Instructions HFLS-OA
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Description
Human Fibroblast-Like Synoviocytes: Osteoarthritis (HFLS-OA) provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of osteoarthritis.
HFLS-OA from Cell Applications, Inc. have been shown to:
- Possess osteogenic, chondrogenic and adipogenic potentials
- Express about half of the mesenchymal stem cell related transcription factors and molecular markers identified in a comprehensive study
- Involve LPA–EDG2 signaling in the pathogenesis of osteoarthritis via catabolic processes
- Employ parvorvirus B19 capsid proteins to activate synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy
Finally, all three types of HFLS (normal, RA and OA), all from Cell Applications, Inc. were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms.
(Click to Enlarge) Human Fibroblast-Like Synoviocytes-Osteoathritis: HFLS-OA, are isolated from the tissue of donors with arthritic disease.
Details
Tissue | Human synovial tissue from donor with osteoarthrits |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | 500,000 HFLS-OA (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Croyvial frozen HFLS-OA (408OA-05a), Gr Med (415-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 5 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Fibroblast-Like Synoviocyte-Osteo Arthritis Total Kit, adult: 5x10^5 Cells (from donor with Osteoarthritis, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 408OAK-05a | Price: $1,115.00 | |
| Cryopreserved Synoviocytes, Osteoarthritis (HFLS-OA), adult: Frozen HFLS from donor with Osteoarthritis (5x10^5) | Size: 1 Cryovial | CAT.#: 408OA-05a | Price: $945.00 | |
| Proliferating Synoviocytes, Osteoarthritis (HFLS-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: T-25 Flask | CAT.#: 409OA-25a | Price: $945.00 | |
| Proliferating Synoviocytes, Osteoarthritis (HFLS-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: T-75 Flask | CAT.#: 409OA-75a | Price: $1,135.00 | |
| Proliferating Synoviocytes, Osteoarthritis (HFLS-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: 96 Well | CAT.#: 409OA-96Wa | Price: $1,255.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HFLS Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 414-500 | Price: $70.00 | |
| HFLS Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 415-500 | Price: $117.00 | |
| HFLS Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 415K-500 | Price: $128.00 | |
| HFLS Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 415-GS | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Synoviocytes, Osteoarthritis RNA (HFLS-OA RNA), Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes-Osteoarthritis, adult | Size: 10 ug | CAT.#: 408OA-R10a | Price: $398.00 | |
| Synoviocytes, Osteoarthritis RNA (HFLS-OA RNA), Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes-Osteoarthritis, adult | Size: 25 ug | CAT.#: 408OA-R25a | Price: $796.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2009Kubo, H., M. Shimizu, Y. Taya, T. Kawamoto, M. Michida, E. Kaneko, A. Igarashi, M. Nishimura, K. Segoshi, Y. Shimazu, K. Tsuji, T. Aoba, and Y. Kato. 2009. Identification of mesenchymal stem cell (MSC)-transcription factors by microarray and knockdown analyses, and signature molecule-marked MSC in bone marrow by immunohistochemistry. Genes to Cells. 14:407-424.2008Mototani, H., A. Iida, M. Nakajima, T. Furuichi, Y. Miyamoto, T. Tsunoda, A. Sudo, A. Kotani, A. Uchida, K. Ozaki, Y. Tanaka, Y. Nakamura, T. Tanaka, K. Notoya, and S. Ikegawa. 2008. A functional SNP in EDG2 increases susceptibility to knee osteoarthritis in Japanese. Human molecular genetics. 17:1790-1797.2006Lu, J., N. Zhi, S. Wong, and K.E. Brown. 2006. Activation of synoviocytes by the secreted phospholipase A2 motif in the VP1-unique region of parvovirus B19 minor capsid protein. Journal of Infectious Diseases. 193:582-590.