Instructions HRGEpC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Renal Glomerular Epithelial Cells (HRGEpC) have received increasing attention lately as researchers work to better understand their pathophysiology. Active investigations suggest HRGEpC may serve as a selective permeability barrier to urinary filtrate. Other studies indicate functions related to mechanosensing and contraction.
As a network of capillaries at the beginning of the kidney nephron, the glomerulus filters blood carried out by the nephron during urine formation. The blood plasma is filtered by passing through the glomerular membrane, consisting of the capillary wall and the epithelial layer of Bowman's capsule. This glomerular capillary tuft is supported by a matrix of collagen and surrounded by an envelope of epithelial cells.
While the precise role of HRGEpC in disease remains unclear, glomerular problems are a leading cause of kidney disease. When activated, HRGEpC show elevated proliferation, migration and ECM production, activities possibly tied to glomerulonephritis, diabetic nephropathy and other pathologies.
HRGEpC are rigorously tested for their ability to attach and spread on tissue culture ware and proliferate in RenaEpi Growth Medium.
Characterization: HRGEpC stain positive for Nephrin, pan-CK and Vimentin expression by ICC staining
(Click to Enlarge) Top: Human Renal Glomerular Epithelial Cells (HRGEpC). Bottom left: HRGEpC stained for vimentin (in green) and DAPI (in blue). Bottom center: HRGEpC stained for pan-cytokeratin (in green) and DAPI (in blue). Bottom right: HRGEpC stained for nephrin (in red) and DAPI (in blue).
Details
| Tissue | Normal healthy human kidney |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Character | Nephrin, pan-CK, Vimentin positive |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HRGEpC (2nd passage) frozen in Basal Med w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HRGEpC (942-05n), Gr Med (911-500), Subculture Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
| Doublings | 2-3 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Renal Glomerular Epithelial Cells (HRGEpC), neonatal: Frozen HRGEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 942-05n | Price: $745.00 | |
| Proliferating Renal Glomerular Epithelial Cells (HRGEpC), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 943-25n | Price: $745.00 | |
| Proliferating Renal Glomerular Epithelial Cells (HRGEpC), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 943-75n | Price: $935.00 | |
| Cryopreserved Human Renal Glomerular Epithelial Cells Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 942K-05n | Price: $941.00 | |
| Proliferating Renal Glomerular Epithelial Cells (HRGEpC), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 943-96Wn | Price: $1,055.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human RenaEpi Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 910-500 | Price: $85.00 | |
| Human RenaEpi Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 911-500 | Price: $143.00 | |
| Human RenaEpi Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 911K-500 | Price: $155.00 | |
| Human RenaEpi Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 10 ml | CAT.#: 911-GS | Price: $91.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
| Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
| Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 50 ug | CAT.#: 1H50-50 | Price: $158.00 | |
| Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 250 ug | CAT.#: 1H50-250 | Price: $595.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
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