Instructions Gastrointestinal EpC
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

The intestinal epithelium is a single layer of cells organized into crypts and villi, known as the most rapidly self-renewing tissue in adult mammals. The cells that line the intestinal lumen perform the primary functions of digestion, water and nutrient absorption, and forms a barrier against luminal pathogens.

Transit-amplifying cells spend approximately two days in the intestinal crypts, dividing 4–5 times before terminally differentiating into specialized intestinal epithelial cell types. In the small intestine, the surface area is dramatically enlarged through epithelial protrusions called villi. Three days after their terminal differentiation, the cells reach the tip of the villus, undergo spontaneous apoptosis, and are shed into the gut lumen.

CAI’s intestinal epithelial culture system provides outstanding resource for investigation of intestinal epithelial cell physiology related to GI infection, inflammatory bowel disease (IBD) like Crohn’s disease, ulcerative colitis, and intestinal cancer. Our epithelial cell culture system can be efficiently used as a test platform for the potential drug candidates and disease modulators. Other applications of this culture system include functional analysis of intestinal epithelium, GI disease modeling, and regenerative therapy preclinical testing such as drug compound screening and other validation assays.

With optimized, defined culture media from CAI, the Intestinal Epithelial Cells can be seeded and maintained for as long as 8 days. Epithelial Cells grown in CAI medium form a monolayer of polarized epithelial cells with tight junction formation as evidenced by Villin (apical marker), Na+/K+ ATPase (basolateral marker), ZO-1 (tight junction marker) and pan-Cytokeratin (epithelial marker) staining.

Human Duodenum Epithelial Cells (HDuEpC) and other Adult GI EpC monolayers. Top left: Phase contrast of Ileum EpC. Top right (Red): ZO-1 of Duedenum EpC. Bottom left (Green): Na+/K+ ATPase of Colon EpC. Bottom middle (Red): pan-Cytokeratin of Gastric EpC. Bottom right (Green)  Villin of Jejunum EpC. Blue: DAPI.

Details

TissueNormal healthy human duodenum.
QCNo bacteria, yeast, fungi, mycoplasma, virus.
BioassayAttach, spread, in Culture Medium.
Cryovial500,000 or 1,000,000 HDuEpC in Freezing Medium.
KitCryovial frozen HDuEpC (732Du-05a, 732Du-10a), Culture Med (716DCM-50), Coating Solution (025-05), Thawing Solution (716T-20).
CulturedShipped in flasks or plates in medium.
DoublingsCells do not divide and cannot be passaged.
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

Instructions Gastrointestinal EpC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HDuEpC Total Kit, adult: 5x10^5 Cells (adult), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732DuK-05a Price: $685.00
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Cryopreserved HDuEpC Total Kit, adult: 1x10^6 Cells (adult), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732DuK-10a Price: $852.00
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Cryopreserved HDuEpC, adult: Frozen HDuEpC (5x10^5) Size: 1 Cryovial CAT.#: 732Du-05a Price: $595.00
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Cryopreserved HDuEpC, adult: Frozen HDuEpC (1x10^6) Size: 1 Cryovial CAT.#: 732Du-10a Price: $762.00
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Cultured HDuEpC, adult: Pre-plated, cultured cells in medium Size: - CAT.#: 733Du-XXa Price: $0.00
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Related Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Coating Solution: Coating solution for GI Epithelial Cells Size: 5 ml CAT.#: 025-05 Price: $16.00
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GI Epithelial Cell Defined Culture Medium: All-in-one ready-to-use. Use within 30 days of receipt. Size: 50 ml CAT.#: 716DCM-50 Price: $55.00
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GI Epithelial Cell Defined Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 50 ml CAT.#: 716DCMK-50 Price: $75.00
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GI Epithelial Cell Thawing Solution: Thawing solution for GI Epithelial Cells Size: 20 ml CAT.#: 716T-20 Price: $26.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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