MSDS Cryopreserved Cells
Instructions HPAEC
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells

Description

Human Pulmonary Artery Endothelial Cells (HPAEC) provide an excellent model system to study many aspects of cardiovascular function and disease, including diabetes-associated complications, mechanisms of endothelial dysfunction caused by environment, oxidative stress, hypoxia and inflammation, as well as mode of action of various natural compounds and drug candidates.
Note that several publications describe the differences between endothelial cells from different vascular beds and between macro- and micro-vascular cells from the same organ, highlighting the importance of confirming any new findings on cell lots obtained from several different origins.  Cell Applications, Inc. offers the widest variety of endothelial cells, and is well equipped to meet this need.
HPAEC from Cell Applications have been used to investigate or demonstrate
  • Vasculoprotective effects of propofol, demonstrating its potential for treating pulmonary arterial hypertension
  • Hits from a high-throughput screening assay and discover anziaic acid inhibits bacterial activity
  • Endothelin-1 is released by endothelial cells during acute crisis in sickle cell anemia, increases vascular constriction
  • Pollutant particles cause vascular dysfunction by up-regulating clotting-related genes
  • Central role of AMP-activated protein kinase in normal endothelial barrier function can be disrupted, causing endothelial hyperpermeability and lung injury
  • Anti-inflammatory and cardioprotective effects of resveratrol
  • cGMP prevents oxidant-induced damage to the endothelial barrier function
  • Radiation causes endothelial cell senescence due to up-regulation of proliferative signaling in the presence of cell cycle arrest
  • Vanadium exposure causes pulmonary vasoconstriction mediated in part by the inhibition of endothelial NO production
  • Cytotoxic effects of a novel pore-forming protein, proposed as an anti-tumor agent
  • Effects of Bone Morphogenic Protein-4, helping to explain why its upregulation leads to atherosclerosis and hypertension
  • Macro-vascular pulmonary endothelial cells accumulate HIF-1 under hypoxic conditions
  • Transportation of bioactive lipids and its silencing by siRNA
 
 
 
 
 
 
 
 
 
 
 
 
(Click to Enlarge) Human Pulmonary Artery Endothelial Cells: HPAEC (A).  HPAEC immunolabeled for CD31/PECAM (Cat# CB13678); nuclei stained with PI (red) (B). HPAEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (C,D).

Details

Tissue
Normal healthy human pulmonary artery 
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
VIII-related Ag expression, DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HPAEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HPAEC (302-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPAEC

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Instructions HPAEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HPAEC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 302K-05a Price: $853.00
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Cryopreserved HPAEC, Adult: Frozen HPAEC (5x10^5) Size: 1 Cryovial CAT.#: 302-05a Price: $695.00
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Cryopreserved HPAEC-AS, Pre-Scr, Adult: Frozen HPAEC-AS from donor with Asthma (5x10^5) Size: 1 Cryovial CAT.#: S302AS-05a Price: $851.00
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Cryopreserved HPAEC-AS, Pre-Scr, Adult: 5x10^5 Cells (from donor with Asthma, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S302ASK-05a Price: $1,004.00
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Proliferating HPAEC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 303-25a Price: $695.00
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Proliferating HPAEC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 303-75a Price: $885.00
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Proliferating HPAEC, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 303-6Wa Price: $885.00
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Proliferating HPAEC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 303-96Wa Price: $1,005.00
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Related Products

Product Size CAT.# Price Quantity
Human EC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 210-500 Price: $70.00
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Human EC Basal Medium wo Phenol Red: Basal medium without growth supplement and phenol red Size: 500 ml CAT.#: 210PR-500 Price: $75.00
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Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211-500 Price: $105.00
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Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: Yields 500 ml CAT.#: 211K-500 Price: $124.00
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Human EC Growth Medium wo Antibiotics: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211A-500 Price: $113.00
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Human EC Growth Medium wo FBS: Growth medium without FBS. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211F-500 Price: $113.00
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Human EC Growth Medium wo Hydrocortisone: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211H-500 Price: $113.00
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Human EC Growth Medium wo Phenol Red: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 500 ml CAT.#: 211PR-500 Price: $113.00
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Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. Size: 15 ml CAT.#: 211-GS Price: $57.00
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Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics Size: 15 ml CAT.#: 211A-GS Price: $68.00
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Human EC Growth Supplement wo FBS: Growth supplement without FBS Size: 5 ml CAT.#: 211F-GS Price: $70.00
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Human EC Growth Supplement wo Hydrocortisone: Growth supplement without hydrocortisone Size: 15 ml CAT.#: 211H-GS Price: $68.00
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Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in 100% confluent culture. Not for cell growth. Size: 500 ml CAT.#: 113-500 Price: $113.00
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Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. Size: 500 ml CAT.#: 113PR-500 Price: $118.00
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Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 209-250 Price: $70.00
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Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red Size: 250 ml CAT.#: 209PR-250 Price: $79.00
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Human ECl Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. Size: 500 ml CAT.#: 213-500 Price: $123.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HPAEC RNA, Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult Size: 10ug CAT.#: 302-R10a Price: $350.00
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HPAEC RNA, Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult Size: 25ug CAT.#: 302-R25a Price: $700.00
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Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $587.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $139.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $522.00
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Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.00
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Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $517.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $194.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,090.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $533.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,499.00
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Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.00
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Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody Size: 100 ul CAT.#: CA1080 Price: $302.00
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Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.00
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Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Airway Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Publications

2016
2015
2013
Cheng, B., S. Cao, V. Vasquez, T. Annamalai, G. Tamayo-Castillo, J. Clardy, and Y.-C. Tse-Dinh. 2013. Identification of Anziaic Acid, a Lichen Depside from Hypotrachyna sp., as a New Topoisomerase Poison Inhibitor. PloS one. 8:e60770.
Panganiban, R.A.M., and R.M. Day. 2013. Inhibition of IGF-1R Prevents Ionizing Radiation-Induced Primary Endothelial Cell Senescence. PloS one. 8:e78589.
Xing, J., Q. Wang, K. Coughlan, B. Viollet, C. Moriasi, and M.-H. Zou. 2013. Inhibition of AMP-Activated Protein Kinase Accentuates Lipopolysaccharide-Induced Lung Endothelial Barrier Dysfunction and Lung Injury in Vivo. Am. J. pathology. 182:1021-1030.
2012
Cao, L., L. Xu, B. Huang, and L. Wu. 2012. Propofol Increases Angiotensin-Converting Enzyme 2 Expression in Human Pulmonary Artery Endothelial Cells. Pharmacology. 90:342-347.
Hisano, Y., N. Kobayashi, A. Yamaguchi, and T. Nishi. 2012. Mouse SPNS2 Functions as a Sphingosine-1-Phosphate Transporter in Vascular Endothelial Cells. PloS one. 7:e38941.
2011
Yang, C.J., C.Y. Lin, T.-c. Hsieh, S.C. Olson, and J.M. Wu. 2011. Control of eotaxin-1 expression and release by resveratrol and its metabolites in culture human pulmonary artery endothelial cells. American journal of cardiovascular disease. 1:16.
2007
Karoly, E.D., Z. Li, L.A. Dailey, X. Hyseni, and Y.-C.T. Huang. 2007. Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure. Environmental health perspectives. 115:535.
2006
Fujiuchi, S., Y. Yamazaki, Y. Fujita, Y. Nishigaki, A. Taked, Y. Yamamoto, T. Fijikane, T. Shimizu, S. Osanai, T. Takahashi, and K. Kikuchi. 2006. S-Nitrosoglutathione (SNOG) Accumulates Hypoxia Inducible Factor-1α in Main Pulmonary Artery Endothelial Cells but not in Micro Pulmonary Vessel Endothelial Cells. In THE ARTERIAL CHEMORECEPTORS. Vol. 580. Y. Hayashida, C. Gonzalez, and H. Kondo, editors. Springer US. 63-71.
Li, Z., X. Hyseni, J.D. Carter, J.M. Soukup, L.A. Dailey, and Y.-C.T. Huang. 2006. Pollutant particles enhanced H2O2 production from NAD (P) H oxidase and mitochondria in human pulmonary artery endothelial cells. American Journal of Physiology-Cell Physiology. 291:C357-C365.
Moldobaeva, A., L.E. Welsh-Servinsky, L.A. Shimoda, R.S. Stephens, A.D. Verin, R.M. Tuder, and D.B. Pearse. 2006. Role of protein kinase G in barrier-protective effects of cGMP in human pulmonary artery endothelial cells. Am. J. Physiol. 290:L919-L930.
2005
Li, Z., J. Carter, L. Dailey, and Y. Huang. 2005. Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type 1 Receptor. Environmental Health Perspectives, 113:1009.
2004
Ergul, S., C.Y. Brunson, J. Hutchinson, A. Tawfik, A. Kutlar, R.C. Webb, and A. Ergul. 2004. Vasoactive factors in sickle cell disease: In vitro evidence for endothelin-1-mediated vasoconstriction. American Journal of Hematology. 76:245-251.

Li, Z., J.D. Carter, L.A. Dailey, and Y.C. Huang. 2004. Vanadyl sulfate inhibits NO production via threonine phosphorylation of eNOS. Environmental health perspectives. 112:201-206.