MSDS Cryopreserved Cells
Instructions HPAEC
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Description
Human Pulmonary Artery Endothelial Cells (HPAEC) provide an excellent model system to study many aspects of cardiovascular function and disease, including diabetes-associated complications, mechanisms of endothelial dysfunction caused by environment, oxidative stress, hypoxia and inflammation, as well as mode of action of various natural compounds and drug candidates.
Note that several publications describe the differences between endothelial cells from different vascular beds and between macro- and micro-vascular cells from the same organ, highlighting the importance of confirming any new findings on cell lots obtained from several different origins. Cell Applications, Inc. offers the widest variety of endothelial cells, and is well equipped to meet this need.
HPAEC from Cell Applications have been used to investigate or demonstrate
- Vasculoprotective effects of propofol, demonstrating its potential for treating pulmonary arterial hypertension
- Hits from a high-throughput screening assay and discover anziaic acid inhibits bacterial activity
- Endothelin-1 is released by endothelial cells during acute crisis in sickle cell anemia, increases vascular constriction
- Pollutant particles cause vascular dysfunction by up-regulating clotting-related genes
- Central role of AMP-activated protein kinase in normal endothelial barrier function can be disrupted, causing endothelial hyperpermeability and lung injury
- Anti-inflammatory and cardioprotective effects of resveratrol
- cGMP prevents oxidant-induced damage to the endothelial barrier function
- Radiation causes endothelial cell senescence due to up-regulation of proliferative signaling in the presence of cell cycle arrest
- Vanadium exposure causes pulmonary vasoconstriction mediated in part by the inhibition of endothelial NO production
- Cytotoxic effects of a novel pore-forming protein, proposed as an anti-tumor agent
- Effects of Bone Morphogenic Protein-4, helping to explain why its upregulation leads to atherosclerosis and hypertension
- Macro-vascular pulmonary endothelial cells accumulate HIF-1 under hypoxic conditions
- Transportation of bioactive lipids and its silencing by siRNA
(Click to Enlarge) Human Pulmonary Artery Endothelial Cells: HPAEC (A). HPAEC immunolabeled for CD31/PECAM (Cat# CB13678); nuclei stained with PI (red) (B). HPAEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (C,D).
Details
Tissue | Normal healthy human pulmonary artery |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | VIII-related Ag expression, DiI-Ac-LDL uptake |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HPAEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HPAEC (302-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 15 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Pulmonary Artery Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 302K-05a | Price: $914.00 | |
| Cryopreserved Pulmonary Artery Endothelial Cells (HPAEC), adult: Frozen HPAEC (5x10^5) | Size: 1 Cryovial | CAT.#: 302-05a | Price: $730.00 | |
| Cryopreserved Pulmonary Artery Endothelial Cells, Asthma (HPAEC-AS), Pre-Screened, adult: Frozen HPAEC-AS from donor with Asthma (5x10^5) | Size: 1 Cryovial | CAT.#: S302AS-05a | Price: $945.00 | |
| Cryopreserved Pre-Screened Human Pulmonary Artery Endothelial Cells with Asthma Total Kit: 5x10^5 Cells (from donor with Asthma, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S302ASK-05a | Price: $1,119.00 | |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 303-25a | Price: $730.00 | |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 303-75a | Price: $920.00 | |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 303-24Wa | Price: $920.00 | |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 303-96Wa | Price: $1,040.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211-500 | Price: $121.00 | |
| Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: Yields 500 ml | CAT.#: 211K-500 | Price: $143.00 | |
| Human EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 210-500 | Price: $91.00 | |
| Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 15 ml | CAT.#: 211-GS | Price: $74.00 | |
| Human EC Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. | Size: 500 ml | CAT.#: 213-500 | Price: $141.00 | |
| Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | Size: 250 ml | CAT.#: 209-250 | Price: $81.00 | |
| Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red | Size: 250 ml | CAT.#: 209PR-250 | Price: $91.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody | Size: 100 ul | CAT.#: CA1080 | Price: $302.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Pulmonary Artery Endothelial Cell RNA (HPAEC RNA), Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult | Size: 10ug | CAT.#: 302-R10a | Price: $398.00 | |
| Pulmonary Artery Endothelial Cell RNA (HPAEC RNA), Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult | Size: 25ug | CAT.#: 302-R25a | Price: $796.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2016
Day, R. 2016. Methods of Inhibiting IGF-1R Activation or Downtream Signalling Thereof to Reduce Radiation-Induced Cellular Senescence. Patent Application US 20160000744 A1.
2015
Zelko, I. and R. Folz. 2015. Regulation of Oxidative Stress in Pulmonary Artery Endothelium. Modulation of Extracellular Superoxide Dismutase and NOX4 Expression Using Histone Deacetylase Class I Inhibitors. Am J Resp Cell & Molec Biol, 53:513-524.
2013
Cheng, B., S. Cao, V. Vasquez, T. Annamalai, G. Tamayo-Castillo, J. Clardy, and Y.-C. Tse-Dinh. 2013. Identification of Anziaic Acid, a Lichen Depside from Hypotrachyna sp., as a New Topoisomerase Poison Inhibitor. PloS one. 8:e60770.
Panganiban, R.A.M., and R.M. Day. 2013. Inhibition of IGF-1R Prevents Ionizing Radiation-Induced Primary Endothelial Cell Senescence. PloS one. 8:e78589.
Xing, J., Q. Wang, K. Coughlan, B. Viollet, C. Moriasi, and M.-H. Zou. 2013. Inhibition of AMP-Activated Protein Kinase Accentuates Lipopolysaccharide-Induced Lung Endothelial Barrier Dysfunction and Lung Injury in Vivo. Am. J. pathology. 182:1021-1030.
2012
Cao, L., L. Xu, B. Huang, and L. Wu. 2012. Propofol Increases Angiotensin-Converting Enzyme 2 Expression in Human Pulmonary Artery Endothelial Cells. Pharmacology. 90:342-347.
Hisano, Y., N. Kobayashi, A. Yamaguchi, and T. Nishi. 2012. Mouse SPNS2 Functions as a Sphingosine-1-Phosphate Transporter in Vascular Endothelial Cells. PloS one. 7:e38941.
2011
Yang, C.J., C.Y. Lin, T.-c. Hsieh, S.C. Olson, and J.M. Wu. 2011. Control of eotaxin-1 expression and release by resveratrol and its metabolites in culture human pulmonary artery endothelial cells. American journal of cardiovascular disease. 1:16.
2007
Karoly, E.D., Z. Li, L.A. Dailey, X. Hyseni, and Y.-C.T. Huang. 2007. Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure. Environmental health perspectives. 115:535.
2006
Fujiuchi, S., Y. Yamazaki, Y. Fujita, Y. Nishigaki, A. Taked, Y. Yamamoto, T. Fijikane, T. Shimizu, S. Osanai, T. Takahashi, and K. Kikuchi. 2006. S-Nitrosoglutathione (SNOG) Accumulates Hypoxia Inducible Factor-1α in Main Pulmonary Artery Endothelial Cells but not in Micro Pulmonary Vessel Endothelial Cells. In THE ARTERIAL CHEMORECEPTORS. Vol. 580. Y. Hayashida, C. Gonzalez, and H. Kondo, editors. Springer US. 63-71.
Li, Z., X. Hyseni, J.D. Carter, J.M. Soukup, L.A. Dailey, and Y.-C.T. Huang. 2006. Pollutant particles enhanced H2O2 production from NAD (P) H oxidase and mitochondria in human pulmonary artery endothelial cells. American Journal of Physiology-Cell Physiology. 291:C357-C365.
Moldobaeva, A., L.E. Welsh-Servinsky, L.A. Shimoda, R.S. Stephens, A.D. Verin, R.M. Tuder, and D.B. Pearse. 2006. Role of protein kinase G in barrier-protective effects of cGMP in human pulmonary artery endothelial cells. Am. J. Physiol. 290:L919-L930.
2005
Li, Z., J. Carter, L. Dailey, and Y. Huang. 2005. Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type 1 Receptor. Environmental Health Perspectives, 113:1009.
2004
Ergul, S., C.Y. Brunson, J. Hutchinson, A. Tawfik, A. Kutlar, R.C. Webb, and A. Ergul. 2004. Vasoactive factors in sickle cell disease: In vitro evidence for endothelin-1-mediated vasoconstriction. American Journal of Hematology. 76:245-251.
Li, Z., J.D. Carter, L.A. Dailey, and Y.C. Huang. 2004. Vanadyl sulfate inhibits NO production via threonine phosphorylation of eNOS. Environmental health perspectives. 112:201-206.