MSDS Cryopreserved Cells
Instructions HMEpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Mammary Epithelial Cells (HMEpC) provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to cancerogenesis.
HMEpC from Cell Applications, Inc. have been utilized in numerous research publications, for example to:
  • Investigate the role of exosomes secreted by cancer cells in formation of tumor permissive microenvironment through manipulation of normal mammary epithelium
  • Serve as control in a study investigating antitumor properties of cannabinoids and stem cell microenvironment
  • Determine that differential expression of glycoproteins allows classification of human breast cells into normal, benign, malignant, basal, and luminal groups
  • Identify ALDH isoform 5A1 as a potential target for treatment of human breast ductal carcinoma
  • Determine that combination of an anti-growth factor treatment using phototherapy (UV-B) is more effective
  • Investigate cell survival following DNA damage
  • Show the important roles of tumor suppressors in mammary epithelial differentiation
  • Explain protection of extracellular matrix from degradation in normal mammary epithelia,
  • Show that Maspin loss in metastatic cancer leads to unrestricted ECM degradation, contributing to metastasis, and that loss of EcSOD expression also promotes invasiveness by disrupting ECM
  • Investigate the role of shortened telomeres in initiation of genomic instability, cytokinesis failure and polyploidy
  • Elucidate the role of Myc in malignancy by studying its ability to transform primary epithelial cells
  • Demonstrate, along with Human dermal Fibroblasts, also from Cell Applications, Inc., that resveratrol inhibits mono-ubiquitination of histone H2B
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
(Click to Enlarge) Human Mammary Epithelial Cells (HMEPC) monolayer (A).  HMEpC immunolabeled for e-cadherin (B and D, green) and for cytokeratin 18 (C and D, red).  Nuclei are visualized with DAPI (D, blue).

Details

Tissue
Normal healthy human mammary glands
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth med
Cryovial
500,000 HMEpC (5th passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HMEpC(830-05a), Growth Med (815-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 6th psg (flasks or plates)
Doublings:
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HMEpC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HMEpC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 830K-05a Price: $785.00
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Cryopreserved HMEpC, Adult: Frozen HMEpC (5x10^5) Size: 1 Cryovial CAT.#: 830-05a Price: $630.00
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Proliferating HMEpC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 831-25a Price: $630.00
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Proliferating HMEpC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 831-75a Price: $834.00
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Proliferating HMEpC, Adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 831-24Wa Price: $834.00
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Proliferating HMEpC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 831-96Wa Price: $952.00
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Related Products

Product Size CAT.# Price Quantity
HMEpC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 814-500 Price: $62.00
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HMEpC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 815-500 Price: $110.00
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HMEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 815K-500 Price: $118.00
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HMEpC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 815-GS Price: $67.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $54.00
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Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $404.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HMEpC RNA, Adult: Total RNA prepared from Human Mammary Epithelial Cells, adult Size: 10 ug CAT.#: 830-R10a Price: $350.00
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HMEpC RNA, Adult: Total RNA prepared from Human Mammary Epithelial Cells, adult Size: 25 ug CAT.#: 830-R25a Price: $700.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 100 ug CAT.#: RP1026AF-100 Price: $95.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 500 ug CAT.#: RP1026AF-500 Price: $213.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 1000 ug CAT.#: RP1026AF-1000 Price: $290.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 100 ug CAT.#: RP1026-100 Price: $86.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 500 ug CAT.#: RP1026-500 Price: $194.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 1000 ug CAT.#: RP1026-1000 Price: $264.00
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Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit Size: 96 wells CAT.#: CL0389 Price: $484.00
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Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit Size: 96 wells CAT.#: CL0392 Price: $587.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 10 ug CAT.#: RP1173-10 Price: $194.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 100 ug CAT.#: RP1173-100 Price: $624.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 1000 ug CAT.#: RP1173-1000 Price: $5,327.00
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Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody Size: 100 ul CAT.#: CB19846 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Publications

2016
Pamarthy, S., L. Mao, G. Katara, S. Fleetwood, A. Kulshreshta, A. Gilman-Sachs and K. Beaman. 2016. The V-ATPase a2 isoform controls mammary gland development through Notch and TGF-β signaling. Cell Death & Dis, 7:e2443.
Pampalona, J., E. Roscioli, W. Silkworth, B. Bowden, A. Genesca, L. Tussell and D. Cimini. 2016. Chromosome Bridges Maintain Kinetochore-Microtubule Attachment throughout Mitosis and Rarely Break during Anaphase.  PLoS ONE 11(1):e0147420.
Armstrong, M., M. Stang, Y. Liu, J. Yan, E. Pizzoferrato, & J. Yim. 2015. IRF-1 inhibits NF-κB activity, suppresses TRAF2 and cIAP1 and induces breast cancer cell specific growth inhibition. Cancer Biology & Therapy,DOI:10.1080/15384047.2015.1046646.
Komatsu, N., Y. Fujita, M. Matsuda, and K. Aoki. 2015. mTORC1 upregulation via ERK-dependent gene expression change confers intrinsic resistance to MEK inhibitors in oncogenic KRas-mutant cancer cells. Oncogene , 23 February | doi:10.1038/onc.2015.16
2014
Khalkhali-Ellis, Z., W. Goossens, N. Margaryan, and M. Hendrix. 2014. Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland. PLoS ONE, July 23.
Teoh-Fitzgerald M, M. Fitzgerald, W. Zhong, R. Askeland, and F. Domann. 2014. Epigenetic reprogramming governs EcSOD expression during human mammary epithelial cell differentiation, tumorigenesis and metastasis. Oncogene, 33:358-368.
2013
Holtzhausen, A., C. Golzio, T. How, Y.-H. Lee, W.P. Schiemann, N. Katsanis, and G.C. Blobe. 2013. Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development. The FASEB Journal. 28:1-20.
Sarkar, S., S. Rajput, A.K. Tripathi, and M. Mandal. 2013. Targeted therapy against EGFR and VEGFR using ZD6474 enhances the therapeutic potential of UV-B phototherapy in breast cancer cells. Molecular cancer. 12:122.
Teoh-Fitzgerald, M., M. Fitzgerald, W. Zhong, R. Askeland, and F. Domann. 2013. Epigenetic reprogramming governs EcSOD expression during human mammary epithelial cell differentiation, tumorigenesis and metastasis. Oncogene.doi: 10.1038/onc.2012.582.
Timpe, L.C., R. Yen, N.V. Haste, C. Litsakos-Cheung, T.-Y. Yen, and B.A. Macher. 2013. Systemic alteration of cell-surface and secreted glycoprotein expression in malignant breast cancer cell lines. Glycobiology. 23:1240-1249.
Yen, T.-Y., S. Dutta, C. Litsakos-Cheung, A. Corona, L. Timpe, and B. Macher. 2013. Overcoming Challenges and Opening New Opportunities in Glycoproteomics. Biomolecules. 3:270-286.
2012
Hendrix, M., L. Postovit, R. Seftor, and E. Seftor. 2012. Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells. Patent US 8106004 B2.
Kaur, H., S. Mao, Q. Li, M. Sameni, S.A. Krawetz, B.F. Sloane, and R.R. Mattingly. 2012. RNA-Seq of Human Breast Ductal Carcinoma In Situ Models Reveals Aldehyde Dehydrogenase Isoform 5A1 as a Novel Potential Target. PloS one. 7:e50249.
Pampalona, J., C. Frías, A. Genescà, and L. Tusell. 2012. Progressive telomere dysfunction causes cytokinesis failure and leads to the accumulation of polyploid cells. PLoS genetics. 8:e1002679.
Teoh-Fitzgerald, M.L.T., M.P. Fitzgerald, T.J. Jensen, B.W. Futscher, and F.E. Domann. 2012. Genetic and Epigenetic Inactivation of Extracellular Superoxide Dismutase Promotes an Invasive Phenotype in Human Lung Cancer by Disrupting ECM Homeostasis. Molecular Cancer Research. 10:40-51.
2011
Gao, Z., M.S. Xu, T.L. Barnett, and C.W. Xu. 2011. Resveratrol induces cellular senescence with attenuated mono-ubiquitination of histone H2B in glioma cells. Biochemical and biophysical research communications. 407:271-276.
Hendrix, M., L. Postovit, R. Seftor, and E. Seftor. 2011. Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells. Patent Application US 20130102541 A1.
Yen, T.-Y., B.A. Macher, C.A. McDonald, C. Alleyne-Chin, and L.C. Timpe. 2011. Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of proteome research. 11:656-667.
2010
Hendrix, M., L. Postovit, R. Seftor, and E. Seftor. 2010. Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells. Patent Application US 20100273707 A1.
Lloyd, S. 2010. An Analysis of Estrogen Metabolism and Breast Cancer Risk. PhD Dissertation, U Pittsburgh.
Windhorst, S., R. Fliegert, C. Blechner, K. Möllmann, Z. Hosseini, T. Günther, M. Eiben, L. Chang, H. Lin, W. Fanick, U. Schumacher, B. Brandt and G. Mayr. 2010. Inositol 1,4,5-Trisphosphate 3-Kinase-A Is a New Cell Motility-promoting Protein That Increases the Metastatic Potential of Tumor Cells by Two Functional Activities. J Biol Chem, 285:5541-5554.
2009
Soler, D., J. Pampalona, L. Tusell, and A. Genescà. 2009. Radiation sensitivity increases with proliferation-associated telomere dysfunction in nontransformed human epithelial cells. Aging Cell. 8:414-425.
Thibodeaux, C., X. Liu, G. Disbrow, Y. Zhang, J. Rone, B. Haddad, and R. Schlegel. 2009. Immortalization and transformation of human mammary epithelial cells by a tumor-derived Myc mutant. Breast Cancer Res Treat. 116:281-294.
2008
Bailey, C.M., D.E. Abbott, N.V. Margaryan, Z. Khalkhali-Ellis, and M.J.C. Hendrix. 2008. Interferon Regulatory Factor 6 Promotes Cell Cycle Arrest and Is Regulated by the Proteasome in a Cell Cycle-Dependent Manner. Mol. Cell. Biol. 28:2235-2243.
Khalkhali-Ellis, Z., D.E. Abbott, C.M. Bailey, W. Goossens, N.V. Margaryan, S.L. Gluck, M. Reuveni, and M.J.C. Hendrix. 2008. IFN-γ regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells. J. Cell. Biochem. 105:208-218.
Postovit, L.-M., N.V. Margaryan, E.A. Seftor, D.A. Kirschmann, A. Lipavsky, W.W. Wheaton, D.E. Abbott, R.E.B. Seftor, and M.J.C. Hendrix. 2008. Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells. Proceedings of the National Academy of Sciences. 105:4329-4334.
Tusell, L., D. Soler, M. Agostini, J. Pampalona, and A. Genescà. 2008. The number of dysfunctional telomeres in a cell: one amplifies; more than one translocate. Cytogenetic and Genome Research. 122:315-325.
2007
Khalkhali-Ellis, Z., and M.J.C. Hendrix. 2007. Elucidating the Function of Secreted Maspin: Inhibiting Cathepsin D–Mediated Matrix Degradation. Cancer research. 67:3535-3539.
Wozniak, R., W. Klimecki, S. Lau, Y. Feinstein, and B. Futscher. 2007. 5-Aza-2'-deoxycytidine-mediated reductions in G9A histone methyltransferase and histone H3 K9 di-methylation levels are linked to tumor suppressor gene reactivation. Oncogene, 26:77-90.
2006
Ligresti, A., A.S. Moriello, K. Starowicz, I. Matias, S. Pisanti, L. De Petrocellis, C. Laezza, G. Portella, M. Bifulco, and V. Di Marzo. 2006. Antitumor activity of plant cannabinoids with emphasis on the effect of cannabidiol on human breast carcinoma. The Journal of pharmacology and experimental therapeutics. 318:1375-1387.
Solomon, J.M., R. Pasupuleti, L. Xu, T. McDonagh, R. Curtis, P.S. DiStefano, and L.J. Huber. 2006. Inhibition of SIRT1 Catalytic Activity Increases p53 Acetylation but Does Not Alter Cell Survival following DNA Damage. Mol. Cell. Biol. 26:28-38.