MSDS Cryopreserved Cells
Instructions HDLMVEC
5 Important Cell Culture Rules
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Description

Human Dermal Lymphatic Microvascular Endothelial (HDLMVEC) are isolated from lymphatic capillaries of normal human dermis. The lymphatic system interacts closely with the blood vascular system in maintaining tissue homeostasis.  It plays a vital role in the body by regulating the immune system, transporting interstitial fluid, proteins and fat to the blood circulatory system.  Lymphangiogenesis is also implicated in the metastatic process.  Although lymphatic microvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features.  Lymphatic capillaries have thin and discontinuous base membranes, and lymphatic endothelial cells, unlike their blood vessel counterparts, are not as tightly connected with each other, rendering the capillaries highly permeable.
HDLMVEC, together with Human Umbilical Vein Endothelial Cells, both from Cell Applications, Inc., have recently been used in a study demonstrating the key role of Prox1 and FOX C2 proteins in angiogenesis and lymphangiogenesis associated with oral squamous cell carcinoma progression.
Characterization: They express VEGFR3, and respond to activation by VEGF-C (see Figure on left).
(Cllick to Enlarge)  Human Dermal Lymphatic Microvascular Cells (HDLMVEC) in culture (L). HDLMVEC immunolabeled for CD31/PECAM (green); nuclei are visualized with PI (red) (R).

Details

Tissue
Normal healthy human lymphatic capillaries from dermis
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag, CD31 (PECAM-1), DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med (AFS-coated surface)
Cryovial
500,000 HDLMVEC (3rd psg) frozen in Basal Medium w/ 10% FB, 10% DMSO
Kit
Cryovial of HDLMVEC (100L-05a), Gr Med (111-500), Attchmnt Fctr Soln (123-100), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Tsfr Med, 4th psg (flasks or plates)
Doublings
At least 12
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HDLMVEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved Human Dermal Lymphatic Microvasular Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 100LK-05a Price: $908.00
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Cryopreserved Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Frozen HDLMVEC (5x10^5) Size: 1 Cryovial CAT.#: 100L-05a Price: $650.00
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Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 101L-25a Price: $650.00
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Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 101L-75a Price: $840.00
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Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 101L-24Wa Price: $840.00
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Proliferating Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC), adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 101L-96Wa Price: $960.00
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Related Products

Product Size CAT.# Price Quantity
Microvascular Endothelial Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 100-500 Price: $74.00
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Microvascular Endothelial Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 111-500 Price: $148.00
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Microvascular Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 111K-500 Price: $158.00
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Microvascular Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 111-GS Price: $105.00
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Microvascular Endothelial Cell Growth Medium Kit, components separate: Basal medium & growth supplement sold, together packaged separately. Supplements in individual vials. Size: Yields 500 ml CAT.#: 111KS-500 Price: $160.00
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Extended Family Products

Product Size CAT.# Price Quantity
Monoclonal CD31 Antibody: Monoclonal CD31 Antibody Size: 100 ul CAT.#: CB13678 Price: $302.00
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Anti-CD31: Mouse CD31 Antibody Size: 100 ul CAT.#: CC10038 Price: $325.00
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.00
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Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.00
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Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin Size: 100 ml CAT.#: 123-100 Price: $22.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00
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Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $62.00
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Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $496.00
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Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $587.00
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Dermal Lymphatic Microvasular Endothelial Cell RNA (HDLMVEC RNA), Adult: Total RNA prepared from Human Dermal Lymphatic Microvascular Endothelial Cells, adult Size: 10 ug CAT.#: 100L-R10a Price: $465.00
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Dermal Lymphatic Microvasular Endothelial Cell RNA (HDLMVEC RNA), Adult: Total RNA prepared from Human Dermal Lymphatic Microvascular Endothelial Cells, adult Size: 25 ug CAT.#: 100L-R25a Price: $930.00
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Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $517.00
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Human PECAM-1/CD31 ELISA Kit: Human Platelet Endothelial Cell Adhesion Molecule-1/Cluster of Differentiation 31 ELISA Kit Size: 96 Wells CAT.#: CL0883 Price: $587.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $63.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $194.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,090.00
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Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $533.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,499.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

Downoad Now
Cell Apps Flyer Skin Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2014
Sasahira, T. N. Ueda, K. Yamamoto, M. Kurihara, S. Matsushima, U. Bhawal, T. Kirita and H. Kuniyasu. 2014. Prox1 and FOXC2 Act as Regulators of Lymphangiogenesis and Angiogenesis in Oral Squamous Cell CarcinomaPLoS ONE, dx.doi.org/10.1371/journal.pone.0092534.
Sasahira, T., T. Kirita, K. Yamamoto, N. Ueda, M. Kurihara, S. Matsushima, U. Bhawal, A. Bosserhoff and H. Kuniyasu. 2014. Transport and Golgi organisation protein 1 is a novel tumour progressive factor in oral squamous cell carcinomaEur J Cancer, 50-2142-2151.
2004
Milner, P., P. Bodin, S. Guiducci, A. Del Rosso, M. Kahaleh, M. Matucci-Cerinic, and G. Burnstock. 2004. Regulation of substance P mRNA expression in human dermal microvascular endothelial cells. Clinical and experimental rheumatology. 22:S24-S27.
2003
Duan, D., and S. Rosen. 2003. Bone marrow specific protein. Patent Application US 20030191062 A1.
2001
Suh, W.-H. 2001. Cell-specific targeting polymeric gene delivery carriers. The University of Utah, PhD dissertation.