MSDS Cryopreserved Cells
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Cell Apps Poster Primary Cells
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Instructions HCK
Description
Human Corneal Keratocytes (HCK) are primary cells derived from human eye. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
Human Corneal Keratocytes (HCK) cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. HCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
Human Corneal Keratocytes: HCK Top: Phase-contrast, Bottom Left: HCK immunostained for Vimentin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.
Details
| Tissue | Normal healthy human cornea |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HCK (632-05a), Growth Medium (6111-500), Subculture Rgnt Kit (090K) |
| Proliferating | Shipped in Tsfr Med, psg 1, flasks or plates |
| Doublings | At least 15 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Corneal Keratocytes Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 632K-05a | Price: $878.00 | |
| Cryopreserved Corneal Keratocytes Cells (HCK), adult: Frozen HCK (5x10^5) | Size: 1 Cryovial | CAT.#: 632-05a | Price: $715.00 | |
| Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 633-25a | Price: $715.00 | |
| Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 633-75a | Price: $905.00 | |
| Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 633-24Wa | Price: $905.00 | |
| Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 633-96Wa | Price: $1,025.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Corneal Keratocyte Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 6111-500 | Price: $110.00 | |
| Corneal Keratocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: 1 Kit | CAT.#: 6111K-500 | Price: $121.00 | |
| Corneal Keratocyte Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 6110-500 | Price: $72.00 | |
| Corneal Keratocyte Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 6111-GS | Price: $65.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 |
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