Instructions HPAd
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Adipocytes
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Description

Human Preadipocytes (HPAd) Pre-Screened 
HPAd are prescreened for Adipogenesis Signaling:  Primary cultures of adipocytes are prepared by inducing differentiation of human primary preadipocytes and are prescreened for the expression of the following major adipocyte markers:
  • PPARγ (Peroxisome proliferator-activated receptor gamma), an adipocyte-specific nuclear hormone receptor
  • C/EBPα (CCAAT/enhancer binding protein alpha), a transcription factor involved in creating and maintaining the adipocyte phenotype
  • IR (Insulin receptor), which plays a critical role in induction of adipocyte differentiation, as well as functional regulation
  • ACCα (Acetyl-CoA carboxylase alpha), a key enzyme for de novo fatty acid synthesis and lipogenic capability of adipocytes
  • GSK-3β (Glycogen synthase kinase-3-beta), a ubiquitous kinase implicated in regulation of adipocyte gene expression, signaling, adipogenesis, insulin action and glycogen synthesis
  • Adiponectin, a bioactive adipokine specifically secreted by differentiated adipocyte and involved in metabolic regulation
 
(Cllick to Enlarge) Human Adipocytes (HAd) (differentiated) accumulate lipid droplets visualized by Oil Red O (A)Western blot analysis of undifferentiated human pre-adipocytes (lane 1) and differentiated mature adipocytes (lane 2) lysates (B, C) Western blot analysis of the unstimulated (lane 1) and insulin-stimulated (lane 2) differentiated human adipocytes using anti-phospho-insulin receptor antibodies (left blot) and regular anti-insulin receptor antibodies (right blot) (D).

Details

(Detail for Figure) Human Preadipocytes: HPAd: Pre-Screened. High level expression of both PPARg and C/EBPa proteins was detected in our prescreened human adipocytes but not in the undifferentiated preadipocytes (Fig. B).  Additionally, IR expression is dramatically increased in the prescreened human adipocytes as compared to that in undifferentiated preadipocytes. There was also a slight increase in Akt expression in differentiated adipocytes.  Our results further demonstrate higher expression levels for both metabolic enzymes, ACCa and GSK-3b, in the mature adipocytes compared to the undifferentiated preadipocytes.  Adiponectin was also detected only in the mature adipocytes, but not in the undifferentiated preadipocytes (Fig. C). Insulin is not only critical to adipocyte differentiation, but also serves as a key metabolic regulator.  Thus, insulin response is a characteristic assay for adipocyte function.  We confirmed that the pre-screened adipocytes respond strongly to insulin stimulation (Fig. D).  Strong phosphorilation of Tyr 1165/6 of insulin receptor was detected in the insulin-stimulated (lane 2), but not in untreated adipocytes (lane 1).  Total insulin receptor levels did not change before and after insulin stimulation (right blot).
Instructions HPAd

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Instructions HPAd

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Instructions HPAd

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HPAd, Subcutaneous, Pre-Screened, Adult: 5x10^5 Cells (From Subcutaneous Tissue, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S802sK-05a Price: $463.00
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Cryopreserved HPAd, Subcutaneous, Pre-Screened, Adult: Frozen HPAd from subcutaneous tissue (5x10^5) Size: 1 Cryovial CAT.#: S802s-05a Price: $361.00
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Proliferating HPAd, Subcutaneous, Pre-Screened, Adult: Actively growing, dividing cells from subcutaneous tissue, in medium, adult Size: T-25 Flask CAT.#: S803s-25a Price: $361.00
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Proliferating HPAd, Subcutaneous, Pre-Screened, Adult: Actively growing, dividing cells from subcutaneous tissue, in medium, adult Size: T-75 Flask CAT.#: S803s-75a Price: $565.00
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Proliferating HPAd, Subcutaneous, Pre-Screened, Adult: Actively growing, dividing cells from subcutaneous tissue, in medium, adult Size: 6 Well CAT.#: S803s-6Wa Price: $565.00
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Proliferating HPAd, Subcutaneous, Pre-Screened, Adult: Actively growing, dividing cells from subcutaneous tissue, in medium, adult Size: 96 Well CAT.#: S803s-96Wa Price: $694.00
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Related Products

Product Size CAT.# Price Quantity
HAd Maintenance Medium: Maintenance medium for adipocytes Size: 250 ml CAT.#: 811M-250 Price: $68.00
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HPAd Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 810-500 Price: $77.00
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HPAd Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes Size: 250 ml CAT.#: 811D-250 Price: $95.00
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HPAd Differentiation Medium, Component Kit: Differentiation medium components sold together packaged separately Size: Yields 250 ml CAT.#: 811DS-250 Price: $132.00
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HPAd Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 811-500 Price: $135.00
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HPAd Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 811K-500 Price: $148.00
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HPAd Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 811-GS Price: $59.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Preadipocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF802K Price: $350.00
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Cytofect™ Preadipocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF802KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HAd Maintenance Medium wo Insulin: Maintenance Medium for adipocytes without insulin Size: 250 ml CAT.#: 811Mi-250 Price: $78.00
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HAd Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 811S-250 Price: $62.00
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HPAd-S RNA, Adult: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult Size: 10 ug CAT.#: 802s-R10a Price: $350.00
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HPAd-S RNA, Adult: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult Size: 25 ug CAT.#: 802s-R25a Price: $700.00
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Human Leptin: Human Leptin Size: 200 ug CAT.#: RP1037-200 Price: $86.00
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Human Leptin: Human Leptin Size: 1000 ug CAT.#: RP1037-1000 Price: $194.00
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Human Leptin: Human Leptin Size: 5000 ug CAT.#: RP1037-5000 Price: $538.00
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Human Leptin ELISA Kit: Human Leptin ELISA Kit Size: 96 wells CAT.#: CL0437 Price: $587.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Adipocytes

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Cell Apps Poster Primary Cells

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Cell Applications Inc Brochure

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