MSDS Cryopreserved Cells
Instructions HHF
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Hepatic Fibroblasts are one of the important cell type in the liver. HHF from Cell Applications, Inc. provide an excellent model system to study many aspects of human liver function, metabolism and pathophysiology. Human Hepatic Fibroblasts are primary cells derived from human liver and cultured in optimized Hepatic Fibroblast Growth Medium. HHF can propagate to 10 population doublings when cultured under the recommended conditions. Human Hepatic Fibroblasts are suitable for many cell-based assays including toxicity, drug screening and metabolism to study liver function, physiology and associated haptic diseases.
Characterization: Positive for fibroblast surface protein expression by ICC staining.
Human Hepatic Fibroblasts: HHF Left: phase contrast, Right: HHF immunolabeled for FSP (green), nuclei are stained with PI.
Details
| Tissue | Normal healthy human liver tissue |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HHF (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HHF (712-05f), Growth Medium (7111-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
| Doublings | At least 10 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Hepatic Fibroblasts Cells Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 712K-05f | Price: $860.00 | |
| Cryopreserved Hepatic Fibroblasts Cells (HHF), fetal: Frozen HHF (5x10^5) | Size: 1 Cryovial | CAT.#: 712-05f | Price: $675.00 | |
| Proliferating Hepatic Duct Fibroblasts Cells (HHF), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 713-25f | Price: $675.00 | |
| Proliferating Hepatic Duct Fibroblasts Cells (HHF), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 713-75f | Price: $865.00 | |
| Proliferating Hepatic Duct Fibroblasts Cells (HHF), fetal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 713-24Wf | Price: $865.00 | |
| Proliferating Hepatic Duct Fibroblasts Cells (HHF), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 713-96Wf | Price: $985.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Hepatic Duct Fibroblast Growth Medium : All-in-one ready-to-use | Size: 500 ml | CAT.#: 7111-500 | Price: $132.00 | |
| Human Hepatic Duct Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 7111K-500 | Price: $145.00 | |
| Human Hepatic Duct Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 7111-GS | Price: $75.00 | |
| Human Hepatic Duct Fibroblast Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 7110-500 | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 |
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