Instructions HC-RD
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Chondrocytes Re-Differentiated (HC-RD) have been encapsulated in an alginate bead suspension.  HC-RD exhibit spherical morphology and have regained their ability to produce the phenotypic markers Aggrecan and Type II Collagen.  RD-HC can be used in tissue engineering experiments aimed to repair cartilage defects and to study chondrocyte differentiation and the effects of Growth factors & Cytokines on the suppression and maintenance of differentiated cartilage phenotype.
Instructions to release HC-RD from Alginate Beads
  • Remove Chondrocyte Differentiation Medium
  • Wash the beads 2 times with 0.155 M NaCl
  • Add 3 volume of Depolymerization Solution per volume of packed alginate beads.
  • Mix at room temperature for 30 minutes with gentle agitation until the beads have completely dissolved
  • Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
  • Wash the cells 2 times with 0.155 M NaCl
Media Salts & Chemicals used to Release HC-RD from alginate beads:
  • Calcium Chloride Solution: Formulated to induce polymerization of Sodium Alginate.
  • Sodium Alginate:  Encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes
  • Depolymerization Solution (DS): Consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
(Click to Enlarge) Re-Differentiated Human Chondrocytes (HC-RD), encapsulated in an alginate bead suspension.

Details

Tissue
Normal healthy human articular cartilage
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
N/A
Kit
N/A
Proliferating
Shipped in Differentiation Medium (flasks)
Doublings
N/A
Note
Cells shipped 10 days after encapsulation.  Re-Differentiated Chondrocytes expected 3 weeks after encapsulation, retain phenotype at least 1 week after complete re-differentiation.
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
  • Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
  • Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
  • Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
  • Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
  1. Remove Chondrocyte Differentiation Medium
  2. Wash the beads 2 times with 0.155 M NaCl
  3. Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
  4. Mix at room temperature for 30 minutes
  5. Apply gentle agitation until the beads have completely dissolved
  6. Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
  7. Wash the cells 2 times with 0.155 M NaCl
Chondrogenesis Kit (Cat. No. 072K)
Kit ComponentCat. No.Storage
Sodium Alginate Solution
072-2525C
Sodium Chloride Solution074-25025C
Calcium Chloride Solution071-25025C
Depolymerization Solution073-5025C
Chondrocyte Differentiation Medium411D-2504C
 
Antibiotic Antimycotic Solution: AAS
Cat. No. 010-100 / 100 ml
10,000 units/ml Penicillin-G, 10,000 ug/ml Streptomycin Sulfate, and 25 ug/ml Amphotericin in normal salineSterile filtered solution, 100X, dilute before use
 
Calcium Chloride Solution: CaCl2
Cat. No. 071-100 / 100 ml
Sterile, ready-to-use
 
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
 
Hank’s Balanced Salt Solution:  HBSS

Cat. No. 062-100 / 100 ml

Sterile 1X solution, ready-to-use

 
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
 
Sodium Chloride Solution: NaCl
Cat. No. 074-100 / 100 ml
Sterile filtered 0.155 M saline solution, ready-to-use
Instructions HC-RD

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Proliferating HC-RD, adult: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: 403RD-25a Price: $909.43
-+
Proliferating HC-RD, adult: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: 403RD-75a Price: $1,113.92
-+
Proliferating HC-RD, fetal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: 403RD-25f Price: $866.38
-+
Proliferating HC-RD, fetal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: 403RD-75f Price: $1,070.87
-+

Related Products

Product Size CAT.# Price Quantity
Human Chondrocyte Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 410-500 Price: $53.81
-+
Human Chondrocyte Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 411D-250 Price: $95.79
-+
Human Chondrocyte Differentiation Medium: Promotes cells to change from one type to another, more specialized, without Phenol Red Size: 250 ml CAT.#: 411DPR-250 Price: $110.70
-+
Human Chondrocyte Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 411-500 Price: $102.24
-+
Human Chondrocyte Growth Medium: Growth medium without phenol red Size: 500 ml CAT.#: 411PR-500 Price: $118.39
-+
Human Chondrocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 411K-500 Price: $110.85
-+
Human Chondrocyte Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 411-GS Price: $57.04
-+

Extended Family Products

Product Size CAT.# Price Quantity
Anti-MMP-1: Mouse MMP-1 Antibody Size: 100 ul CAT.#: CP10422 Price: $302.38
-+
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
-+
Chondrogenesis Kit: Encapsulization of Chondrocytes in Alginate Beads (See details tab for specifics) Size: 1 Kit CAT.#: 072K Price: $199.00
-+
CTGF: Human Connective Tissue Growth Factor Size: 20 ug CAT.#: RP1060-20 Price: $193.73
-+
CTGF: Human Connective Tissue Growth Factor Size: 100 ug CAT.#: RP1060-100 Price: $484.31
-+
CTGF: Human Connective Tissue Growth Factor Size: 1000 ug CAT.#: RP1060-1000 Price: $4,089.75
-+
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
-+
Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
-+
Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $430.50
-+
Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $53.76
-+
HC RNA: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult Size: 10 ul CAT.#: 402RD-R10a Price: $452.03
-+
HC RNA: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult Size: 25 ul CAT.#: 402RD-R25a Price: $904.05
-+
HC RNA: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal Size: 10 ul CAT.#: 402RD-R10f Price: $452.03
-+
HC RNA: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal Size: 25 ul CAT.#: 402RD-R25f Price: $904.05
-+
MMP-1 ELISA Kit, Human: Human Matrix Metalloproteinase-1 ELISA Kit Size: 96 Wells CAT.#: CL0458 Price: $495.08
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Skeletal System Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now