Instructions HRGEpC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
Cell Apps Poster Primary Cells
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Description

Human Renal Glomerular Epithelial Cells (HRGEpC) have received increasing attention lately as researchers work to better understand their pathophysiology.  Active investigations suggest HRGEpC may serve as a selective permeability barrier to urinary filtrate.  Other studies indicate functions related to mechanosensing and contraction.
As a network of capillaries at the beginning of the kidney nephron, the glomerulus filters blood carried out by the nephron during urine formation. The blood plasma is filtered by passing through the glomerular membrane, consisting of the capillary wall and the epithelial layer of Bowman's capsule. This glomerular capillary tuft is supported by a matrix of collagen and surrounded by an envelope of epithelial cells.
While the precise role of HRGEpC in disease remains unclear, glomerular problems are a leading cause of kidney disease.  When activated, HRGEpC show elevated proliferation, migration and ECM production, activities possibly tied to glomerulonephritis, diabetic nephropathy and other pathologies.
HRGEpC are rigorously tested for their ability to attach and spread on tissue culture ware and proliferate in RenaEpi Growth Medium.
Characterization: HRGEpC stain positive for Nephrin, pan-CK and Vimentin expression by ICC staining
(Click to Enlarge) Top: Human Renal Glomerular Epithelial Cells (HRGEpC). Bottom left: HRGEpC stained for vimentin (in green) and DAPI (in blue). Bottom center: HRGEpC stained for pan-cytokeratin (in green) and DAPI (in blue). Bottom right: HRGEpC stained for nephrin (in red) and DAPI (in blue).

Details

TissueNormal healthy human kidney
QCNo bacteria, yeast, fungi, mycoplasma, virus
CharacterNephrin, pan-CK, Vimentin positive
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HRGEpC (2nd passage) frozen in Basal Med w/ 10% FBS, 10% DMSO
KitCryovial frozen HRGEpC (942-05n), Gr Med (911-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 3rd psg (flasks or plates)
Doublings2-3
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HRGEpC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HRGEpC, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 942K-05n Price: $887.00
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Cryopreserved HRGEpC, Neonatal: Frozen HRGEpC (5x10^5) Size: 1 Cryovial CAT.#: 942-05n Price: $712.00
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Proliferating HRGEpC, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 943-25n Price: $712.00
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Proliferating HRGEpC, Neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 943-6Wn Price: $928.00
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Proliferating HRGEpC, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 943-75n Price: $928.00
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Proliferating HRGEpC, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 943-96Wn Price: $1,066.00
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Related Products

Product Size CAT.# Price Quantity
Human RenaEpi Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 910-500 Price: $65.00
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Human RenaEpi Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 911-500 Price: $124.00
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Human RenaEpi Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 911K-500 Price: $135.00
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Human RenaEpi Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml (3 parts) CAT.#: 911-GS Price: $70.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $404.00
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 50 ug CAT.#: 1H50-50 Price: $139.00
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 250 ug CAT.#: 1H50-250 Price: $522.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Epithelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now