Instructions HSkMC Normal
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal Muscle Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Skeletal Muscle Cells (HSkMC), isolated from adult or fetal donor limbs are positive for sarcomere myosin and can undergo differentiation to exhibit actin and myosin myofilaments.  Our HSkMC provide a useful model system to study many aspects of muscular function and disease.  Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, so select lots undergo an additional set of Pre-Screening at Cell Applications, Inc. to demonstrate AMPK signaling and responsiveness to insulin stimulation.
 
HSkMC from Cell Applications, Inc. have been used to:
  • Serve as a differentiated control in a study of developmental regulator genes in hESC
  • Characterize statin-induced gene expression changes and demonstrate cytotoxic effect of statins in skeletal muscle cells
  • Identify molecular mechanisms of mitochondrial myopathy and sideroblastic anemia resulting from a missense mutation in the PUS1 gene
  • Investigate chemokine-like factor expression in the idiopathic inflammatory myopathies
  • Demonstrate that fasting activates AceCS2 gene expression by inducing KLF15 transcription factor
  • Characterize human FGFR3-positive sarcoma-initiating stem cells
  • Identify trans-Golgi network proteins and Notch and Hedgehog pathways as putative targets for rhabdomyosarcoma therapy
  • Show that riluzole muscle relaxant effects are mediated by inhibition of INa and stimulation of BKC-channel activity
  • Develop nitric esters that combine the pharmacological functions of NO and muscle relaxation properties for treatment of muscular diseases
  • Develop biodegradable polymer-based transgene delivery vectors for muscular dystrophy treatment
  • Design optimal coating for orthopedic metallic implants
 
 
 
 
 
 
 
 
 

(Click to Enlarge) Human Skeletal Muscle Cells, HSkMC (L). Fused HSkMC (fetal) immunolabeled for desmin (green), nuclei visualized with PI (red) (R).

Details

Tissue
Normal healthy human limb skeletal muscle
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Multinucleated myotubes form after culture in fusion medium
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HSkMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HSkMC (150-05), Growth Medium (151-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Tsfr Med, psg 3, flasks or plates
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HSkMC Normal

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HSkMC Total Kit: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 150K-05f Price: $855.22
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Cryopreserved HSkMC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 150K-05a Price: $855.22
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Cryopreserved HSkMC Total Kit, Pre-Screened: 5x10^5 Cells (Pre-Screened, Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S150K-05f Price: $855.22
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Cryopreserved HSkMC, adult: Frozen HSkMC (5x10^5) Size: 1 Cryovial CAT.#: 150-05a Price: $694.18
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Cryopreserved HSkMC, fetal: Frozen HSkMC (5x10^5) Size: 1 Cryovial CAT.#: 150-05f Price: $694.18
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Cryopreserved HSkMC, fetal, Pre-Screened: Frozen HSkMC (5x10^5) Size: 1 Cryovial CAT.#: S150-05f Price: $785.66
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Cryopreserved HSkMC-COPD Total Kit, Pre-Screened: 5x10^5 Cells (from donor with COPD, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S150COPDK-05a Price: $923.42
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Cryopreserved HSkMC-COPD, adult, Pre-Screened: Frozen HSkMC-COPD from donor with Chronic Obstructive Pulmonary Disease, Adult (5x10^5) Size: 1 Cryovial CAT.#: S150COPD-05a Price: $936.85
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Cryopreserved HSkMC-T2D Total Kit: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 150T2DK-05a Price: $923.42
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Cryopreserved HSkMC-T2D, adult: Frozen HSkMC from donor with Type 2 Diabetes (5x10^5) Size: 1 Cryovial CAT.#: 150T2D-05a Price: $764.14
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Proliferating HSkMC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 151-25a Price: $694.18
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Proliferating HSkMC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 151-75a Price: $898.67
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Proliferating HSkMC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 151-6Wa Price: $898.67
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Proliferating HSkMC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 151-96Wa Price: $1,027.82
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Proliferating HSkMC, fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 151-25f Price: $694.18
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Proliferating HSkMC, fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 151-75f Price: $871.76
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Proliferating HSkMC, fetal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 151-6Wf Price: $871.76
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Proliferating HSkMC, fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 151-96Wf Price: $1,000.91
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: S151-25f Price: $785.66
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: S151-75f Price: $990.15
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: 6 Well CAT.#: S151-6Wf Price: $990.15
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: 96 Well CAT.#: S151-96Wf Price: $1,119.30
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Related Products

Product Size CAT.# Price Quantity
Human Skeletal Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 150-500 Price: $62.42
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Human Skeletal Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 151D-250 Price: $69.96
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Human Skeletal Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 151-500 Price: $118.39
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Human Skeletal Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 151K-500 Price: $128.07
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Human Skeletal Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml (2 parts) CAT.#: 151-GS Price: $65.65
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-IL-6: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.38
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Anti-Myostatin: Mouse Myostatin Antibody Size: 100 ul CAT.#: CP10315 Price: $302.38
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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HSkMC RNA: Total RNA prepared from Human Skeletal Muscle Cells, fetal Size: 10 ug CAT.#: 150-R10f Price: $349.78
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HSkMC RNA: Total RNA prepared from Human Skeletal Muscle Cells, fetal Size: 25 ug CAT.#: 150-R25f Price: $699.56
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HSkMC RNA: Total RNA prepared from Human Skeletal Muscle Cells, Differentiated, fetal Size: 10 ug CAT.#: 150D-R10f Price: $452.03
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HSkMC RNA: Total RNA prepared from Human Skeletal Muscle Cells, Differentiated, fetal Size: 25 ug CAT.#: 150D-R25f Price: $904.05
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HSkMC RNA: Total RNA prepared from Human Skeletal Muscle Cells, adult Size: 10 ug CAT.#: 150-R10a Price: $349.78
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HSkMC RNA: Total RNA prepared from Human Skeletal Muscle Cells, adult Size: 25 ug CAT.#: 150-R25a Price: $699.56
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Human Skeletal Muscle Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 151S-250 Price: $62.42
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Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue Size: 50 ug CAT.#: 1H60-50 Price: $138.57
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Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue Size: 250 ug CAT.#: 1H60-250 Price: $521.98
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IL-6: Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $193.73
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IL-6: Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.31
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IL-6: Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,174.94
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IL-6 ELISA Kit, Human: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $516.60
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.10
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $532.74
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.43
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Myostatin: Human Myostatin Size: 10 ug CAT.#: RP1022-10 Price: $193.73
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Myostatin: Human Myostatin Size: 100 ug CAT.#: RP1022-100 Price: $624.23
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Myostatin: Human Myostatin Size: 1000 ug CAT.#: RP1022-1000 Price: $5,327.44
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Myostatin Propeptide: Human Myostatin Propeptide Size: 25 ug CAT.#: RP1142-25 Price: $193.73
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Myostatin Propeptide: Human Myostatin Propeptide Size: 100 ug CAT.#: RP1142-100 Price: $624.23
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Myostatin Propeptide: Human Myostatin Propeptide Size: 1000 ug CAT.#: RP1142-1000 Price: $5,327.44
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Skeletal Muscle Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2016
Duncan, D., T. Kamerzell and M. Palmer. 2016. Use of Indole Compounds for Fat Reduction and Skin and Soft Tissue Tightening.  Patent Applications US 20160030389 A1.
2014
Iizuka, K., T. Machida, and M. Hirafuji. 2014. Extracellular MCT4 Is a Possible Indicator for Skeletal Muscle MHC Fiber Type Change. Ann Clin Lab Sci, 44:272-276.
2013
Wang, G., and Q. Lu. 2013. A nitrate ester of sedative alkyl alcohol improves muscle function and structure in a murine model of Duchenne muscular dystrophy. Molecular pharmaceutics. 10:3862-3870.
2012
Idris, N., M. Ashraf, R. Ahmed, J. Shujia, and K. Haider. 2012. Activation of IL-11/STAT3 pathway in preconditioned human skeletal myoblasts blocks apoptotic cascade under oxidant stress. Regen Med, 7:47-57.
Nagao, H., T. Setoguchi, S. Kitamoto, Y. Ishidou, S. Nagano, M. Yokouchi, M. Abematsu, N. Kawabata, S. Maeda, S. Yonezawa, and S. Komiya. 2012. RBPJ Is a Novel Target for Rhabdomyosarcoma Therapy. PloS one. 7:e39268.
Wang, M., J.D. Tucker, P. Lu, B. Wu, C. Cloer, and Q. Lu. 2012. Tris[2-(acryloyloxy)ethyl]isocyanurate Cross-Linked Low-Molecular-Weight Polyethylenimine as Gene Delivery Carriers in Cell Culture and Dystrophic mdx Mice. Bioconjugate chemistry. 23:837-845.
2011
Kawabata, N., K. Ijiri, Y. Ishidou, T. Yamamoto, H. Nagao, S. Nagano, S. Maeda, S. Komiya, and T. Setoguchi. 2011. Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth. International journal of oncology. 39:899.
Kunigou, O., H. Nagao, N. Kawabata, Y. Ishidou, S. Nagano, S. Maeda, S. Komiya, and T. Setoguchi. 2011. Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma. Oncology reports. 26:1337-1342.
2010
Thuangtong, R., J.J. Bentow, K. Knopp, N.A. Mahmood, N.E. David, and M.S. Kolodney. 2010. TissueSelective Effects of Injected Deoxycholate. Dermatologic Surgery. 36:899-908.
Zalevsky, J., D. Nguyen, G. Moore, S. Ezhevsky, J. Desjarlais, A. Chirino, D. Cash, and M. Bernett. 2010. Pharmaceutical compositions of adiponectin variants and methods of storage. Patent US 7678886 B2.
Zalevsky, J., D. Nguyen, G. Moore, S. Ezhevsky, J. Desjarlais, A. Chirino, D. Cash, and M. Bernett. 2010. Adiponectin variants. Patent US 7709607 B2.
Zalevsky, J., D. Nguyen, G. Moore, S. Ezhevsky, J. Desjarlais, A. Chirino, D. Cash, and M. Bernett. 2010. Method of treatment using adiponectin variants. Patent US 7749956 B2.
2009
Gupta, A., C. Lobocki, S. Singh, M. Robertson, O.A. Akadiri, G. Malhotra, and I.T. Jackson. 2009. Actions and Comparative Efficacy of Phosphatidylcholine Formulation and Isolated Sodium Deoxycholate for Different Cell Types. Aesth Plast Surg. 33:346-352.
Hirotsu, M., T. Setoguchi, Y. Matsunoshita, H. Sasaki, H. Nagao, H. Gao, K. Sugimura, and S. Komiya. 2009. Tumour formation by single fibroblast growth factor receptor 3-positive rhabdomyosarcoma-initiating cells. Br J Cancer. 101:2030-2037.
Peters, L., and T. Lorenz. 2009. Postsynaptically Targeted Chemodenervation Agents and Their Methods of Use. Patent Application US 20110118190 A1.
Xu, J., S. Ekins, M. McGlashen, and D. Lauffenburger. 2009. FUTURE PERSPECTIVES OF BIOLOGICAL ENGINEERING IN PHARMACEUTICAL RESEARCH: THE PARADIGM OF MODELING, MINING, MANIPULATION, AND MEASUREMENTS. Ch. 14 inXu, J. and Ekins, S. 2009. Drug Efficacy, Safety, and Biologics Discovery: Emerging Technologies and Tools.
Xu, J. and L. Yu. 2009. Focus on the Fundamentals: Toward Better Therapeutic Index Prediction. Pg 19.  In: Drug Efficacy, Safety, and Biologics Discovery: Emerging Technologies and Tools.  Ekins S. and X Jinghai, Eds. John Wiley & Sons, Inc.
Zalevsky, J., D. Nguyen, G. Moore, S. Ezhevsky, J. Desjarlais, A. Chirino, D. Cash, and M. Bernett. 2009. Globular adiponectin variants. Patent US 7592423 B2.
2008
Wang, Y.-J., M.-W. Lin, A.-A. Lin, and S.-N. Wu. 2008. Riluzole-induced block of voltage-gated Na+ current and activation of BKCa channels in cultured differentiated human skeletal muscle cells. Life sciences. 82:11-20.
2006
Cash, D., A. Chirino, J. Desjarlais, S. Ezhevsky, G. Moore, D. Nguyen, and J. Zalevsky. 2006. Adiponectin variants. Patent Application US 20070015909 A1.
Perla, V., and T.J. Webster. 2006. nano-hydroxyapatite–thermally denatured small intestine sub-mucosa composites for entheses applications. International Journal of nanomedicine. 1:351.
Yamazaki, H., M. Suzuki, T. Aoki, S. Morikawa, T. Maejima, F. Sato, K. Sawanobori, M. Kitahara, T. Kodama, and Y. Saito. 2006. Influence of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on ubiquinone levels in rat skeletal muscle and heart: relationship to cytotoxicity and inhibitory activity for cholesterol synthesis in human skeletal muscle cells. Journal of atherosclerosis and thrombosis. 13:295-307.
Zalevsky, J., D. Nguyen, G. Moore, S. Ezhevsky, J. Desjarlais, A. Chirino, D. Cash, and M. Bernett. 2006. Rational Chemical Modification of Adiponectin Variants. Patent Application US 20070054359 A1.
2005
Morikawa, S., T. Murakami, H. Yamazaki, A. Izumi, Y. Saito, T. Hamakubo, and T. Kodama. 2005. Analysis of the global RNA expression profiles of skeletal muscle cells treated with statins. J. atherosclerosis & thrombosis. 12:121-131.
Patton, J.R., Y. Bykhovskaya, E. Mengesha, C. Bertolotto, and N. Fischel-Ghodsian. 2005. Mitochondrial Myopathy and Sideroblastic Anemia (MLASA): Missense mutation in the pseudouridine synthase 1 (PUS1) gene is associated with the loss of tRNA pseudouridylation. J. Biological Chemistry. 280:19823-19828.
Perla, V., M. Sato, and T.J. Webster. 2005. Increased Skeletal Muscle Cell and Osteoblast Numbers on Hydrothermally-Treated Nano-Hydroxyapatite/Collagen Type I Composites for Entheses Applications. Journal of Biomedical Nanotechnology. 1:297-305.
2004
Yamamoto, J., Y. Ikeda, H. Iguchi, T. Fujino, T. Tanaka, H. Asaba, S. Iwasaki, R.X. Ioka, I.W. Kaneko, K. Magoori, S. Takahashi, T. Mori, H. Sakaue, T. Kodama, M. Yanagisawa, T.T. Yamamoto, S. Ito, and J. Sakai. 2004. A Krüppel-like factor KLF15 Contributes Fasting-induced Transcriptional Activation of Mitochondrial Acetyl-CoA Synthetase Gene AceCS2. Journal of Biological Chemistry. 279:16954-16962.