MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions HCK

Description

Human Corneal Keratocytes (HCK) are primary cells derived from human eye. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
 
Human Corneal Keratocytes (HCK) cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. HCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
 
Human Corneal Keratocytes: HCK Top: Phase-contrast, Bottom Left: HCK immunostained for Vimentin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.

Details

TissueNormal healthy human cornea
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen HCK (632-05a), Growth Medium (6111-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in Tsfr Med, psg 1, flasks or plates
DoublingsAt least 15
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 
Instructions HCK

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved HCK, Adult: Frozen HCK (5x10^5) Size: 1 Cryovial CAT.#: 632-05a Price: $679.00
-+
Cryopreserved HCK, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 632K-05a Price: $829.00
-+
Proliferating HCK, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 633-25a Price: $679.00
-+
Proliferating HCK, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 633-75a Price: $869.00
-+
Proliferating HCK, Adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 633-24Wa Price: $869.00
-+
Proliferating HCK, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 633-96Wa Price: $989.00
-+

Related Products

Product Size CAT.# Price Quantity
HCK Basal Medium : Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: 6110-500 Price: $65.00
-+
HCK Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 6111-500 Price: $105.00
-+
HCK Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: 1 Kit CAT.#: 6111K-500 Price: $115.00
-+
HCK Growth Medium Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 6111-GS Price: $50.00
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now