Instructions HScAEC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Subclavian Artery Endothelial Cells (HScAEC), along with human aortic (HAOEC), carotid artery (HCtAEC), coronary artery (HCAEC) and brachiocephalic artery (HBcAEC), all from Cell Applications, Inc., have been used to:
  • Demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response
  • The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions
  • This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli.  Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.  To eliminate the donor-to-donor variability, the scientists took advantage of the great variety of primary cells offered by Cell Applications, including the option of ordering a panel of endothelial cells obtained from different vascular beds of the same donor!
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
(Click to Enlarge) Human Subclavian Artery Endothelial Cells: HScAEC (L).  HScAEC immunolabeled with Anti-CD31 antibodies (green); nuclei are counterstained with PI (red) (R).

Details

Tissue
Normal healthy human subclavian artery
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag, DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HScAEC frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HScAEC (3010-05a), Growth Medium (212-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Growth Med (flasks or plates)
DoublingsAt least 5
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HScAEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HScAEC, Adult: Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 3010K-05a Price: $911.00
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Cryopreserved HScAEC, Adult: Frozen HScAEC (5x10^5) Size: 1 Cryovial CAT.#: 3010-05a Price: $748.00
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Proliferating HScAEC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 3011-25a Price: $748.00
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Proliferating HScAEC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 3011-75a Price: $924.00
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Proliferating HScAEC, Adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 3011-24Wa Price: $911.00
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Proliferating HScAEC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 3011-96Wa Price: $1,040.00
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Related Products

Product Size CAT.# Price Quantity
Human Meso Endo Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 212-500 Price: $118.00
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Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 212K-500 Price: $128.00
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Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red Size: yields 500 ml CAT.#: 212KPR-500 Price: $130.00
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Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 212A-500 Price: $124.00
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Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS Size: 500 ml CAT.#: 212F-500 Price: $136.00
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Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red Size: 500 ml CAT.#: 212PR-500 Price: $118.00
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Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 212-GS Price: $62.00
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Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS Size: 5 ml CAT.#: 212F-GS Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-CD31: Mouse CD31 Antibody Size: 100 ul CAT.#: CC10038 Price: $325.00
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HSCAEC RNA, Adult: Total RNA prepared from Human Subclavian Artery Endothelial Cells, adult Size: 10 ug CAT.#: 3010-R10a Price: $409.00
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HSCAEC RNA, Adult: Total RNA prepared from Human Subclavian Artery Endothelial Cells, adult Size: 25 ug CAT.#: 3010-R25a Price: $818.00
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Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $587.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $139.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $522.00
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Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.00
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Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $517.00
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Human PECAM-1/CD31 ELISA Kit: Human Platelet Endothelial Cell Adhesion Molecule-1/Cluster of Differentiation 31 ELISA Kit Size: 96 Wells CAT.#: CL0883 Price: $587.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $194.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,090.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $533.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,499.00
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Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.00
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Monoclonal CD31 Antibody: Monoclonal CD31 Antibody Size: 100 ul CAT.#: CB13678 Price: $302.00
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Mouse PECAM-1/CD31 ELISA Kit: Mouse Platelet Endothelial Cell Adhesion Molecule-1/Cluster of Differentiation 31 Size: 96 Wells CAT.#: CL0884 Price: $533.00
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Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody Size: 100 ul CAT.#: CA1080 Price: $302.00
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Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.00
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Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2013
Scott, D.W., M.O. Vallejo, and R.P. Patel. 2013. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. Journal of the American Heart Association. 2:e000263-e000263.