MSDS Cryopreserved Cells
Instructions HOb-OA
Cell Apps Flyer Skeletal System Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Description
Human Osteoblasts-Osteoarthritis (HOb-OA) are isolated from bone of patients with osteoarthritis (OA) and provide an excellent model system to study the pathophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.
Characterization: Positive for bone mineralization.
Details
Tissue | Bone from donors w/ osteoarthritis |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | 500,000 HOb-OA (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HOb-OA (406OA-05), Growth Medium (417-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Cryopreserved Human Osteoblasts Osteo Arthritis (HOb-OA) Total Kit, adult: 5x10^5 Cells (from donor with Osteoarthitis, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 406OAK-05a | Price: $1,111.00 | |
Cryopreserved Osteoblasts, Osteoarthritis (HOb-OA), adult: Frozen HOb-OA (5x10^5) | Size: 1 Cryovial | CAT.#: 406OA-05a | Price: $925.00 | |
Proliferating Osteoblasts, Osteoarthritis (HOb-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: T-25 Flask | CAT.#: 407OA-25a | Price: $925.00 | |
Proliferating Osteoblasts, Osteoarthritis (HOb-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: T-75 Flask | CAT.#: 407OA-75a | Price: $1,115.00 | |
Proliferating Osteoblasts, Osteoarthritis (HOb-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: 24 Well | CAT.#: 407OA-24Wa | Price: $1,115.00 | |
Proliferating Osteoblasts, Osteoarthritis (HOb-OA), adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | Size: 96 Well | CAT.#: 407OA-96Wa | Price: $1,235.00 |
Related Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
HOb Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 416-500 | Price: $81.00 | |
HOb Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 417D-250 | Price: $138.00 | |
HOb Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 417-500 | Price: $133.00 | |
HOb Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 417K-500 | Price: $144.00 | |
HOb Growth Medium, wo FBS: Growth medium without FBS | Size: 500 ml | CAT.#: 417F-500 | Price: $144.00 | |
HOb Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 417-GS | Price: $81.00 | |
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Extended Family Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Monoclonal Bone Morphogenic Protein 4, BMP-4, Antibody: Monoclonal Bone Morphogenic Protein 4, BMP-4, Antibody | Size: 100 ul | CAT.#: CB12470 | Price: $302.00 | |
Mouse BMPR2 Antibody: Mouse BMPR2 Antibody | Size: 100 ul | CAT.#: CP10368 | Price: $302.00 | |
Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 | Size: 10 ug | CAT.#: RP1053-10 | Price: $194.00 | |
Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 | Size: 100 ug | CAT.#: RP1053-100 | Price: $624.00 | |
Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 | Size: 1000 ug | CAT.#: RP1053-1000 | Price: $5,327.00 | |
Human BMP-2 ELISA Kit: Human Bone Morphogenic Protein-2 ELISA Kit | Size: 96 Wells | CAT.#: CL0311 | Price: $500.00 | |
Human BMP-4 ELISA Kit: Human Bone Morphogenic Protein-4 ELISA Kit | Size: 96 Wells | CAT.#: CL0314 | Price: $457.00 | |
Human BMP-5 ELISA Kit: Human Bone Morphogenic Protein-5 ELISA Kit | Size: 96 Wells | CAT.#: CL0310 | Price: $500.00 | |
Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | Size: 20 ug | CAT.#: RP1060-20 | Price: $194.00 | |
Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | Size: 100 ug | CAT.#: RP1060-100 | Price: $484.00 | |
Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | Size: 1000 ug | CAT.#: RP1060-1000 | Price: $4,090.00 | |
Osteoblast RNA (HOb RNA), Adult: Total RNA prepared from Human Osteoblasts, adult | Size: 10 ug | CAT.#: 406-R10a | Price: $514.00 | |
Osteoblast RNA (HOb RNA), Adult: Total RNA prepared from Human Osteoblasts, adult | Size: 25 ug | CAT.#: 406-R25a | Price: $1,028.00 | |
Osteoblast RNA (HOb RNA), Fetal: Total RNA prepared from Human Osteoblasts, fetal | Size: 25 ug | CAT.#: 406-R25f | Price: $796.00 | |
Osteoblast RNA (HOb RNA), Re-Differentiated, Fetal: Total RNA prepared from Human Osteoblasts, Differentiated, fetal | Size: 10 ug | CAT.#: 406D-R10f | Price: $465.00 | |
Osteoblast RNA (HOb RNA), Re-Differentiated, Fetal: Total RNA prepared from Human Osteoblasts, Differentiated, fetal | Size: 25 ug | CAT.#: 406D-R25f | Price: $930.00 | |
Osteoblast RNA (HOb RNA), Stimulated, Fetal: Total RNA prepared from Human Osteoblasts, Stimulated, fetal | Size: 10 ug | CAT.#: 406S-R10f | Price: $514.00 | |
Osteoblast RNA (HOb RNA), Stimulated, Fetal: Total RNA prepared from Human Osteoblasts, Stimulated, fetal | Size: 25 ug | CAT.#: 406S-R25f | Price: $1,028.00 | |
Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand | Size: 10 ug | CAT.#: RP1019-10 | Price: $194.00 | |
Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand | Size: 100 ug | CAT.#: RP1019-100 | Price: $624.00 | |
Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand | Size: 1000 ug | CAT.#: RP1019-1000 | Price: $5,327.00 | |
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free | Size: 10 ug | CAT.#: RP1019AF-10 | Price: $213.00 | |
Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free | Size: 100 ug | CAT.#: RP1019AF-100 | Price: $687.00 | |
Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free | Size: 1000 ug | CAT.#: RP1019AF-1000 | Price: $5,860.00 |
Resources/Documents
Citations
Publications
2006
Ando, T., J. Ichikawa, M. Wako, K. Hatsushika, Y. Watanabe, M. Sakuma, K. Tasaka, H. Ogawa, Y. Hamada, H. Yagita, and A. Nakao. 2006. TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells. Arthritis research & therapy. 8:R146.