MSDS Cryopreserved Cells
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Description
Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products.
HFDPC from Cell Applications, Inc. have been used to:
- Demonstrate that IL-1α affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF
- Investigate how gene expression and cell proliferation are affected by testosterone analogues, potential hair growth enhancing agents and microbial metabolites
- Study mechanisms of cellular senescence, and developed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT
- Discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1
(Click to Enlarge) Hair Follicle Dermal Papilla Cells, HFDPC (L) immunolabeled for vimentin (green) & nuclei stained w/ DAPI (blue) (R).
Details
Tissue | Normal human scalp hair follicle papilla |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HFDPC (2nd passage) in frozen in Basal Medium w/20% FBS & 5% DMSO |
Kit | Frzn HFDPC (602-05a), Gr Med (611-500), Subcltr Rgnt Kit (090K), Coll Coated T-75 Flask (125-75), Coll Soln (125-50) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 6 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Hair Follicle Dermal Papilla Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 602K-05a | Price: $1,035.00 | |
| Cryopreserved Hair Follicle Dermal Papilla Cells (HFDPC), adult: Frozen HFDPC (5x10^5) | Size: 1 Cryovial | CAT.#: 602-05a | Price: $775.00 | |
| Cryopreserved Human Hair Follicle Dermal Papilla Cells Total Kit, Top of Scalp, adult: 5x10^5 Cells (Top of Scalp, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 602tK-05a | Price: $1,094.00 | |
| Cryopreserved Hair Follicle Dermal Papilla Cells, Top of Scalp (HFDPCt), adult: Frozen HFDPC from Top of Scalp (5x10^5) | Size: 1 Cryovial | CAT.#: 602t-05a | Price: $834.00 | |
| Proliferating Hair Follicle Dermal Papilla Cells (HFDPC), adult: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: 603-25a | Price: $775.00 | |
| Proliferating Hair Follicle Dermal Papilla Cells (HFDPC), adult: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: 603-75a | Price: $965.00 | |
| Proliferating Hair Follicle Dermal Papilla Cells (HFDPC), adult: Actively growing, dividing cells, in medium | Size: 24 Well | CAT.#: 603-24Wa | Price: $965.00 | |
| Proliferating Hair Follicle Dermal Papilla Cells (HFDPC), adult: Actively growing, dividing cells, in medium | Size: 96 Well | CAT.#: 603-96Wa | Price: $1,085.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HFDPC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 611-500 | Price: $132.00 | |
| HFDPC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 611K-500 | Price: $150.00 | |
| HFDPC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 610-500 | Price: $81.00 | |
| HFDPC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 611-GS | Price: $108.00 | |
| Bovine Collagen Coating Solution: Bovine Collagen | Size: 50 ml | CAT.#: 125-50 | Price: $44.00 | |
| Bovine Collagen Coating Solution: Bovine Collagen | Size: 100 ml | CAT.#: 125-100 | Price: $86.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Dermal Follicle Papilla Cell RNA (HFDPC RNA), Adult: Total RNA prepared from Hair Follicle Dermal Papilla Cells, adult | Size: 10 ug | CAT.#: 602-R10a | Price: $398.00 | |
| Dermal Follicle Papilla Cell RNA (HFDPC RNA), Adult: Total RNA prepared from Hair Follicle Dermal Papilla Cells, adult | Size: 25 ug | CAT.#: 602-R25a | Price: $796.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| HFDPC Growth Medium wo FBS: Growth medium without FBS | Size: 500 ml | CAT.#: 611F-500 | Price: $156.00 | |
| HFDPC Growth Medium w/ 2% FBS: Growth medium with 2% FBS | Size: 500 ml | CAT.#: 611F2-500 | Price: $156.00 | |
| HFDPC Growth Medium wo FBS or Cyproterone: Growth medium without FBS and cyproterone | Size: 500 ml | CAT.#: 611FC-500 | Price: $156.00 |
Resources/Documents
Citations
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2017
Wang, Z., Y. Feng, L. Ma, X. Li, W. Ding and X. Chen. 2017. Hair growth promoting effect of white wax and policosanol from white wax on the mouse model of testosterone-induced hair loss. Biomed & Pharmacother, 89:438-446.
2013
Watabe, Y., M. Tomioka, A. Watabe, M. Aihara, S. Shimba, and H. Inoue. 2013. The clock gene brain and muscle Arnt-like protein-1 (BMAL1) is involved in hair growth. Archives of dermatological research. 305:755-761.
2012
Glynn, K., L. Duvel and D. Flower. 2012. Methods and compositions for modulating hair growth or regrowth. Patent US 8197865 B2.
Takigawa, H., and Y. Shibuya. 2012. The Metabolites of Food Microorganisms. Drug Discovery Research in Pharmacology. ISBN: 978-953-51-0213-7, InTech, DOI: 10.5772/33325.
2011
Suzuki, M., M. Kitagawa, S. Yamamoto, A. Sogabe, D. Kitamoto, T. Morita, T. Fukuoka, and T. Imura. 2011. Activator including biosurfactant as active ingredient, mannosyl erythritol lipid, and production method thereof. Patent Application US 20120070396 A1.
2010
Schönfelder, M., H. Michna, H. Hofmann, H.Meyer, M. Pfaffl, M. Reiter and K. Georgieva. 2010. Dopinganalytik – Möglichkeiten der Gen-Expressionserfassung aus Haarfollikelzellen, Lymphozyten und Mundschleimhautepithel. BISp-Jahrbuch – Forschungsförderung.
2008
Reiter, M., M.W. Pfaffl, M. Schonfelder, and H.H. Meyer. 2008. Gene expression in hair follicle dermal papilla cells after treatment with stanozolol. Biomarker insights. 4:1-8.
Sasajima, M., S. Moriwaki, M. Hotta, T. Kitahara, and Y. Takema. 2008. trans-3,4'-Dimethyl-3-hydroxyflavanone, a hair growth enhancing active component, decreases active transforming growth factor beta2 (TGF-beta2) through control of urokinase-type plasminogen activator (uPA) on the surface of keratinocytes. Biol. & Pharma. bulletin. 31:449-453.
2007
Haga, K., S.-i. Ohno, T. Yugawa, M. Narisawa-Saito, M. Fujita, M. Sakamoto, D.A. Galloway, and T. Kiyono. 2007. Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT. Cancer science. 98:147-154.
2006
Boivin, W.A., H. Jiang, O.B. Utting, and D.W.C. Hunt. 2006. Influence of interleukin-1α on androgen receptor expression and cytokine secretion by cultured human dermal papilla cells. Experimental dermatology. 15:784-793.
Cochran, S., B. Marshall, T. Barrows, Y. Su, and R. Schlicher. 2006. Hair grafts derived from plucked hair. Patent Application US 20070122387 A1.