MSDS Cryopreserved Cells
Instructions HDLMVEC
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Description
Human Dermal Lymphatic Microvascular Endothelial (HDLMVEC) are isolated from lymphatic capillaries of normal human dermis. The lymphatic system interacts closely with the blood vascular system in maintaining tissue homeostasis. It plays a vital role in the body by regulating the immune system, transporting interstitial fluid, proteins and fat to the blood circulatory system. Lymphangiogenesis is also implicated in the metastatic process. Although lymphatic microvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features. Lymphatic capillaries have thin and discontinuous base membranes, and lymphatic endothelial cells, unlike their blood vessel counterparts, are not as tightly connected with each other, rendering the capillaries highly permeable.
HDLMVEC, together with Human Umbilical Vein Endothelial Cells, both from Cell Applications, Inc., have recently been used in a study demonstrating the key role of Prox1 and FOX C2 proteins in angiogenesis and lymphangiogenesis associated with oral squamous cell carcinoma progression.
Characterization: They express VEGFR3, and respond to activation by VEGF-C (see Figure on left).
(Cllick to Enlarge) Human Dermal Lymphatic Microvascular Cells (HDLMVEC) in culture (L). HDLMVEC immunolabeled for CD31/PECAM (green); nuclei are visualized with PI (red) (R).
Details
Tissue | Normal healthy human lymphatic capillaries from dermis |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Factor VIII-related Ag, CD31 (PECAM-1), DiI-Ac-LDL uptake |
Bioassay | Attach, spread, proliferate in Growth Med (AFS-coated surface) |
Cryovial | 500,000 HDLMVEC (3rd psg) frozen in Basal Medium w/ 10% FB, 10% DMSO |
Kit | Cryovial of HDLMVEC (100L-05a), Gr Med (111-500), Attchmnt Fctr Soln (123-100), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, 4th psg (flasks or plates) |
Doublings | At least 12 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Dermal Lymphatic Microvasular Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 100LK-05a | Price: $908.00 | |
| Cryopreserved Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Frozen HDLMVEC (5x10^5) | Size: 1 Cryovial | CAT.#: 100L-05a | Price: $650.00 | |
| Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 101L-25a | Price: $650.00 | |
| Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 101L-75a | Price: $840.00 | |
| Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 101L-24Wa | Price: $840.00 | |
| Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 101L-96Wa | Price: $960.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Microvascular Endothelial Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 100-500 | Price: $74.00 | |
| Microvascular Endothelial Cell Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 111-500 | Price: $148.00 | |
| Microvascular Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 111K-500 | Price: $158.00 | |
| Microvascular Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 111-GS | Price: $105.00 | |
| Microvascular Endothelial Cell Growth Medium Kit, components separate: Basal medium & growth supplement sold, together packaged separately. Supplements in individual vials. | Size: Yields 500 ml | CAT.#: 111KS-500 | Price: $160.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal CD31 Antibody: Monoclonal CD31 Antibody | Size: 100 ul | CAT.#: CB13678 | Price: $302.00 | |
| Anti-CD31: Mouse CD31 Antibody | Size: 100 ul | CAT.#: CC10038 | Price: $325.00 | |
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin | Size: 100 ml | CAT.#: 123-100 | Price: $22.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Dermal Lymphatic Microvasular Endothelial Cell RNA (HDLMVEC RNA), Adult: Total RNA prepared from Human Dermal Lymphatic Microvascular Endothelial Cells, adult | Size: 10 ug | CAT.#: 100L-R10a | Price: $465.00 | |
| Dermal Lymphatic Microvasular Endothelial Cell RNA (HDLMVEC RNA), Adult: Total RNA prepared from Human Dermal Lymphatic Microvascular Endothelial Cells, adult | Size: 25 ug | CAT.#: 100L-R25a | Price: $930.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Human PECAM-1/CD31 ELISA Kit: Human Platelet Endothelial Cell Adhesion Molecule-1/Cluster of Differentiation 31 ELISA Kit | Size: 96 Wells | CAT.#: CL0883 | Price: $587.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2014
Sasahira, T. N. Ueda, K. Yamamoto, M. Kurihara, S. Matsushima, U. Bhawal, T. Kirita and H. Kuniyasu. 2014. Prox1 and FOXC2 Act as Regulators of Lymphangiogenesis and Angiogenesis in Oral Squamous Cell Carcinoma. PLoS ONE, dx.doi.org/10.1371/journal.pone.0092534.
Sasahira, T., T. Kirita, K. Yamamoto, N. Ueda, M. Kurihara, S. Matsushima, U. Bhawal, A. Bosserhoff and H. Kuniyasu. 2014. Transport and Golgi organisation protein 1 is a novel tumour progressive factor in oral squamous cell carcinoma. Eur J Cancer, 50-2142-2151.
2004
Milner, P., P. Bodin, S. Guiducci, A. Del Rosso, M. Kahaleh, M. Matucci-Cerinic, and G. Burnstock. 2004. Regulation of substance P mRNA expression in human dermal microvascular endothelial cells. Clinical and experimental rheumatology. 22:S24-S27.
2003
Duan, D., and S. Rosen. 2003. Bone marrow specific protein. Patent Application US 20030191062 A1.
2001
Suh, W.-H. 2001. Cell-specific targeting polymeric gene delivery carriers. The University of Utah, PhD dissertation.