Instructions HC-RA
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Chondrocytes Rheumatoid Arthritis (HC-RA) are derived from human articular cartilage of donors with rheumatoid arthritis (RA).  Rheumatoid arthritis is an inflammatory disease that is characterized by overproliferation of synovial cells and their infiltration into adjacent joint tissues, accompanied by gradual destruction of cartilage and bone erosion.  Normally, chondrocytes are responsible for producing and maintaining the extracellular matrix of cartilage, but in RA cartilage, the environment is changed significantly due to inflammation and the invasion of other cells.  Therefore, HC-RA can be a useful model to study the effects of those changes on chondrocyte biology.  In parallel with normal HC, HC-RA can be used to investigate the differences between normal and pathological joints and evaluate potential treatment strategies.
 
Characterization: Positive for aggrecan after differentiation

Human Chondrocytes-Rheumatoid Arthritis: HC-RA.  Isolated from a donor patient with joint disease.

Details

Tissue
Human articular cartilage, from donor w/ rheumatoid arthrits
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HC-RA (1st passage) frozen in Basal Medium w/10% FBS, 10% DMSO
Kit
Cryovial frozen HC-RA (402RA-05), Growth Medium (411-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HC-RA

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HC-RA, Adult: 5x10^5 Cells (from donor with Rheumatoid Arthritis, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 402RAK-05a Price: $913.00
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Cryopreserved HC-RA, Adult: Frozen HC from donor with Rheumotoid Arthritis (5x10^5) Size: 1 Cryovial CAT.#: 402RA-05a Price: $768.00
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Proliferating HC-RA, Adult: Actively growing, dividing cells from donor with Rheumatoid Arthritis, in medium Size: T-25 Flask CAT.#: 403RA-25a Price: $748.00
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Proliferating HC-RA, Adult: Actively growing, dividing cells from donor with Rheumatoid Arthritis, in medium Size: T-75 Flask CAT.#: 403RA-75a Price: $952.00
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Proliferating HC-RA, Adult: Actively growing, dividing cells from donor with Rheumatoid Arthritis, in medium Size: 6 well CAT.#: 403RA-6Wa Price: $877.00
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Proliferating HC-RA, Adult: Actively growing, dividing cells from donor with Rheumatoid Arthritis, in medium Size: 96 Well CAT.#: 403RA-96Wa Price: $1,006.00
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Related Products

Product Size CAT.# Price Quantity
HC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 410-500 Price: $54.00
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HC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 411D-250 Price: $96.00
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HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red Size: 250 ml CAT.#: 411DPR-250 Price: $111.00
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HC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 411-500 Price: $122.00
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HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 411K-500 Price: $111.00
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HC Growth Medium wo Phenol Red: Growth medium without phenol red Size: 500 ml CAT.#: 411PR-500 Price: $118.00
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HC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 411-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $431.00
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Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HC, Re-Differentiated RNA, Adult: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult Size: 10 ul CAT.#: 402RD-R10a Price: $452.00
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HC, Re-Differentiated RNA, Adult: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult Size: 25 ul CAT.#: 402RD-R25a Price: $904.00
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HC, Re-Differentiated RNA, Fetal: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal Size: 10 ul CAT.#: 402RD-R10f Price: $452.00
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HC, Re-Differentiated RNA, Fetal: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal Size: 25 ul CAT.#: 402RD-R25f Price: $904.00
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Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor Size: 20 ug CAT.#: RP1060-20 Price: $194.00
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Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor Size: 100 ug CAT.#: RP1060-100 Price: $484.00
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Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor Size: 1000 ug CAT.#: RP1060-1000 Price: $4,090.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Skeletal System Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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