MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Cell Apps Fllyer Cardiovascular Cells
Instructions HPcF
Description
Human Pericardial Fibroblasts (HPcF) are the most prevalent cell type in the heart. HPcF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology. HPcF from Cell Applications, Inc. are derived from human heart. HPcF can propagate to 10 population doublings when cultured in Pericardial Fibroblast Growth Medium under the recommended conditions and provide an excellent model system to study heart function and cardiovascular diseases.
Characterization: Positive or fibroblast surface protein by ICC staining.
Top: Human Pericardial Fibroblasts (HPcF). Bottom: HPcF stained for fibroblast surface protein (in green) and DAPI (in blue).
Details
| Tissue | Normal healthy human heart tissue |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HPcF (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HPcF (310-05f), Growth Medium (3111-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
| Doublings | At least 16 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Proliferating Pericardial Fibroblasts (HPcF), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 311-25f | Price: $685.00 | |
| Proliferating Pericardial Fibroblasts (HPcF), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 311-75f | Price: $875.00 | |
| Proliferating Pericardial Fibroblasts (HPcF), fetal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 311-24Wf | Price: $875.00 | |
| Proliferating Pericardial Fibroblasts (HPcF), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 311-96Wf | Price: $995.00 | |
| Cryopreserved Pericardial Fibroblasts (HPcF), fetal: Frozen HPcF (5x10^5) | Size: 1 Cryovial | CAT.#: 310-05f | Price: $685.00 | |
| Cryopreserved Human Pericardial Fibroblasts Cells Total Kit, adult: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 310K-05f | Price: $870.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Pericardial Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 3111-GS | Price: $79.00 | |
| Pericardial Fibroblast Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 3111-500 | Price: $132.00 | |
| Pericardial Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 3111K-500 | Price: $144.00 | |
| Pericardial Fibroblast Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 3110-500 | Price: $70.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 |