Instructions HFLS-OA
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Fibroblast-Like Synoviocytes: Osteoarthritis (HFLS-OA) provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of osteoarthritis.
HFLS-OA from Cell Applications, Inc. have been shown to:
  • Possess osteogenic, chondrogenic and adipogenic potentials
  • Express about half of the mesenchymal stem cell related transcription factors and molecular markers identified in a comprehensive study
  • Involve LPA–EDG2 signaling in the pathogenesis of osteoarthritis via catabolic processes
  • Employ parvorvirus B19 capsid proteins to activate synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy
Finally, all three types of HFLS (normal, RA and OA), all from Cell Applications, Inc. were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms.
(Click to Enlarge) Human Fibroblast-Like Synoviocytes-Osteoathritis: HFLS-OA, are isolated from the tissue of donors with arthritic disease.

Details

Tissue
Human synovial tissue from donor with osteoarthrits
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
500,000 HFLS-OA (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Croyvial frozen HFLS-OA (408OA-05a), Gr Med (415-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 5
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HFLS-OA

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HFLS-OA, Adult: 5x10^5 Cells (from donor with Osteoarthritis, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 408OAK-05a Price: $891.00
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Cryopreserved HFLS-OA, Adult: Frozen HFLS from donor with Osteoarthritis (5x10^5) Size: 1 Cryovial CAT.#: 408OA-05a Price: $748.00
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Proliferating HFLS-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: T-25 Flask CAT.#: 409OA-25a Price: $748.00
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Proliferating HFLS-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: T-75 Flask CAT.#: 409OA-75a Price: $952.00
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Proliferating HFLS-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: 6 Well CAT.#: 409OA-6Wa Price: $914.00
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Proliferating HFLS-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: 96 Well CAT.#: 409OA-96Wa Price: $1,043.00
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Related Products

Product Size CAT.# Price Quantity
HFLS Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 414-500 Price: $54.00
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HFLS Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 415-500 Price: $102.00
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HFLS Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 415K-500 Price: $111.00
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HFLS Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 415-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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HFLS-OA RNA, Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes-Osteoarthritis, adult Size: 10 ug CAT.#: 408OA-R10a Price: $350.00
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HFLS-OA RNA, Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes-Osteoarthritis, adult Size: 25 ug CAT.#: 408OA-R25a Price: $700.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Skeletal System Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Publications

2009Kubo, H., M. Shimizu, Y. Taya, T. Kawamoto, M. Michida, E. Kaneko, A. Igarashi, M. Nishimura, K. Segoshi, Y. Shimazu, K. Tsuji, T. Aoba, and Y. Kato. 2009. Identification of mesenchymal stem cell (MSC)-transcription factors by microarray and knockdown analyses, and signature molecule-marked MSC in bone marrow by immunohistochemistry. Genes to Cells. 14:407-424.2008Mototani, H., A. Iida, M. Nakajima, T. Furuichi, Y. Miyamoto, T. Tsunoda, A. Sudo, A. Kotani, A. Uchida, K. Ozaki, Y. Tanaka, Y. Nakamura, T. Tanaka, K. Notoya, and S. Ikegawa. 2008. A functional SNP in EDG2 increases susceptibility to knee osteoarthritis in Japanese. Human molecular genetics. 17:1790-1797.2006Lu, J., N. Zhi, S. Wong, and K.E. Brown. 2006. Activation of synoviocytes by the secreted phospholipase A2 motif in the VP1-unique region of parvovirus B19 minor capsid protein. Journal of Infectious Diseases. 193:582-590.