Instructions CADMEC
Instructions HLMVEC
Instructions HAOEC
Instructions HPAEC
Instructions HUVEC
MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Flyer HUVEC
Cell Appls Flyer Skin Cells
Cell Apps Poster Primary Cells

Description

Human Endothelial Cells: Pre-Screened for Angiogenesis & VEGF Signaling - The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC).  At Cell Applications, select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.  Other cell types can be pre-screened by customer request.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells -VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells.  VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells.  VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
(Click to Enlarge) Pre-Screened Endothelial Cells  Top: Activation of VEGFR-2 signaling in HAOEC, HUVEC and HMVEC by 100 ng/ml VEGF for 0, 5 or 15 minutes.  VEGFR-2 autophosphorylation probed w/ phospho-VEGFR-2 (Y1175) specific Ab, Akt activation detected by phospho-Akt (S473) specific antibody, and MAP kinase activation detected using phospho-p44/42 MAP kinase (T202/Y204) Ab.  Bottom: Pre-screened HUVEC & HMVEC express key endothelial markers Tie2, eNOS, Axl and Etk/Bmx. 

Details

Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells. Prescreened Endothelial Cells from Cell Applications, Inc. express the following important biomarkers:
  • Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes
  • eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function
  • Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product), is implicated in cell survival, leukocyte transmigration and neointima formation
  • Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation and survival.
Instructions CADMEC

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Instructions HLMVEC

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Instructions HLMVEC

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Instructions HAOEC

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Instructions HAOEC

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Instructions HAOEC

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Instructions HPAEC

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Instructions HPAEC

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Instructions HUVEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved CADMEC, Pre-Screened, Adult: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S100K-05a Price: $933.00
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Cryopreserved CADMEC, Pre-Screened, Adult: Frozen CADMEC/HMVEC (5x10^5) Size: 1 Cryovial CAT.#: S100-05a Price: $726.00
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Cryopreserved CADMEC, Pre-Screened, Neonatal: 5x10^5 Cells (Pre-Screened, Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S100K-05n Price: $912.00
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Cryopreserved CADMEC, Pre-Screened, Neonatal: Frozen CADMEC (5x10^5) Size: 1 Cryovial CAT.#: S100-05n Price: $705.00
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Cryopreserved HAOEC, Pre-Screened, Adult: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S304K-05a Price: $957.00
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Cryopreserved HAOEC, Pre-Screened, Adult: Frozen HAOEC (5x10^5) Size: 1 Cryovial CAT.#: S304-05a Price: $807.00
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Cryopreserved HLMVEC, Pre-Screened, Adult: Frozen HLMVEC (5x10^5) Size: 1 Cryovial CAT.#: S540-05a Price: $869.00
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Cryopreserved HLMVEC, Pre-Screened, Adult: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S540K-05a Price: $1,092.00
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Cryopreserved HPAEC-AS, Pre-Scr, Adult: 5x10^5 Cells (from donor with Asthma, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S302ASK-05a Price: $1,001.00
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Cryopreserved HPAEC-AS, Pre-Scr, Adult: Frozen HPAEC-AS from donor with Asthma (5x10^5) Size: 1 Cryovial CAT.#: S302AS-05a Price: $851.00
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Cryopreserved HUVEC, Pre-Screened, Neonatal : 5x10^5 Cells (Pre-Screened, Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S200K-05n Price: $575.00
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Cryopreserved HUVEC, Pre-Screened, Neonatal : Frozen HUVEC (5x10^5) Size: 1 Cryovial CAT.#: S200-05n Price: $425.00
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Proliferating CADMEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: S101-25a Price: $726.00
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Proliferating CADMEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: S101-75a Price: $931.00
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Proliferating CADMEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: S101-6Wa Price: $931.00
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Proliferating CADMEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: S101-96Wa Price: $1,060.00
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Proliferating CADMEC, Pre-Screened, Neonatal : Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: S101-25n Price: $705.00
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Proliferating CADMEC, Pre-Screened, Neonatal : Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: S101-75n Price: $909.00
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Proliferating CADMEC, Pre-Screened, Neonatal : Actively growing, dividing cells in medium Size: 6 Well CAT.#: S101-6Wn Price: $909.00
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Proliferating CADMEC, Pre-Screened, Neonatal : Actively growing, dividing cells in medium Size: 96 Well CAT.#: S101-96Wn Price: $1,039.00
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Proliferating HAOEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: S305-25a Price: $807.00
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Proliferating HAOEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: S305-75a Price: $1,012.00
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Proliferating HAOEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: S305-6Wa Price: $1,012.00
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Proliferating HAOEC, Pre-Screened, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: S305-96Wa Price: $1,141.00
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Proliferating HUVEC, Pre-Screened, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: S201-25n Price: $425.00
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Proliferating HUVEC, Pre-Screened, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: S201-75n Price: $630.00
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Proliferating HUVEC, Pre-Screened, Neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: S201-6Wn Price: $630.00
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Proliferating HUVEC, Pre-Screened, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: S201-96Wn Price: $759.00
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Related Products

Product Size CAT.# Price Quantity
Human EC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 210-500 Price: $70.00
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Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211-500 Price: $105.00
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Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: Yields 500 ml CAT.#: 211K-500 Price: $124.00
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Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 15 ml CAT.#: 211-GS Price: $57.00
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Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics Size: 15 ml CAT.#: 211A-GS Price: $68.00
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Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in 100% confluent culture. Not for cell growth. Size: 500 ml CAT.#: 113-500 Price: $113.00
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Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. Size: 500 ml CAT.#: 113PR-500 Price: $118.00
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Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 209-250 Price: $70.00
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Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red Size: 250 ml CAT.#: 209PR-250 Price: $79.00
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Human Meso Endo Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 212-500 Price: $118.00
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Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 212K-500 Price: $118.00
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Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red Size: yields 500 ml CAT.#: 212KPR-500 Price: $130.00
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Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 212A-500 Price: $124.00
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Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS Size: 500 ml CAT.#: 212F-500 Price: $136.00
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Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red Size: 500 ml CAT.#: 212PR-500 Price: $118.00
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Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 212-GS Price: $62.00
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Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS Size: 5 ml CAT.#: 212F-GS Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
CADMEC RNA: Total RNA prepared from Human Dermal Microvascular Endothelial Cells, adult Size: 10 ug CAT.#: 100-R10a Price: $350.00
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CADMEC RNA: Total RNA prepared from Human Dermal Microvascular Endothelial Cells, adult Size: 25 ug CAT.#: 100-R25a Price: $700.00
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CADMEC RNA: Total RNA prepared from Human Dermal Microvscular Endothelial Cells, neonatal Size: 10 ug CAT.#: 100-R10n Price: $350.00
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CADMEC RNA: Total RNA prepared from Human Dermal Microvscular Endothelial Cells, neonatal Size: 25 ug CAT.#: 100-R25n Price: $700.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HAOEC RNA, Adult: Total RNA prepared from Human Aortic Endothelial Cells, adult Size: 10 ug CAT.#: 304-R10a Price: $350.00
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HAOEC RNA, Adult: Total RNA prepared from Human Aortic Endothelial Cells, adult Size: 25 ug CAT.#: 304-R25a Price: $700.00
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HPAEC RNA, Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult Size: 10ug CAT.#: 302-R10a Price: $350.00
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HPAEC RNA, Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult Size: 25ug CAT.#: 302-R25a Price: $700.00
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Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $587.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.00
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Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $517.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,090.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $194.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $533.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,499.00
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Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.00
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HUVEC RNA, Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, neonatal Size: 10 ug CAT.#: 200-R10n Price: $350.00
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HUVEC RNA, Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, neonatal Size: 25 ug CAT.#: 200-R25n Price: $700.00
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Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.00
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Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Airway Cells

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Cell Apps Flyer Cardiovascular Cells

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Cell Apps Flyer Endothelial Cells

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Cell Apps Flyer HUVEC

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Cell Apps Poster Primary Cells

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