 7527803 B2.