Instructions HTMC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description

 

Human Trabecular Meshwork Cells (HTMC) are endothelial-like cells in a sponge-like connective tissue located near the front of the eye.  HTMC make layers of beams, part of a fibrous basement membrane containing extracellular matrix and cells.  In this area of high outflow resistance, HTMC regulate eye pressure by controlling drainage of fluid into tubes that flow into the bloodstream.  Outflow is mediated by alterations in contractility and tension of HTMC, which also serve as a self-cleaning filter due to their phagocytic nature.  Live cell imaging of the cytoskeleton provides valuable information on actin dynamics in HTMC.  Other research aims to identify treatments that relax HTMC contraction to increase fluid outflow and lower eye pressure.  Specific targeting of HTMC could also play a clinical role by increasing therapeutic efficacy of nanoparticles for gene delivery.
Damage and dysfunction of HTMC have clinical significance.  For instance, hypoxia increases DNA methylation, accompanied by altered gene expression.  During the normal aging process, HTMC number decreases, and senescent cells accumulate.  In glaucoma, a leading cause of irreversible blindness, decreased fluid outflow causes an elevation of intraocular pressure and progressive loss of retinal ganglion cells.  Physical changes to the TM include increased fibrosis, fibronectin accumulation, and expression of ECM cross-linking enzymes.  This cytoskeletal reorganization and cell loss causes the TM to become rigid and stiff.  Other glaucoma-related dysfunctions include mitochondrial defects, altered signaling pathways, elevated TGF-β2, genomic DNA defects, and oxidation damage.
(Click to enlarge) Human Trabecular Meshwork Cells: HTMC (Left)
Dexamethasone treatment in HTMC induced actin network rearrangement (arrowheads in B), upregulation of fibronectin (D) and α-smooth muscle actin (F) expression compared to untreated control (A, C, E).

Details

TissueNormal healthy human adult eyes
QCNo bacteria, yeast, fungi, mycoplasma, virus
CharacterPositive for Fibronectin
BioassayAttach, spread, proliferate in Growth Med
Cryovial~500,000 HTMC (1st passage) in Freezing Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen HTMC, Growth Medium (631-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in HTMC Growth Medium, psg 2, flasks or plates
DoublingsAt least 12
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HTMC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HTMC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 634K-05a Price: $864.76
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Cryopreserved HTMC, adult: Frozen HTMC (5x10^5) Size: 1 Cryovial CAT.#: 634-05a Price: $686.75
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Proliferating HTMC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 635-25a Price: $686.75
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Proliferating HTMC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 635-75a Price: $896.88
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Proliferating HTMC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 635-6Wa Price: $896.88
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Proliferating HTMC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 635-96Wa Price: $1,019.88
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Related Products

Product Size CAT.# Price Quantity
Human Trabecular Meshwork Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: 630-500 Price: $55.35
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Human Trabecular Meshwork Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 631-500 Price: $104.55
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Human Trabecular Meshwork Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 631K-500 Price: $112.75
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Human Trabecular Meshwork Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 631-GS Price: $58.43
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Fibronectin ELISA Kit, Human: Human Fibronectin ELISA Kit Size: 96 Wells CAT.#: CL0349 Price: $457.41
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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