Instructions HTEpC Normal
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Tracheal Epithelial Cells (HTEpC) provide a useful model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, pulmonary diseases and smoking.  Note that Cell Applications, Inc. offers HTEpC obtained from healthy donors, as well as from asthma patients.
HTEpC from Cell Applications, Inc. have been used in a study investigating the role of goblet cell hyperplasia, possibly mediated by the expression of SPDEF, to the pathogenesis of chronic pulmonary diseases.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
(Click to Enlarge) Top: Human Tracheal Epithelial Cells: HTEpC (L). HTEpC immunolabeled with an anti-CK18 antibody (R). Bottom: HTEpC (L) transfected with GFP plasmid DNA using the Cytofect™ Epithelial Cell Transfection Kit (R).

Details

Tissue
Surface epithelium of normal healthy human trachea
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Medium
Cryovial
500,000 HTEpC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HTEpC (504-05a), Growth Medium (511-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HTEpC Normal

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HTEpC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 504K-05a Price: $866.00
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Cryopreserved HTEpC, Adult: Frozen HTEpC (5x10^5) Size: 1 Cryovial CAT.#: 504-05a Price: $695.00
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Cryopreserved HTEpC-AS, Adult: Frozen HTEpC-AS from donor with Asthma Size: 1 Cryovial CAT.#: 504AS-05a Price: $765.00
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Cryopreserved HTEpC-AS, Adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 504ASK-05a Price: $931.00
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Proliferating HTEpC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 505-25a Price: $695.00
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Proliferating HTEpC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 505-75a Price: $900.00
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Proliferating HTEpC, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 505-6Wa Price: $900.00
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Proliferating HTEpC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 505-96Wa Price: $1,029.00
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Related Products

Product Size CAT.# Price Quantity
HBrEpC/HTrEpC Basal Medium: Without growth supplement (GS).  Add GS before use. Size: 500 ml CAT.#: 510-500 Price: $62.00
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HBrEpC/HTrEpC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 511D-250 Price: $129.00
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HBrEpC/HTrEpC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 511-500 Price: $126.00
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HBrEpC/HTrEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Kit CAT.#: 511K-500 Price: $125.00
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HBrEpC/HTrEpC Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 511A-500 Price: $125.00
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HBrEpC/HTrEpC Growth Medium wo BPE: Growth medium without BPE Size: 500 ml CAT.#: 511B-500 Price: $125.00
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HBrEpC/HTrEpC Growth Medium wo BPE, Hydrocortisone, Insulin: Growth medium without BPE, hydrocortisone and insulin Size: 500 ml CAT.#: 511BHI-500 Price: $125.00
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HBrEpC/HTrEpC Growth Medium wo T3: Growth medium without Tri-iodothyronine (T3) Size: 500 ml CAT.#: 511T3-500 Price: $125.00
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HBrEpC/HTrEpC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 511-GS Price: $62.00
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Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 140-500 Price: $74.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $404.00
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HTEpC RNA, Adult: Total RNA prepared from Human Tracheal Epithelial Cells, adult Size: 10 ug CAT.#: 504-R10a Price: $350.00
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HTEpC RNA, Adult: Total RNA prepared from Human Tracheal Epithelial Cells, adult Size: 25 ug CAT.#: 504-R25a Price: $700.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 1000 ug CAT.#: RP1026AF-1000 Price: $290.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 100 ug CAT.#: RP1026AF-100 Price: $95.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 500 ug CAT.#: RP1026AF-500 Price: $213.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 100 ug CAT.#: RP1026-100 Price: $86.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 500 ug CAT.#: RP1026-500 Price: $194.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 1000 ug CAT.#: RP1026-1000 Price: $264.00
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Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit Size: 96 wells CAT.#: CL0389 Price: $484.00
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Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit Size: 96 wells CAT.#: CL0392 Price: $587.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 10 ug CAT.#: RP1173-10 Price: $194.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 100 ug CAT.#: RP1173-100 Price: $624.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 1000 ug CAT.#: RP1173-1000 Price: $5,327.00
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Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody Size: 100 ul CAT.#: CB19846 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Epithelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Chakraborty, S., V. Castranova, M. Perez and G. Piedimonte. 2017. Nanoparticles-induced apoptosis of human airway epithelium is mediated by proNGF/p75NTR signalingJ Toxicol & Env Hlth, Pt A, 80:53-68.
2016
Liu, Z., X. Chen, Q. Wu, J. Song, L. Wang and G. Li. 2016.miR-125b inhibits goblet cell differentiation in allergic airway inflammation by targeting SPDEF. Eur J Pharmacol, 782:14-20.
2007
Park, K.-S., T.R. Korfhagen, M.D. Bruno, J.A. Kitzmiller, H. Wan, S.E. Wert, G.K. Khurana Hershey, G. Chen, and J.A. Whitsett. 2007. SPDEF regulates goblet cell hyperplasia in the airway epithelium. J. clinical investigation. 117:978-988.
2006
Whitsett, J., and J. Wells. 2006. Regulatory proteins in lung repair and treatment of lung disease. Patent Application US 20110177996 A1.