Instructions HUAEC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Umbilical Artery Endothelial Cells (HUAEC) provided by Cell Applications, Inc. have been used to investigate the influence of soluble factors secreted by tumor cells on the expression of cell surface antigens by endothelial cells (Moehler, 2008).  It was determined that tumor-derived factors activated transcription of FUT1 which lead to enhanced expression of CD174, a molecule involved in cell-cell adhesion and migration during tumor angiogenesis.

(Click to Enlarge) Human Umbilical Artery Endothelial Cells, HUAEC (L). HUAEC immunolabeled with anti-vWF antibodies (green); nuclei are stained with PI (red) (R).

Details

Tissue
Normal healthy human umbilical arteries
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag, DiI-Ac-LDL 
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HUAEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HUAEC, Growth Medium (212-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HUAEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HUAEC, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 202K-05n Price: $575.00
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Cryopreserved HUAEC, Neonatal: Frozen HUAEC (5x10^5) Size: 1 Cryovial CAT.#: 202-05n Price: $412.00
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Proliferating HUAEC, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 203-25n Price: $412.00
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Proliferating HUAEC, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 203-75n Price: $617.00
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Proliferating HUAEC, Neonatal: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 203-24Wn Price: $617.00
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Proliferating HUAEC, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 203-96Wn Price: $746.00
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Related Products

Product Size CAT.# Price Quantity
Human Meso Endo Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 212-500 Price: $118.00
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Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 212K-500 Price: $128.00
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Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red Size: yields 500 ml CAT.#: 212KPR-500 Price: $130.00
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Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 212A-500 Price: $124.00
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Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS Size: 500 ml CAT.#: 212F-500 Price: $136.00
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Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red Size: 500 ml CAT.#: 212PR-500 Price: $118.00
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Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 212-GS Price: $62.00
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Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS Size: 5 ml CAT.#: 212F-GS Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HUAEC RNA, Neonatal: Total RNA prepared from Human Umbilical Artery Endothelial Cells, neonatal Size: 10 ug CAT.#: 202-R10n Price: $350.00
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HUAEC RNA, Neonatal: Total RNA prepared from Human Umbilical Artery Endothelial Cells, neonatal Size: 25 ug CAT.#: 202-R25n Price: $700.00
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Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $587.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.00
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Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $517.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $194.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.00
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Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,090.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $533.00
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Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,499.00
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Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.00
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Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.00
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Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2015
Bowers, S., Meng, C., Davis, M., and Davis, G. 2015. Investigating Human Vascular Tube Morphogenesis and Maturation Using Endothelial Cell-Pericyte Co-cultures and a Doxycycline-Inducible Genetic System in 3D Extracellular Matrices.  Methods in Molecular Biology, 1189:171-189.
2008
Moehler, T.M., S. Sauer, M. Witzel, M. Andrulis, J.J. GarciaVallejo, R. Grobholz, M. WillhauckFleckenstein, A. Greiner, H. Goldschmidt, and R. SchwartzAlbiez. 2008. Involvement of α 12fucosyltransferase I (FUT1) and surfaceexpressed lewisy (CD174) in first endothelial cell–cell contacts during angiogenesis. J. Cell. Phys. 215:27-36.