MSDS 211-500
MSDS 211K-500
MSDS 210-500
MSDS 211-GS
Description
Media were optimized for the robust growth and the maintenance of phenotypic and functional characteristics of Endothelial Cells in culture. Media were designed with attention to detail that ensures the consistency of cell health, viability, performance, physiology, morphology and function.
Use with:
- Human Aortic Endothelial Cells: HAOEC
- Human Femoral Artery Endothelial Cells: HFAEC
- Human Pulmonary Artery Endothelial Cells: HPAEC
- Human Umbilical Vein Endothelial Cells: HUVEC
EGM v2 (Cat# 213-500) supports continuous, robust growth
HUVEC were seeded to T25 flasks, passaged repeatedly, and counted for cell number.
Sustained expression of endothelial cell markers
Growth of primary Human Aortic Endothelialial Cells (HAOEC) in EGM (Cat# 211-500) maintains CD31 expression.
Following treatment of TNF-alpha, Human Coronary Artery Endothelial cells (HCAEC) upregulate VCAM-1 (right). Untreated (left)
For 10 days, cells were stimulated with VEGF or VEGF in the presence of the angiogenesis inhibitor suramin.
Transfect primary endothelial cells with Cytofect Transfection Kits
Convenient, non-toxic transfection of HAOEC with GFP plasmid.
According to Sara Lynn Farwell, Ph.D. Candidate, Cell & Molecular Bio. at Lehigh University: “CAI’s Endothelial Cell Growth Medium is reliable for our experiments, as well as general culturing. There must be magic in their media, because when we had problems with other formulations, the CAI product fixed the issues!”
Note that endothelial Cell Defined Medium is designed for maintaining the monolayer of endothelial cells at 100% confluent culture. It does not contain the growth factors VEGF, FGF or PDGF, nor does it contain serum, but is instead supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors.
Choose the Human Endothelial Cell Medium best suited for your specific applications. Optimized for in vitro culture of a variety of Endothelial Cells from aorta, artery and vein. EGM (Cat# 211-500) is optimized for angiogenesis and other more physiological studies (does not contain VEGF). EGM v2 (Cat# 213-500) is enriched with growth factors for accelerated growth, and includes VEGF, FBS, EGF, bFGF, IGF, Ascorbic acid, Hydrocortisone, Heparin.
Details
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions. The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211-500 | Price: $121.00 | |
| Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: Yields 500 ml | CAT.#: 211K-500 | Price: $143.00 | |
| Human EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 210-500 | Price: $91.00 | |
| Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 15 ml | CAT.#: 211-GS | Price: $74.00 | |
| Human EC Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. | Size: 500 ml | CAT.#: 213-500 | Price: $141.00 | |
| Endothelial Cells Growth Medium V2 Kit, 500ml: Basal medium & growth supplement sold together packaged separately. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211K-500. | Size: 500 ml | CAT.#: 213K-500 | Price: $144.00 | |
| Human EC Growth Medium Kit V2, components separate: Basal medium & growth supplement sold together packaged separately. Supplements in individual vials. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211K-500. | Size: 500 ml | CAT.#: 213KS-500 | Price: $155.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in confluent cultures. Not for cell growth. | Size: 500 ml | CAT.#: 113-500 | Price: $130.00 | |
| Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. | Size: 500 ml | CAT.#: 113PR-500 | Price: $136.00 | |
| Human EC Growth Medium wo Antibiotics: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. Suitable for Cytofect Transfection Kit. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211A-500 | Price: $130.00 | |
| Human EC Growth Medium wo FBS: 211-500 growth medium, but without FBS. FBS must be added in order to support cell culture. | Size: 500 ml | CAT.#: 211F-500 | Price: $130.00 | |
| Human EC Growth Medium wo Hydrocortisone: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211H-500 | Price: $130.00 | |
| Human EC Growth Medium wo Phenol Red: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211PR-500 | Price: $130.00 | |
| Human EC Basal Medium wo Phenol Red: Basal medium without growth supplement and phenol red | Size: 500 ml | CAT.#: 210PR-500 | Price: $98.00 | |
| Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics | Size: 15 ml | CAT.#: 211A-GS | Price: $88.00 | |
| Human EC Growth Supplement wo FBS: Growth supplement without FBS. FBS must be added in order to support cell culture. | Size: 5 ml | CAT.#: 211F-GS | Price: $91.00 | |
| Human EC Growth Supplement wo Hydrocortisone: Growth supplement without hydrocortisone | Size: 15 ml | CAT.#: 211H-GS | Price: $88.00 | |
| Human EC Growth Medium V2 without FBS: Endothlelial Cell Growth Medium v2, without FBS. | Size: 500 ml | CAT.#: 213F-500 | Price: $153.00 | |
| Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | Size: 250 ml | CAT.#: 209-250 | Price: $81.00 | |
| Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red | Size: 250 ml | CAT.#: 209PR-250 | Price: $91.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| HUVEC Conditioned Medium: HUVEC Conditioned Medium | Size: 5 ml | CAT.#: 211CM-05 | Price: $55.00 |
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2017
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2016
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2015
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2014
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2012
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2011
Konakahara, S., M. Saitou, S. Hori, T. Nakane, K. Murai, R. Itoh, A. Shinsaka, J. Kohroki, T. Kawakami, M. Kajikawa, and Y. Masuho. 2011. A neuronal transmembrane protein LRFN4 induces monocyte/macrophage migration via actin cytoskeleton reorganization. FEBS letters. 585:2377-2384.
Mikkelsen, L., M. Sheykhzade, K.A. Jensen, A.T. Saber, N.R. Jacobsen, U. Vogel, H. Wallin, S. Loft, and P. Moller. 2011. Modest effect on plaque progression and vasodilatory function in atherosclerosis-prone mice exposed to nanosized TiO2. Part Fibre Toxicol. 8:32.
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Sharma, V., and H.H. Freeze. 2011a. Mannose Efflux from the Cells: A POTENTIAL SOURCE OF MANNOSE IN BLOOD. Journal of Biological Chemistry. 286:10193-10200.
Sharma, V., M. Ichikawa, P. He, Y. Bravo, R. Dahl, B. Ng, N. Cosford, and H. Freeze. 2011. Phosphomannose Isomerase Inhibitors Improve N-Glycosylation in Selected Phosphomannomutase-deficient Fibroblasts. JCB, 286:39431-39438.
Shatanawi, A., M.J. Romero, J.A. Iddings, S. Chandra, N.S. Umapathy, A.D. Verin, R.B. Caldwell, and R.W. Caldwell. 2011. Angiotensin II-induced vascular endothelial dysfunction through RhoA/Rho kinase/p38 mitogen-activated protein kinase/arginase pathway. American Journal of Physiology - Cell Physiology. 300:C1181-C1192.
Wang, H.-J., T.-L. Lu, H. Huang, and H.-C. Huang. 2011. Paclitaxel induces thrombomodulin downregulation in human aortic endothelial cells. Texas Heart Institute Journal. 38:20.
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Yang, C.J., C.Y. Lin, T.-c. Hsieh, S.C. Olson, and J.M. Wu. 2011. Control of eotaxin-1 expression and release by resveratrol and its metabolites in culture human pulmonary artery endothelial cells. American journal of cardiovascular disease. 1:16.
Yew, T.-L., Y.-T. Hung, H.-Y. Li, H.-W. Chen, L.-L. Chen, K.-S. Tsai, S.-H. Chiou, K.-C. Chao, T.-F. Huang, H.-L. Chen, and S.-C. Hung. 2011. Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation. Cell Transplantation. 20:693-706.
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2010
Hung, C.-H., D. Wu, F.-Y. Lin, R.-Y. Yuan, and C.-J. Hu. 2010. Toll-like Receptor 4 and Vascular Cell Adhesion Molecule 1 in Monocyte-Endothelium Adhesion Induced by Lipopolysaccharide. J. Experimental & Clinical Medicine. 2:297-301.
Blasberg, J.D., C.M. Goparaju, H.I. Pass, and J.S. Donington. 2010. Lung cancer osteopontin isoforms exhibit angiogenic functional heterogeneity. The Journal of Thoracic and Cardiovascular Surgery. 139:1587-1593.
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Gelissen, I.C., S. Cartland, A.J. Brown, C. Sandoval, M. Kim, D.L. Dinnes, Y. Lee, V. Hsieh, K. Gaus, L. Kritharides, and W. Jessup. 2010. Expression and stability of two isoforms of ABCG1 in human vascular cells. Atherosclerosis. 208:75-82.
Hou, X., J. Song, X.-N. Li, L. Zhang, X. Wang, L. Chen, and Y.H. Shen. 2010. Metformin reduces intracellular reactive oxygen species levels by upregulating expression of the antioxidant thioredoxin via the AMPK-FOXO3 pathway. Biochemical and biophysical research communications. 396:199-205.
Li, R., Z. Ning, J. Cui, F. Yu, C. Sioutas, and T. Hsiai. 2010. Diesel exhaust particles modulate vascular endothelial cell permeability: Implication of ZO-1 expression. Toxicology letters. 197:163-168.
Miyake, Y., M. Mochizuki, C. Ito, M. Itoigawa, and T. Osawa. 2010. Peroxynitrite Scavengers Produced by Filamentous Fungus Used in the Katsuobushi Manufacturing Process. Food science and technology research. 16:493-498.
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Patel, N.S., V.S. Mathura, C. Bachmeier, D. Beaulieu-Abdelahad, V. Laporte, O. Weeks, M. Mullan, and D. Paris. 2010. Alzheimer’s β-amyloid peptide blocks vascular endothelial growth factor mediated signaling via direct interaction with VEGFR-2. Journal of Neurochemistry. 112:66-76.
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2009
Duffy, G., E. Byrne, T. McFadden, E. Farrell, and F. O’Brien. 2009. IN VITRO VASCULARISATION OF COLLAGEN-GAG SCAFFOLDS USING MESENCHYMAL STEM CELLS. Eu Cells and Materials 18:16.
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Himmelhaus, M., and A. Francois. 2009. In-vitro sensing of biomechanical forces in live cells by a whispering gallery mode biosensor. Biosensors and Bioelectronics. 25:418-427.
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Huang, C., G. Li, H. Dong, S. Sun, H. Chen, D. Luo, L. Sun, X. Li, Z. Chen, H. Yang, S. Wei and Y. Zhou. 2009. Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155. Int J Molec Med, http://dx.doi.org/10.3892/ijmm.2015.2192.
Li, R., T. Beebe, J. Cui, M. Rouhanizadeh, L. Ai, P. Wang, M. Gundersen, W. Takabe, and T.K. Hsiai. 2009. Pulsatile shear stress increased mitochondrial membrane potential: Implication of Mn-SOD. Biochemical and biophysical research communications. 388:406-412.
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