MSDS Cryopreserved Cells
Instructions HHF
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Hepatic Fibroblasts are one of the important cell type in the liver. HHF from Cell Applications, Inc. provide an excellent model system to study many aspects of human liver function, metabolism and pathophysiology. Human Hepatic Fibroblasts are primary cells derived from human liver and cultured in optimized Hepatic Fibroblast Growth Medium. HHF can propagate to 10 population doublings when cultured under the recommended conditions. Human Hepatic Fibroblasts are suitable for many cell-based assays including toxicity, drug screening and metabolism to study liver function, physiology and associated haptic diseases. 
 
Characterization: Positive for fibroblast surface protein expression by ICC staining.
Human Hepatic Fibroblasts: HHF Left: phase contrast, Right: HHF immunolabeled for FSP (green), nuclei are stained with PI.

Details

TissueNormal healthy human liver tissue
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HHF (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HHF (712-05f), Growth Medium (7111-500), Subcltr Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 2nd psg (flasks or plates)
DoublingsAt least 10
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

Instructions HHF

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cryopreserved HHF, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 712K-05f Price: $755.00
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Cryopreserved HHF, fetal: Frozen HHF (5x10^5) Size: 1 Cryovial CAT.#: 712-05f Price: $595.00
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Proliferating HHF, fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 713-25f Price: $595.00
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Proliferating HHF, fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 713-75f Price: $785.00
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Proliferating HHF, fetal: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 713-24Wf Price: $785.00
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Proliferating HHF, fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 713-96Wf Price: $905.00
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Related Products

Product Size CAT.# Price Quantity
Human Hepatic Fibroblast Basal Medium : Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: 7110-500 Price: $57.00
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Human Hepatic Fibroblast Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 7111-500 Price: $115.00
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Human Hepatic Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 7111K-500 Price: $126.00
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Human Hepatic Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 7111-GS Price: $58.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF103K Price: $404.00
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Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF103KS Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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