Instructions HFAEC
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Femoral Artery Endothelial Cells, isolated from normal tissue, are cryopreserved at 2nd passage and can be cultured and propagated to 5th passage and beyond without losing their morphologic and phenotypic characteristics. The femoral artery, a large artery in the thigh, provides the main blood supply to the lower limb. It can be palpated through the skin to obtain the femoral pulse, and is often used for catheter access. As the femoral artery is susceptible to peripheral arterial disease, HCAEC are commonly used to study atherosclerosis.

(Click to Enlarge) Human Femoral Artery Endothelial Cells: HFAEC (A), Phase (B) and Composite with DiI-Ac-LDL staining (C).

Details

Tissue
Normal healthy human femoral artery 
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag, DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HFAEC (2nd Passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HFAEC (3016-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HFAEC

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cryopreserved HFAEC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 3016K-05a Price: $961.70
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Cryopreserved HFAEC, adult: Frozen HFAEC (5x10^5) Size: 1 Cryovial CAT.#: 3016-05a Price: $807.70
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Related Products

Product Size CAT.# Price Quantity
Human Endothelial Cell Basal Medium: Basal medium without growth supplement and phenol red Size: 500 ml CAT.#: 210PR-500 Price: $75.34
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Human Endothelial Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 210-500 Price: $69.96
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Human Endothelial Cell Defined Medium: Defined medium without serum Size: 500 ml CAT.#: 113-500 Price: $113.01
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Human Endothelial Cell Defined Medium: Defined medium without serum and phenol red Size: 500 ml CAT.#: 113PR-500 Price: $118.39
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Human Endothelial Cell Growth Medium: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211PR-500 Price: $113.01
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Human Endothelial Cell Growth Medium: Growth medium without FBS. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211F-500 Price: $113.01
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Human Endothelial Cell Growth Medium: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211A-500 Price: $113.01
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Human Endothelial Cell Growth Medium: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211H-500 Price: $113.01
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Human Endothelial Cell Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211-500 Price: $105.47
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Human Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: Yields 500 ml CAT.#: 211K-500 Price: $123.77
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Human Endothelial Cell Growth Medium v2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. Size: 500 ml CAT.#: 213-500 Price: $123.00
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Human Endothelial Cell Growth Supplement: Growth supplement without FBS Size: 5 ml CAT.#: 211F-GS Price: $69.96
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Human Endothelial Cell Growth Supplement: Growth supplement without antibiotics Size: 15 ml CAT.#: 211A-GS Price: $67.80
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Human Endothelial Cell Growth Supplement: Growth supplement without hydrocortisone Size: 15 ml CAT.#: 211H-GS Price: $67.80
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Human Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 15 ml CAT.#: 211-GS Price: $57.04
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Human Endothelial Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 209-250 Price: $69.96
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Human Endothelial Cell Starvation Medium: Starvation medium without phenol red Size: 250 ml CAT.#: 209PR-250 Price: $78.57
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Anti-VEGF: Polyclonal Vascular Endothelial Growth Factor Antibody Size: 100 ul CAT.#: CA1080 Price: $302.38
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Anti-VEGF-C: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.38
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Anti-VEGFR1: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.13
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $53.76
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $430.50
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E-Selectin ELISA Kit, Human: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $586.56
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ICAM-1 ELISA Kit, Human: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.31
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $193.73
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.31
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,174.94
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P-Selectin ELISA Kit, Human: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $516.60
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $193.73
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VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.31
-+
VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,089.75
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VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.10
-+
VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $532.74
-+
VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,498.73
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VEGF-C ELISA Kit, Human: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.18
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Resources/Documents

Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now