Instructions HMSC
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Mesenchymal Stem Cells (HMSC) can maintain multipotency and proliferate extensively in vitro, providing new avenues for cell-based therapy in the restoration of damaged or diseased tissue. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages.
Read about HMSC in "Mesenchymal Stem Cells - Global Strategic Business Report," ID: 2769226, September 2016, by Global Industry Analysts, Inc
HMSC from Cell Applications, Inc. have been utilized to investigate
  • Effects of TGF-β1 on differentiation toward different lineages, and show that it inhibits adipogenic differentiation and induces either chondrogenic, osteogenic or smooth muscle cell differentiation, depending the specific culture conditions
  • The differentiation potential of stem cells
  • HLA expression profiles of bone marrow-derived and adipose-derived stem cells
  • Heart valve-shaped tri-layered collagen-based constructs for stem cell seeding and differentiation to use in heart valve tissue engineering
(Click to Enlarge) Human Mesenchymal Stem Cells (HMSC) (A) differentiating into multiple lineages.  Adipogenic - Oil Red O staining (B), Chondrogenic - Alcian Blue Staining (C) and Osteogenic - Alzarin Red S staining (D).

Details

Tissue
Normal healthy human bone marrow
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Bone mineralization in Osteoblast Differentiation Med, Lipid accumulation in Adipocyte Diff Medium 
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HMSC frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HMSC (492-05f), Growth Medium (419-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HMSC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HMSC Total Kit: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 492K-05f Price: $846.82
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Cryopreserved HMSC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 492K-05a Price: $867.81
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Cryopreserved HMSC, adult: Frozen HMSC (5x10^5) Size: 1 Cryovial CAT.#: 492-05a Price: $704.94
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Cryopreserved HMSC, fetal: Frozen HMSC (5x10^5) Size: 1 Cryovial CAT.#: 492-05f Price: $683.42
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Proliferating HMSC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 493-25a Price: $704.94
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Proliferating HMSC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 493-75a Price: $909.43
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Proliferating HMSC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 493-6Wa Price: $909.43
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Proliferating HMSC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 493-96Wa Price: $1,038.58
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Proliferating HMSC, fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 493-25f Price: $683.42
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Proliferating HMSC, fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 493-75f Price: $887.91
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Proliferating HMSC, fetal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 493-6Wf Price: $887.91
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Proliferating HMSC, fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 493-96Wf Price: $1,017.06
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Related Products

Product Size CAT.# Price Quantity
Human Mesenchymal Stem Cell Basal Medium: Basal HMSC medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 418-500 Price: $62.42
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Human Mesenchymal Stem Cell Basal Medium: Serum-Free Basal HMSC Medium Size: 500 ml CAT.#: 418sf-500 Price: $102.24
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Human Mesenchymal Stem Cell Basal Medium, Xeno-Free: Xeno-Free Basal HMSC Medium Size: 500 ml CAT.#: 418XF-500 Price: $113.01
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Human Mesenchymal Stem Cell Growth Medium: HMSC Growth Medium without Antibiotics Size: 500 ml CAT.#: 419A-500 Price: $121.62
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Human Mesenchymal Stem Cell Growth Medium: Serum-Free HSMC Growth Medium Size: 500 ml CAT.#: 419sf-500 Price: $182.96
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Human Mesenchymal Stem Cell Growth Medium: All-in-one ready-to-use HMSC Growth Medium Size: 500 ml CAT.#: 419-500 Price: $121.62
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Human Mesenchymal Stem Cell Growth Medium Kit: Basal HSMC medium & HSMC growth supplement sold together packaged separately Size: yields 500 ml CAT.#: 419K-500 Price: $130.23
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Human Mesenchymal Stem Cell Growth Medium Kit: Serum-Free HMSC Growth Medium Kit Size: Yields 500 ml CAT.#: 419sfK-500 Price: $188.34
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Human Mesenchymal Stem Cell Growth Medium Kit, Xeno-Free: Xeno-Free HMSC Growth Medium Kit Size: Yields 500 ml CAT.#: 419XFK-500 Price: $209.87
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Human Mesenchymal Stem Cell Growth Medium, Xeno-Free: Xeno-Free HMSC Growth Medium Size: 500 ml CAT.#: 419XF-500 Price: $204.49
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Human Mesenchymal Stem Cell Growth Supplement: Serum-Free HMSC Growth Supplement Size: 5 ml CAT.#: 419sf-GS Price: $107.63
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Human Mesenchymal Stem Cell Growth Supplement: Added to HMSC Basal Medium to create HMSC Growth Medium Size: 50 ml CAT.#: 419-GS Price: $67.80
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Human Mesenchymal Stem Cell Growth Supplement, Xeno-Free: Xeno-Free HMSC Growth Supplement Size: 5 ml CAT.#: 419XF-GS Price: $118.39
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-SCF: Rabbit Stem Cell Factor Antibody Size: 100 ul CAT.#: CG1536 Price: $325.00
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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HMSC RNA: Total RNA prepared from Human Human Marrow Stromal Cells, adult Size: 10 ug CAT.#: 492-R10a Price: $408.98
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HMSC RNA: Total RNA prepared from Human Human Marrow Stromal Cells, adult Size: 25 ug CAT.#: 492-R25a Price: $817.95
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HMSC RNA: Total RNA prepared from Human Human Marrow Stromal Cells, fetal Size: 10 ug CAT.#: 492-R10f Price: $408.98
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HMSC RNA: Total RNA prepared from Human Human Marrow Stromal Cells, fetal Size: 25 ug CAT.#: 492-R25f Price: $817.95
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Human Adipocyte Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes Size: 250 ml CAT.#: 811D-250 Price: $94.71
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Human Osteoblast Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 417D-250 Price: $79.64
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IL-7: Human Interleukin-7 Size: 10 ug CAT.#: RP1011-10 Price: $193.73
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IL-7: Human Interleukin-7 Size: 100 ug CAT.#: RP1011-100 Price: $624.23
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IL-7: Human Interleukin-7 Size: 1000 ug CAT.#: RP1011-1000 Price: $5,327.44
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IL-7 ELISA Kit, Human: Human Interleukin-7 ELISA Kit Size: 96 Wells CAT.#: CL0779 Price: $586.56
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M-CSF: Human Macrophage-Colony Stimulating Factor Size: 10 ug CAT.#: RP1003-10 Price: $193.73
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M-CSF: Human Macrophage-Colony Stimulating Factor Size: 100 ug CAT.#: RP1003-100 Price: $624.23
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M-CSF: Human Macrophage-Colony Stimulating Factor Size: 1000 ug CAT.#: RP1003-1000 Price: $5,327.44
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M-CSF ELISA Kit, Human: Human Macrophage-Colony Stimulating Factor ELISA Kit Size: 96 Wells CAT.#: CL0444 Price: $511.22
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M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free Size: 10 ug CAT.#: RP1003AF-10 Price: $213.10
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M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free Size: 100 ug CAT.#: RP1003AF-100 Price: $686.65
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M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free Size: 1000 ug CAT.#: RP1003AF-1000 Price: $5,860.18
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MIP-3β / CCL19: Human Macrophage Inflammatory Protein-3 beta Size: 20 ug CAT.#: RP1135-20 Price: $193.73
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MIP-3β / CCL19: Human Macrophage Inflammatory Protein-3 beta Size: 100 ug CAT.#: RP1135-100 Price: $484.31
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MIP-3β / CCL19: Human Macrophage Inflammatory Protein-3 beta Size: 1000 ug CAT.#: RP1135-1000 Price: $3,174.94
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SCF: Human Stem Cell Factor Size: 10 ug CAT.#: RP1004-10 Price: $193.73
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SCF: Human Stem Cell Factor Size: 100 ug CAT.#: RP1004-100 Price: $484.31
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SCF: Human Stem Cell Factor Size: 1000 ug CAT.#: RP1004-1000 Price: $3,174.94
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SCF, Animal-Free: Human Stem Cell Factor, Animal-Free Size: 1000 ug CAT.#: RP1004AF-1000 Price: $3,492.43
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SCF, Animal-Free: Human Stem Cell Factor, Animal-Free Size: 100 ug CAT.#: RP1004AF-100 Price: $532.74
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SCF, Animal-Free: Human Stem Cell Factor, Animal-Free Size: 10 ug CAT.#: RP1004AF-10 Price: $213.10
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SDF-1α / CXCL12: Human Stromal Cell-Derived Factor-1-alpha Size: 10 ug CAT.#: RP1020-10 Price: $193.73
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SDF-1α / CXCL12: Human Stromal Cell-Derived Factor-1-alpha Size: 100 ug CAT.#: RP1020-100 Price: $484.31
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SDF-1α / CXCL12: Human Stromal Cell-Derived Factor-1-alpha Size: 1000 ug CAT.#: RP1020-1000 Price: $3,174.94
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SDF-1β / CXCL12: Human Stromal Cell-Derived Factor-1-beta Size: 10 ug CAT.#: RP1159-10 Price: $193.73
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SDF-1β / CXCL12: Human Stromal Cell-Derived Factor-1-beta Size: 100 ug CAT.#: RP1159-100 Price: $484.31
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SDF-1β / CXCL12: Human Stromal Cell-Derived Factor-1-beta Size: 1000 ug CAT.#: RP1159-1000 Price: $3,174.94
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

Cell Apps Flyer Skeletal System Cells

Format: PDF

Downoad Now
5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now

Publications

2016
Bellayr, I., R. Marklein, J. Lo Surdo, S. Bauer and R. Puri. 2016. Identification of Predictive Gene Markers for Multipotent Stromal Cell Proliferation. Stem Cells & Dev, 25:861-873.
2017
2016
Bellayr, I., R. Marklein, J. Lo Surdo, S. Bauer and R. Puri. 2016. Identification of Predictive Gene Markers for Multipotent Stromal Cell ProliferationStem Cells & Dev, 25:861-873.
Tan, C., H. Shichinohe, Z. Wang, S. Hamauchi, T. Abumiya, N. Nakayama, K. Kazumata, T. Ito, K. Kudo, S. Takamoto and K. Houkin. 2016. Feasibility and efficiency of human bone marrow stromal cell culture with allogeneic platelet lysate-supplementation for cell therapy against stroke.Stem Cells Intl, 6104780.
2015
Teng, N., Y. Liu, H. Wu, Y. Liu, J. Ho, and O. Lee. 2015. Promotion of Mesenchymal-to-Epithelial Transition by Rac1 Inhibition with Small Molecules Accelerates Hepatic Differentiation of Mesenchymal Stromal Cells. Tissue Engineering Part A.  21:1444-1454.
2014
Chang, H., D. Docheva, U. Knothe and M. Tate. 2014. Arthritic Periosteal Tissue From Joint Replacement Surgery: A Novel, Autologous Source of Stem Cells. Stem Cells & Regen Med, 3:308-317.
Gadkari, R., L. Zhao, T. Teklemariam, and B. Hantash. 2014. Human embryonic stem cell derived-mesenchymal stem cells: an alternative mesenchymal stem cell source for regenerative medicine therapy. Regenerative Medicine, 9:453-465.
Matsubara, Y., T. Zama, Y. Ikeda, Y. Uruga, T. Suda and S. Matsuoka. 2014. Method for producing megakaryocytes, platelets and/or thrombopoietin using mesenchymal cells. Patent Application US 20160177265 A1.
Purandare, B., T. Teklemariam, L. Zhao, and B. Hantash. 2014. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow-and adipose-derived mesenchymal stem cells. Regenerative Medicine 9: 67-79.
Teklemariam, T., B. Purandare, L. Zhao, and B. Hantash. 2014. Inhibition of DNA methylation enhances HLA-G expression in human mesenchymal stem cells. BBRC 452:753-759.
2013
Purandare, B., T. Teklemariam, L. Zhao, and B.M. Hantash. 2013. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow- and adipose-derived mesenchymal stem cells. Regenerative Medicine. 9:67-79.
2012
Kato, Y., I. Hirata, and M. Kanawa. 2012. Animal Cell Culture Kit, Method for Culturing Animal Cells, Method for Selective Culture of Animal Cells and Cell Differentiation Method. Patent Application US 20140030804 A1.
2011
Hirota, Y., and M. Hakoda. 2011. Relationship between dielectric characteristic by DEP levitation and differentiation activity for stem cells. Key Engineering Materials. 459:84-91.
Park, J., H. Yoon, B. Yoo, Y. Kim, and Y. Shin. 2011. Method for the preparation of dermal papilla tissue employing mesenchymal stem cells. Patent US 8017390 B2.
Tedder, M.E., A. Simionescu, J. Chen, J. Liao, and D.T. Simionescu. 2011. Assembly and testing of stem cell-seeded layered collagen constructs for heart valve tissue engineering. Tissue engineering. Part A. 17:25-36.
Zhao, L., and B.M. Hantash. 2011. TGF-b1 Regulates Differentiation of Bone Marrow Mesenchymal Stem Cells. Vitamins and hormones 87:127-141.
2010
Tedder, M.E.B. 2010. Characterization and Evaluation of a Novel Tissue Engineered Aortic Heart Valve Construct. Clemson University, PhD dissertation.
Tedder, M., A. Simionescu, J. Chen, J. Liao, and D. Simionescu. 2010. Assembly and Testing of Stem Cell-Seeded Layered Collagen Constructs for Heart Valve Tissue Engineering. Tissue Engineering Part A., 17:25-36.
2006
Baron, U., I. Turbachova, A. Hellwag, F. Eckardt, K. Berlin, U. Hoffmüller, P. Gardina, and S. Olek. 2006.  DNA Methylation Analysis as a Tool for Cell Typing. Epigenetics 1:55-60.
Le Visage, C., S.W. Kim, K. Tateno, A.N. Sieber, J.P. Kostuik, and K.W. Leong. 2006. Interaction of human mesenchymal stem cells with disc cells: changes in extracellular matrix biosynthesis. Spine. 31:2036-2042.