MSDS Cryopreserved Cells
Instructions HFDPC
Cell Apps Flyer Skin Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles.  Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle.  HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products. 
HFDPC from Cell Applications, Inc. have been used to:
  • Demonstrate that IL-1α affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF
  • Investigate how gene expression and cell proliferation are affected by testosterone analogues, potential hair growth enhancing agents and microbial metabolites
  • Study mechanisms of cellular senescence, and developed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT
  • Discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1
(Click to Enlarge) Hair Follicle Dermal Papilla Cells, HFDPC (L) immunolabeled for vimentin (green) & nuclei stained w/ DAPI (blue) (R).

Details

Tissue
Normal human scalp hair follicle papilla
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HFDPC (2nd passage) in frozen in Basal Medium w/20% FBS & 5% DMSO
Kit
Frzn HFDPC (602-05a), Gr Med (611-500), Subcltr Rgnt Kit (090K), Coll Soln (125-50)
Proliferating 
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 6
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HFDPC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HFDPC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 602K-05a Price: $951.75
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Cryopreserved HFDPC Total Kit: 5x10^5 Cells (Top of Scalp, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 602tK-05a Price: $1,056.69
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Cryopreserved HFDPC, adult: Frozen HFDPC (5x10^5) Size: 1 Cryovial CAT.#: 602-05a Price: $726.47
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Cryopreserved HFDPC, adult: Frozen HFDPC from Top of Scalp (5x10^5) Size: 1 Cryovial CAT.#: 602t-05a Price: $834.09
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Proliferating HFDPC, adult: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: 603-25a Price: $726.47
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Proliferating HFDPC, adult: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: 603-75a Price: $930.96
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Proliferating HFDPC, adult: Actively growing, dividing cells, in medium Size: 6 Well CAT.#: 603-6Wa Price: $930.96
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Proliferating HFDPC, adult: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: 603-96Wa Price: $1,060.11
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Related Products

Product Size CAT.# Price Quantity
Human Hair Follicle Dermal Papilla Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 610-500 Price: $62.42
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Human Hair Follicle Dermal Papilla Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 611-500 Price: $121.62
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Human Hair Follicle Dermal Papilla Cell Growth Medium: Growth medium without FBS Size: 500 ml CAT.#: 611F-500 Price: $130.23
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Human Hair Follicle Dermal Papilla Cell Growth Medium: Growth medium with 2% FBS Size: 500 ml CAT.#: 611F2-500 Price: $130.23
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Human Hair Follicle Dermal Papilla Cell Growth Medium: Growth medium without FBS and cyproterone Size: 500 ml CAT.#: 611FC-500 Price: $130.23
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Human Hair Follicle Dermal Papilla Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 611K-500 Price: $130.23
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Human Hair Follicle Dermal Papilla Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 611K-500* Price: $127.00
Human Hair Follicle Dermal Papilla Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 611-GS Price: $67.80
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Extended Family Products

Product Size CAT.# Price Quantity
Collagen Coating Solution: Bovine Collagen Size: 50 ml CAT.#: 125-50 Price: $37.67
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Collagen Coating Solution: Bovine Collagen Size: 100 ml CAT.#: 125-100 Price: $75.34
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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HFDPC RNA: Total RNA prepared from Hair Follicle Dermal Papilla Cells, adult Size: 10 ug CAT.#: 602-R10a Price: $349.78
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HFDPC RNA: Total RNA prepared from Hair Follicle Dermal Papilla Cells, adult Size: 25 ug CAT.#: 602-R25a Price: $699.56
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

Cell Apps Flyer Skin Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Wang, Z., Y. Feng, L. Ma, X. Li, W. Ding and X. Chen. 2017. Hair growth promoting effect of white wax and policosanol from white wax on the mouse model of testosterone-induced hair lossBiomed & Pharmacother, 89:438-446.
2013
Watabe, Y., M. Tomioka, A. Watabe, M. Aihara, S. Shimba, and H. Inoue. 2013. The clock gene brain and muscle Arnt-like protein-1 (BMAL1) is involved in hair growth. Archives of dermatological research. 305:755-761.
2012
Glynn, K., L. Duvel and D. Flower. 2012. Methods and compositions for modulating hair growth or regrowth. Patent US 8197865 B2.
Takigawa, H., and Y. Shibuya. 2012. The Metabolites of Food Microorganisms. Drug Discovery Research in Pharmacology. ISBN: 978-953-51-0213-7, InTech, DOI: 10.5772/33325.
2011
Suzuki, M., M. Kitagawa, S. Yamamoto, A. Sogabe, D. Kitamoto, T. Morita, T. Fukuoka, and T. Imura. 2011. Activator including biosurfactant as active ingredient, mannosyl erythritol lipid, and production method thereof. Patent Application US 20120070396 A1.
2010
Schönfelder, M., H. Michna, H. Hofmann, H.Meyer, M. Pfaffl, M. Reiter and K. Georgieva. 2010. Dopinganalytik – Möglichkeiten der Gen-Expressionserfassung aus Haarfollikelzellen, Lymphozyten und Mundschleimhautepithel. BISp-Jahrbuch – Forschungsförderung.
2008
Reiter, M., M.W. Pfaffl, M. Schonfelder, and H.H. Meyer. 2008. Gene expression in hair follicle dermal papilla cells after treatment with stanozolol. Biomarker insights. 4:1-8.
Sasajima, M., S. Moriwaki, M. Hotta, T. Kitahara, and Y. Takema. 2008. trans-3,4'-Dimethyl-3-hydroxyflavanone, a hair growth enhancing active component, decreases active transforming growth factor beta2 (TGF-beta2) through control of urokinase-type plasminogen activator (uPA) on the surface of keratinocytes. Biol. & Pharma. bulletin. 31:449-453.
2007
Haga, K., S.-i. Ohno, T. Yugawa, M. Narisawa-Saito, M. Fujita, M. Sakamoto, D.A. Galloway, and T. Kiyono. 2007. Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT. Cancer science. 98:147-154.
2006
Boivin, W.A., H. Jiang, O.B. Utting, and D.W.C. Hunt. 2006. Influence of interleukin-1α on androgen receptor expression and cytokine secretion by cultured human dermal papilla cells. Experimental dermatology. 15:784-793.
 
Cochran, S., B. Marshall, T. Barrows, Y. Su, and R. Schlicher. 2006. Hair grafts derived from plucked hair. Patent Application US 20070122387 A1.