Instructions HPBB
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description

Human Peripheral Blood B Cells (HPBB) provide a useful tool for studying various aspects of pathology and biology of human B lymphocytes in vitro. We isolate HPBB from the blood of healthy donors and cryopreserve them immediately after isolation.  These are the antibody forming cells of the immune system and originate from pluripotent hematopoetic stem cells and differentiate in the fetal liver. In adults, B cells principally originate in the bone marrow. Newly generated, surface Ig-expressing mature B cells leave the bone marrow and circulate into peripheral lymphoid organs.
(Click to Enlarge) Human Peripheral Blood CD19+ B Cells: HPBB.  Antibody-producing lymphocytes.

Details

Tissue
Normal healthy human peripheral blood
QCNo bacteria, yeast, fungi, mycoplasma or virus
CharacterCD19 positive selection
Cryovial
2,000,000 HPBB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
Kit
Cryovial Frozen HPBB (6904-20a), Maintenance Medium (615-250)
Cultured Cells
Shipped in Transfer Medium (flasks or plates)
Doublings
N/A - Blood cells don't proliferate in vitro
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPBB

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HPBB, Adult: 2x10^6 CD19+ B Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6904K-20a Price: $577.00
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Cryopreserved HPBB, Adult: Frozen HPBB, CD19+ (2x10^6) Size: 1 Cryovial CAT.#: 6904-20a Price: $537.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: T-25 Flask CAT.#: 6905-25a Price: $537.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: T-75 Flask CAT.#: 6905-75a Price: $742.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: 6 Well CAT.#: 6905-6Wa Price: $742.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: 96 Well CAT.#: 6905-96Wa Price: $871.00
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Related Products

Product Size CAT.# Price Quantity
Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $65.00
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Human Blood Cell Basal Medium: Basal medium (contains no growth supplement).  Add Supplement before use. Size: 250 ml CAT.#: 614-250 Price: $38.00
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Human Blood Cell Culture Medium: Optimized and ready for use. Size: 250 ml CAT.#: 615-250 Price: $66.00
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Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Size: Yields 250 ml CAT.#: 615K-250 Price: $76.00
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Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium Size: 40 ml CAT.#: 615-CS Price: $40.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $65.00
-+
Human B cell Activating Factor (BAFF/BLyS): Human B cell Activating Factor Size: 20 ug CAT.#: RP1024-20 Price: $194.00
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Human B cell Activating Factor (BAFF/BLyS): Human B cell Activating Factor Size: 150 ug CAT.#: RP1024-150 Price: $624.00
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Human B cell Activating Factor (BAFF/BLyS): Human B cell Activating Factor Size: 1000 ug CAT.#: RP1024-1000 Price: $5,327.00
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Human BAFF ELISA Kit: Human B Cell Activating Factor ELISA Kit Size: 96 Wells CAT.#: CL0663 Price: $533.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 10 ug CAT.#: RP1021AF-10 Price: $213.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 100 ug CAT.#: RP1021AF-100 Price: $533.00
-+
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 1000 ug CAT.#: RP1021AF-1000 Price: $4,499.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 10 ug CAT.#: RP1021-10 Price: $194.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 100 ug CAT.#: RP1021-100 Price: $484.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 1000 ug CAT.#: RP1021-1000 Price: $4,090.00
-+
Human L-Selectin ELISA Kit: Human L-Selectin ELISA Kit Size: 96 wells CAT.#: CL0503 Price: $500.00
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Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha Size: 20 ug CAT.#: RP1147-20 Price: $194.00
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Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha Size: 100 ug CAT.#: RP1147-100 Price: $484.00
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Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha Size: 1000 ug CAT.#: RP1147-1000 Price: $3,175.00
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Human MIP-3a ELISA Kit: Human Macrophage Inflammatory Protein-3 alpha ELISA Kit Size: 96 Wells CAT.#: CL0453 Price: $495.00
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Mouse BAFF ELISA Kit: Mouse B Cell Activating Factor ELISA Size: 96 Wells CAT.#: CL0664 Price: $619.00
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Mouse Epstein-Barr Virus Induced Gene 3 (EBI3 subunit / IL-27 / IL-35): Mouse EBV Induced Gene-3 Size: 10 ug CAT.#: RP2057-10 Price: $194.00
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Mouse Epstein-Barr Virus Induced Gene 3 (EBI3 subunit / IL-27 / IL-35): Mouse EBV Induced Gene-3 Size: 100 ug CAT.#: RP2057-100 Price: $624.00
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Mouse Epstein-Barr Virus Induced Gene 3 (EBI3 subunit / IL-27 / IL-35): Mouse EBV Induced Gene-3 Size: 1000 ug CAT.#: RP2057-1000 Price: $5,327.00
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Rabbit B Cell Activating Factor Receptor Anitbody: Rabbit B Cell Activating Factor Receptor Anitbody Size: 100 ul CAT.#: CA1391 Price: $302.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications


2016
Teramura, Y., S. Asif, K. Ekdahl, E. Gustafson and B. Nilsson. 2016. Cell adhesion induced using surface modification with cell-penetrating peptide-conjugated PEG-lipid: A new cell glue for 3D cell-based structures. ACS Appl Mater Interfaces, DOI: 10.1021/acsami.6b14584.
2011
Yen, T.-Y., B.A. Macher, C.A. McDonald, C. Alleyne-Chin, and L.C. Timpe. 2011. Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of proteome research. 11:656-667.