MSDS Cryopreserved Cells
Instructions HCASMC Normal
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Coronary Artery Smooth Muscle Cells (HCASMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HCASMC from Cell Applications, Inc. have been utilized in a number of research studies, for example, to:
  • Study signaling pathways regulating smooth muscle differentiation and chronic inflammation of arterial wall that leads to artherosclerosis
  • Demonstrate that STAT-1 and STAT-3 regulate VEGF production in smooth muscle cells by having opposing effects on HIF-1α expression
  • Examine the mechanisms of hypoxia and reoxigenation injuries in by demonstrating increased production of ROS and inflammatory cytokines, and further showing that DHA is not beneficial in this type of injuries
  • Investigate (by also using human Internal Thoracic Artery Smooth Muscle Cells obtained from Cell Applications, Inc.), the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli
  • Suggest the hypermethylation of SOCS3 gene as the connection between TNF-α and IGF-1 released in response to mechanical injury during coronary intervention, and the induction of cytokines leading to intimal hyperplasia and restenosis
  • Develop a novel VEGFR/MET-targeted inhibitor with improved antitumor efficacy and decreased toxicity
  • Investigate novel therapies and drug combinations to achieve optimal target selectivity
  • Generate elastic scaffolds for tissue engineering and novel treatment strategies to prevent stent restenosis by designing new materials, or drug therapies to preferentially inhibit smooth muscle cell growth
 Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Coronary Artery Smooth Muscle Cells: HCASMC are isolated from two major vessels that supply the heart with blood.  TOP: Phase-contrast microscopic image under green light (L), Differentiated HCASMC stain positive for smooth muscle cell-specific alpha-actin expression (R). BOTTOM: Phase contrast (L) and composite fluorescent image of cells (red), and nuclei (blue) counterstained with DNA-specific DAPI probe.

Details

Tissue
Normal healthy human coronary artery
QC
No bacteria, yeast, fungi, mycoplasma, virus 
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HCASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HCASMC (350-05a), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HCASMC Normal

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HCASMC Plaque, Adult: 5x10^5 Cells (Plaque, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 350qK-05a Price: $944.00
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Cryopreserved HCASMC Plaque, Adult: Frozen HCASMC (5x10^5) Size: 1 Cryovial CAT.#: 350q-05a Price: $794.00
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Cryopreserved HCASMC Pooled, Adult: Frozen HCASMC (5x10^5) Size: 1 Cryovial CAT.#: 350p-05a Price: $735.00
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Cryopreserved HCASMC Pooled, Adult: 5x10^5 Cells (Pooled, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 350pK-05a Price: $885.00
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Cryopreserved HCASMC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 350K-05a Price: $885.00
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Cryopreserved HCASMC, Adult: Frozen HCASMC (5x10^5) Size: 1 Cryovial CAT.#: 350-05a Price: $735.00
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Cryopreserved HCASMC-AS, Adult: Frozen HCASMC-AS from donor with Asthma (5x10^5) Size: 1 Cryovial CAT.#: 350AS-05a Price: $802.00
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Cryopreserved HCASMC-AS, Adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 350ASK-05a Price: $952.00
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Proliferating HCASMC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 351-25a Price: $748.00
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Proliferating HCASMC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 351-75a Price: $925.00
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Proliferating HCASMC, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 351-6Wa Price: $925.00
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Proliferating HCASMC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 351-96Wa Price: $1,058.00
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Related Products

Product Size CAT.# Price Quantity
HCASMC Conditioned Medium: HCASMC Conditioned Medium Size: 5 ml CAT.#: 350cm-005 Price: $48.00
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Human SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.00
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Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.00
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Human SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.00
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Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.00
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Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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HCASMC RNA, Adult: Total RNA prepared from Human Coronary Artery Smooth Muscle Cells, adult Size: 10 ug CAT.#: 350-R10a Price: $350.00
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HCASMC RNA, Adult: Total RNA prepared from Human Coronary Artery Smooth Muscle Cells, adult Size: 25 ug CAT.#: 350-R25a Price: $700.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $139.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $522.00
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Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $517.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $533.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $194.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,175.00
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Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

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Cell Apps Flyer Cardiovascular Cells

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5 Important Cell Culture Rules

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Cell Apps Poster Primary Cells

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Cell Applications Inc Brochure

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Publications

2017

Shah, F., Stepan, A.F., O'Mahony, A., Velichko, S., Folias, A.E., Houle, C., Shaffer, C.L., Marcek, J., Whritenour, J., Stanton, R. and Berg, E.L., 2017. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators.Cell Chemical Biology, 858-869.e5.

2015
Baskar, K., S. Sur, V. Selvaraj and D. Agrawal. 2015. Functional constituents of a local serotonergic system, intrinsic to the human coronary artery smooth muscle cellsMolec Biol Repts, 42:1295-1307.
Boosani, C., K. Dhar, and D. Agrawal. 2015. Down-regulation of hsa-miR-1264 contributes to DNMT1-mediated silencing of SOCS3Molec Biol Repts, DOI 10.1007/s11033-015-3882-x. 
Gupta, M., S. Lee, S. Crowder, X. Wang, L. Hofmeister, C. Nelson, L. Bellan, C. Duvall, and H. Sung. 2015. Oligoproline-Derived Nanocarrier for Dual Stimuli-Responsive Gene DeliveryJournal of Materials Chemistry B, DOI: 10.1039/C5TB00988J.
Han, Y., Y. Cho, R. Ayon, R. Guo, K. Youssef, M. Pan, A. Dai, J. Yuan and A. Makino. 2015. SGLT inhibitors attenuate NO-dependent vascular relaxation in the pulmonary artery but not in the coronary arteryAm J Physiol Lung Cell Mol Physiol, 309:L1027-L1036.
Simone, T., S. Higgins, J. Archambeault, C. Higgins, R. Ginnan, H. Singer, and P. Higgins. 2015. A small molecule PAI-1 functional inhibitor attenuates neointimal hyperplasia and vascular smooth muscle cell survival by promoting PAI-1 cleavage. Cellular Signalling, 27:923-933.
Yu, Y., s. Wise, P. Michael, D. Bax, G. Yuen, M. Hiob, G. Yeo, E. Filipe, L. Dunn, K. Chan, H. Hajian, D. Celermajer, A. Weiss and M. Ng. 2015. Characterization of Endothelial Progenitor Cell Interactions with Human TropoelastinPLoS ONE 10(6): e0131101.
2014
Kiyan, Y., S. Tkachuk, D. Hilfiker-Kleiner, H. Haller, B. Fuhrman, and I. Dumler. 2014. oxLDL induces inflammatory responses in vascular smooth muscle cells via urokinase receptor association with CD36 and TLR4. J. Mol. & Cell. Cardiol. 66:72-82.
Lange, M., T. Fujikawa, A. Koulova, S. Kang, M. Griffin, A. Lassaletta, A. Erat., E. Tobiash, C. Bianchi, N. Elmadhun, F. Selke, and A. Usheva. 2014. Arterial territory-specific phosphorylated retinoblastoma protein species and CDK2 promote differences in the vascular smooth muscle cell response to mitogens. Cell Cylcle, 13:315-323.
Lord, M., C. Chuang, J. Melrose, M. Davies, R. Iozzo and J. Whitelock. 2014. The role of vascular-derived perlecan in modulating cell adhesion, proliferation and growth factor signaling. Matrix Biol, 35:112-122.
2013
Dhar, K., K. Rakesh, D. Pankajakshan, and D.K. Agrawal. 2013. SOCS3 promotor hypermethylation and STAT3-NF-κB interaction downregulate SOCS3 expression in human coronary artery smooth muscle cells. Am. J. Physiology. 304:H776-H785.
Fujita, H., K. Miyadera, M. Kato, Y. Fujioka, H. Ochiiwa, J. Huang, K. Ito, Y. Aoyagi, T. Takenaka, T. Suzuki, S. Ito, A. Hashimoto, T. Suefuji, K. Egami, H. Kazuno, Y. Suda, K. Nishio, and K. Yonekura. 2013. The Novel VEGF Receptor/MET–Targeted Kinase Inhibitor TAS-115 Has Marked In Vivo Antitumor Properties and a Favorable Tolerability Profile. Molecular Cancer Therapeutics. 12:2685-2696.
Lange, M. 2013. Artery Specific Differences in Cell Cycle Regulation are Associated with Serum Induced Proliferation of Vascular Smooth Muscle Cells. University of Applied Science Bonn-Rhine-Sieg, MSc dissertation.
Mociornita, A. 2013. The Role of Human Leukocyte Antigen-G in Cardiac Allograft Vasculopathy. University of Toronto, MSc dissertation.
Wu-Wong, J.R., M. Nakane, Y.-W. Chen, and W. Qiang. 2013. Different Effects of Calcidiol and Calcitriol on Regulating Vitamin D Receptor Target Gene Expression in Human Vascular Smooth Muscle Cells. J. CARDIOVASCULAR DISEASE. 1:15-20.
2012
Albasanz-Puig, A., J. Murray, M. Namekata, and E.S. Wijelath. 2012. Opposing roles of STAT-1 and STAT-3 in regulating vascular endothelial growth factor expression in vascular smooth muscle cells. Biochem. & Biophys. Res. Comm. 428:179-184.
Crowder, S.W., M.K. Gupta, L.H. Hofmeister, A.L. Zachman, and H.-J. Sung. 2012. Modular polymer design to regulate phenotype and oxidative response of human coronary artery cells for potential stent coating applications. Acta Biomaterialia. 8:559-569.
Feng, G.-M., J.-H. Chen, C.-I. Lin, and J.-M. Yang. 2012. Effect of docosahexaenoic acid on hypoxia/reoxygenation injury in human coronary arterial smooth muscle cells. Eur J Nutr. 51:987-995.
Jemy, J. 2012. Does Human Leukocyte Antigen-G (HLA-G) Play a Role in Immunte Modulation and Vasculopathy in Heart Transplantation?  Masters Thesis, U Toronto.
Khachigian, L. 2012. Vascular therapeutics. Patent US 8242090 B2.
NivisonSmith, L., and A.S. Weiss. 2012. Alignment of human vascular smooth muscle cells on parallel electrospun synthetic elastin fibers. Journal of Biomedical Materials Research Part A. 100:155-161.
2010
Nivison-Smith, L., J. Rnjak, and A.S. Weiss. 2010. Synthetic human elastin microfibers: Stable cross-linked tropoelastin and cell interactive constructs for tissue engineering applications. Acta Biomaterialia. 6:354-359.
Zhou, J., G. Hu, and X. Wang. 2010. Repression of Smooth Muscle Differentiation by a Novel High Mobility Group Box-containing Protein, HMG2L1. Journal of Biological Chemistry. 285:23177-23185.
2008
Zhang, M.-X., C. Zhang, Y.H. Shen, J. Wang, X.N. Li, Y. Zhang, J. Coselli, and X.L. Wang. 2008. Biogenesis of Short Intronic Repeat 27-Nucleotide Small RNA from Endothelial Nitric-oxide Synthase Gene. Journal of Biological Chemistry. 283:14685-14693.
Zhou, J., E. Blue, G. Hu, and P. Herring. 2008. Thymine DNA Glycosylase Represses Myocardin-induced Smooth Muscle Cell Differentiation by Competing with Serum Response Factor for Myocardin Binding. J Biol Chem, 283:35383-35392.
2007
Wu, K., C. Huang, J. Wei, L. Tsai, C. Hsu, Y. Chen, J. Yang, and C. Lin.  2007. Vasodilator action of docosahexaenoic acid (DHA) in human coronary arteries in vitro. The Chinese journal of physiology. 50:164-170.
2006
Jin, Y., E. Blue, and P. Gallagher. 2006. Control of Death-associated Protein Kinase (DAPK) Activity by Phosphorylation and Proteasomal Degradation. J Biol Chem, 281:39033-39040.
2005
Owing, J. 2005. Smoking and health: new research. Nova Publishers. HCASMC
2003
Wu, S.-N., P.-H. Lin, K.-S. Hsieh, Y.-C. Liu, and H.-T. Chiang. 2003. Behavior of Nonselective Cation Channels and Large-Conductance Ca2+-Activated K+ Channels Induced by Dynamic Changes in Membrane Stretch in Cultured Smooth Muscle Cells of Human Coronary Artery. Journal of Cardiovascular Electrophysiology. 14:44-51.
Wu, S.-N., H.-T. Chiang, F.-R. Chang, C.-C. Liaw, and Y.-C. Wu. 2003. Stimulatory Effects of Squamocin, an Annonaceous Acetogenin, on Ca2+-Activated K+ Current in Cultured Smooth Muscle Cells of Human Coronary Artery. Chemical Research in Toxicology. 16:15-22.
2002
Khachigian, L.M., R.G. Fahmy, G. Zhang, Y.V. Bobryshev, and A. Kaniaros. 2002. c-Jun Regulates Vascular Smooth Muscle Cell Growth and Neointima Formation after Arterial Injury. Journal of Biological Chemistry. 277:22985-22991.
Khachigian, L.M., R.G. Fahmy, G. Zhang, Y.V. Bobryshev, and A. Kaniaros. 2002. c-Jun Regulates Vascular Smooth Muscle Cell Growth and Neointima Formation after Arterial Injury: INHIBITION BY A NOVEL DNA ENZYME TARGETING c-Jun. Journal of Biological Chemistry. 277:22985-22991.