MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
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Instructions HGBF
Description
Human Gall Bladder Fibroblasts (HGBF) are derived from healthy human gall bladder. The gallbladder is located behind the liver, serves an important function in storing and enriching bile. HGBF from Cell Applications, Inc. provide an excellent model system to study gall bladder function and pathophysiology. Human Gall Bladder Fibroblasts are suitable for many cell-based assays including toxicity, drug screening and metabolism to study gall bladder function, physiology and related diseases.
HGBF are cultured in Gall Bladder Fibroblast Growth Medium and can propagate to 10 population doublings when cultured under the recommended conditions.
Characterization: Positive for fibroblast surface protein and fibronectin expression by ICC staining.
Human Gall Bladder Fibroblasts: HGBF Top: phase contrast, Bottom left: HGBF immunolabeled for fibroblast surface protein(green), Bottom right: fibronectin (green), nuclei are stained with DAPI.
Details
| Tissue | Normal healthy human gall bladder |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HGBF frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HGBF (704-05f), Growth Medium (7113-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, flasks or plates |
| Doublings | At least 10 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Gall Bladder Fibroblasts Cells Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 704K-05f | Price: $830.00 | |
| Cryopreserved Gall Bladder Fibroblasts Cells (HGBF), fetal: Frozen HGBF (5x10^5) | Size: 1 Cryovial | CAT.#: 704-05f | Price: $645.00 | |
| Proliferating Gall Bladder Fibroblasts Cells (HGBF), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 705-25f | Price: $645.00 | |
| Proliferating Gall Bladder Fibroblasts Cells (HGBF), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 705-75f | Price: $835.00 | |
| Proliferating Gall Bladder Fibroblasts Cells (HGBF), fetal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 705-24Wf | Price: $835.00 | |
| Proliferating Gall Bladder Fibroblasts Cells (HGBF), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 705-96Wf | Price: $955.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Gall Bladder Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 7113-GS | Price: $75.00 | |
| Human Gall Bladder Fibroblast Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 7113-500 | Price: $132.00 | |
| Human Gall Bladder Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 7113K-500 | Price: $145.00 | |
| Human Gall Bladder Fibroblast Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 7112-500 | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 |