MSDS Cryopreserved Cells
Instructions HITASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Internal Thoracic Smooth Muscle Cells (HITASMC) are derived from the normal internal thoracic arteries (formerly called internal mammary arteries).
HITASMC, along with Human Coronary Artery Smooth Muscle Cells, both from Cell Applications, Inc. have been used to investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli.
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Internal Thoracic / Mammary Artery Smooth Muscle Cells: HITASMC.  Cells isolated from the vessel that supplies the anterior chest wall and breasts. 

Details

Tissue
Normal healthy human intrathoratic artery
QC
No bacteria, yeast, fungi, mycoplasma, virus 
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HITASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HITASMC (358-05a), Growth Medium, (311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 8
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HITASMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HITASMC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 358K-05a Price: $855.22
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Cryopreserved HITASMC, adult: Frozen HITASMC (5x10^5) Size: 1 Cryovial CAT.#: 358-05a Price: $709.25
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Proliferating HITASMC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 359-25a Price: $709.25
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Proliferating HITASMC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 359-75a Price: $952.48
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Proliferating HITASMC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 359-6Wa Price: $913.74
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Proliferating HITASMC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 359-96Wa Price: $1,042.89
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Related Products

Product Size CAT.# Price Quantity
Human Smooth Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.04
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Human Smooth Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.19
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Human Smooth Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.47
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Human Smooth Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.01
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Human Smooth Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.04
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-IL-6: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.38
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.21
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $53.76
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HITASMC RNA: Total RNA prepared from Human Internal Thoracic Artery Smooth Muscle Cells, adult Size: 10 ug CAT.#: 358-R10a Price: $349.78
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HITASMC RNA: Total RNA prepared from Human Internal Thoracic Artery Smooth Muscle Cells, adult Size: 25 ug CAT.#: 358-R25a Price: $699.56
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $138.57
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $521.98
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IL-6: Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.31
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IL-6: Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,174.94
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IL-6: Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $193.73
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IL-6 ELISA Kit, Human: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $516.60
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.10
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $532.74
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.43
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Publications

2014
Lange, M., T. Fujikawa, A. Koulova, S. Kang, M. Griffin, A. Lassaletta, A. Erat., E. Tobiash, C. Bianchi, N. Elmadhun, F. Selke, and A. Usheva. 2014. Arterial territory-specific phosphorylated retinoblastoma protein species and CDK2 promote differences in the vascular smooth muscle cell response to mitogens. Cell Cylcle, 13:315-323.
2013
Lange, M. 2013. Artery Specific Differences in Cell Cycle Regulation are Associated with Serum Induced Proliferation of Vascular Smooth Muscle Cells. MSc dissertation, University of Applied Science Bonn-Rhine-Sieg.
2011

Seymour, K., B. Sadowitz, J. Stein, J. Lawler, K. Maier, and V. Gahton. 2011. Vascular smooth muscle cell migration induced by domains of thrombospondin-1 is differentially regulated. Am J Surg, 202:553-557.