MSDS Cryopreserved Cells
Instructions HSkMC
Cell Apps Flyer Skeletal Muscle Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Pre-Screened for Insulin & AMPK signaling are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle.  Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK & Insulin Signaling Pathways.
AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise.  AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.
Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action.  Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell (see Fig.B).
(Click to Enlarge) Human Skeletal Muscle Cells (HSkMC) response to forced activation of AMPK, leading to ACC phosphorylation.  Cells serum-starved 16 hr & treated for 0, 15 or 30 min w/ 100nM oligomycin, which inhibits oxidative phosphorylation & causes activation of AMPK (A). Simplified insulin signal transduction map.  Specific phosphorylation events examined in Fig. C are marked in red (B).  HSkMC response to insulin signaling leading to phosphorylation of IRS-1, Akt, GSK-3b, MAPK/ERK and p70S6K.  Cells serum-starved 16 hr & stimulated w/ 100nM insulin for 0, 15 or 30 min.

Details

Tissue
Normal healthy human limb skeletal muscle
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
500,000 HSkMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HSkMC (150-05f), Growth Medium (151-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HSkMC

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Instructions HSkMC

Format: PDF

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Instructions HSkMC

Format: PDF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HSkMC Total Kit, Pre-Screened: 5x10^5 Cells (Pre-Screened, Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S150K-05f Price: $855.22
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Cryopreserved HSkMC, fetal, Pre-Screened: Frozen HSkMC (5x10^5) Size: 1 Cryovial CAT.#: S150-05f Price: $785.66
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Cryopreserved HSkMC-COPD Total Kit, Pre-Screened: 5x10^5 Cells (from donor with COPD, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S150COPDK-05a Price: $923.42
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Cryopreserved HSkMC-COPD, adult, Pre-Screened: Frozen HSkMC-COPD from donor with Chronic Obstructive Pulmonary Disease, Adult (5x10^5) Size: 1 Cryovial CAT.#: S150COPD-05a Price: $936.85
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: S151-25f Price: $785.66
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: S151-75f Price: $990.15
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: 6 Well CAT.#: S151-6Wf Price: $990.15
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Proliferating HSkMC, fetal, Pre-Screened: Actively growing, dividing cells in medium Size: 96 Well CAT.#: S151-96Wf Price: $1,119.30
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Related Products

Product Size CAT.# Price Quantity
Human Skeletal Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 150-500 Price: $62.42
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Human Skeletal Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 151D-250 Price: $69.96
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Human Skeletal Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 151-500 Price: $118.39
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Human Skeletal Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 151K-500 Price: $128.07
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Human Skeletal Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml (2 parts) CAT.#: 151-GS Price: $65.65
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-IL-6: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.38
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Anti-Myostatin: Mouse Myostatin Antibody Size: 100 ul CAT.#: CP10315 Price: $302.38
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Human Skeletal Muscle Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 151S-250 Price: $62.42
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IL-6: Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $193.73
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IL-6: Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.31
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IL-6: Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,174.94
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IL-6 ELISA Kit, Human: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $516.60
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.10
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $532.74
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IL-6, Animal-Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.43
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Myostatin: Human Myostatin Size: 10 ug CAT.#: RP1022-10 Price: $193.73
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Myostatin: Human Myostatin Size: 100 ug CAT.#: RP1022-100 Price: $624.23
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Myostatin: Human Myostatin Size: 1000 ug CAT.#: RP1022-1000 Price: $5,327.44
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Myostatin Propeptide: Human Myostatin Propeptide Size: 25 ug CAT.#: RP1142-25 Price: $193.73
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Myostatin Propeptide: Human Myostatin Propeptide Size: 100 ug CAT.#: RP1142-100 Price: $624.23
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Myostatin Propeptide: Human Myostatin Propeptide Size: 1000 ug CAT.#: RP1142-1000 Price: $5,327.44
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

Cell Apps Flyer Skeletal Muscle Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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