MSDS Cryopreserved Cells
Instructions HPASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Airway Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) from Cell Applications, Inc. have been used to show that:
  • Antitumor drugs can selectively target remodeled pulmonary vessels, but not normal vessels
  • Serotonin induces their growth and activates the BMP receptor
  • IL-22 promotes the growth of pulmonary vascular SMCs via a signaling mechanism that involves NADPH oxidase-dependent oxidation
  • Inducers of pulmonary hypertension, characterized by thickened pulmonary arterial walls, can be suppressed by targeting gene transcription
  • Cell growth can be suppressed by trans-retinoic acid by inducing expression of a known cell growth suppressor,
  • Retinoic acid and its receptors may be involved in cell growth and pulmonary vascular remodeling
  • Iron chelation exerts effects on vascular remodeling and is implicated for development of pulmonary hypertension as a result of ROS activity
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Pulmonary Artery Smooth Muscle Cells: HPASMC.  Cells isolated from the vessel that carries deoxygenated blood from the heart to the lungs.

Details

Tissue
Normal healthy human pulmonary artery 
QC
No bacteria, yeast, fungi, mycoplasma, virus 
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HPASMC frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HPASMC (352-05a), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPASMC

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved HPASMC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 352K-05a Price: $814.29
-+
Cryopreserved HPASMC, adult: Frozen HPASMC (5x10^5) Size: 1 Cryovial CAT.#: 352-05a Price: $667.28
-+
Proliferating HPASMC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 353-25a Price: $667.28
-+
Proliferating HPASMC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 353-75a Price: $871.76
-+
Proliferating HPASMC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 353-6W Price: $871.76
-+
Proliferating HPASMC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 353-96W Price: $1,000.91
-+

Related Products

Product Size CAT.# Price Quantity
Human Smooth Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.04
-+
Human Smooth Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.19
-+
Human Smooth Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.47
-+
Human Smooth Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.01
-+
Human Smooth Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.04
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
-+

Extended Family Products

Product Size CAT.# Price Quantity
Anti-IL-6: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.38
-+
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
-+
Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
-+
Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.21
-+
Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $53.76
-+
HPASMC RNA: Total RNA prepared from Human Pulmonary Artery Smooth Muscle Cells, adult Size: 10 ug CAT.#: 352-R10a Price: $349.78
-+
HPASMC RNA: Total RNA prepared from Human Pulmonary Artery Smooth Muscle Cells, adult Size: 25 ug CAT.#: 352-R25a Price: $699.56
-+
Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $138.57
-+
Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $521.98
-+
IL-6: Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.31
-+
IL-6: Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,174.94
-+
IL-6: Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $193.73
-+
IL-6 ELISA Kit, Human: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $516.60
-+
IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.10
-+
IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $532.74
-+
IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.43
-+

Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

Downoad Now
Cell Apps Flyer Cardiovascular Cells

Format: PDF

Downoad Now
Cell Apps Flyer Airway Cells

Format: PDF

Downoad Now
5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now

Publications

2016
Sahoo, S., D. Meijles, I. Ghouleh, M. Tandon, E. Cifuentes-Pagano, J. Sembrat, M. Rojas, E. Goncharova and P. Pagano. 2016. MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension. PLoS ONE, 11(5):e013780.
2015
Zisman, L. 2015. Therapeutic Indications of Kinase InhibitorsPatent Application 20150352111 A1.
2014
Ibrahim, Y., C. Wong, L. Pavlickova, L. Liu, L. Trasar, G. Bansal, and Y. Suzuki. 2014. Mechanism of the Susceptibility of Remodeled Pulmonary Vessels to DrugInduced Cell Killing. J Am Heart Assoc, 3: e000520. Feb 26.
Zisman, L. 2014. Non-selective kinase inhibitors. Patent Application US 20150353527 A1.
2013
Bansal, G., D. Das, C.-Y. Hsieh, Y.-H. Wang, B.A. Gilmore, C.-M. Wong, and Y.J. Suzuki. 2013. IL-22 activates oxidant signaling in pulmonary vascular smooth muscle cells. Cellular signalling. 25:2727-2733.
2012
Bansal, G., C. Wong, L. Liu and Y. Suzuki. 2012. Oxidant signaling for interleukin-13 gene expression in lung smooth muscle cells. Free Rad Biol & Med, 52:1552-1559.
Wong, C., I. Preston, N. Hill and Y. Suzuki. 2012. Iron chelation inhibits the development of pulmonary vascular remodeling. Free Rad Biol & Med, 53:1738-1747.
2009
Day, R.M., I.A. Matus, Y.J. Suzuki, K.J. YEUM, J. Qin, A.M. PARK, V. Jain, T. Kuru, and G. Tang. 2009. Plasma levels of retinoids, carotenoids and tocopherols in patients with mild obstructive sleep apnoea. Respirology. 14:1134-1142.
Liu, Y., W. Ren, R. Warburton, D. Toksoz, and B.L. Fanburg. 2009. Serotonin induces Rho/ROCK-dependent activation of Smads 1/5/8 in pulmonary artery smooth muscle cells. The FASEB Journal. 23:2299-2306.
2007
Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.
2006
Behera, A.K., E. Hildebrand, J. Szafranski, H.H. Hung, A.J. Grodzinsky, R. Lafyatis, A.E. Koch, R. Kalish, G. Perides, and A.C. Steere. 2006. Role of aggrecanase 1 in Lyme arthritis. Arthritis & Rheumatism. 54:3319-3329.
Day, R.M., Y.H. Lee, A.M. Park, and Y.J. Suzuki. 2006. Retinoic acid inhibits airway smooth muscle cell migration. American journal of respiratory cell and molecular biology. 34:695-703.
2005
Preston, I.R., G. Tang, J.U. Tilan, N.S. Hill, and Y.J. Suzuki. 2005. Retinoids and pulmonary hypertension. Circulation. 111:782-790.