Instructions HUASMC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Umbilical Artery Smooth Muscle Cells (HUASMC) from Cell Applications, Inc. have been used to study:
- Effects of snake venom on smooth muscle cells
- Secretion mechanism of the (pro) renin receptor
- The role of proteasomes in the development of cardiovascular diseases
- Novel material surfaces to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Umbilical Artery Smooth Muscle Cells: HUASMC. Isolated from a paired artery found in the abdominal and pelvic regions, and which in the fetus, extends into the umbilical cord.
Details
Tissue | Normal healthy human umbilical artery |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HUASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HUASMC (252-05n), Growth Medium (311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved HUASMC, Neonatal: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 252K-05n | Price: $568.00 | |
| Cryopreserved HUASMC, Neonatal: Frozen HUASMC (5x10^5) | Size: 1 Cryovial | CAT.#: 252-05n | Price: $414.00 | |
| Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 253-25n | Price: $414.00 | |
| Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 253-75n | Price: $619.00 | |
| Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium | Size: 6 Well | CAT.#: 253-6Wn | Price: $619.00 | |
| Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 253-96Wn | Price: $748.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 310-500 | Price: $57.00 | |
| Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 311D-250 | Price: $73.00 | |
| Human SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 311-500 | Price: $105.00 | |
| Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 311K-500 | Price: $113.00 | |
| Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 311-GS | Price: $57.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $55.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $139.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $36.00 | |
| Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $285.00 | |
| Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $54.00 | |
| HUASMC RNA, Neonatal: Total RNA prepared from Human Umbilical Smooth Muscle Cells, neonatal | Size: 10 ug | CAT.#: 252-R10n | Price: $350.00 | |
| HUASMC RNA, Neonatal: Total RNA prepared from Human Umbilical Smooth Muscle Cells, neonatal | Size: 25 ug | CAT.#: 252-R25n | Price: $700.00 | |
| Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit | Size: 96 Wells | CAT.#: CL0410 | Price: $517.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 20 ug | CAT.#: RP1010AF-20 | Price: $213.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 100 ug | CAT.#: RP1010AF-100 | Price: $533.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 1000 ug | CAT.#: RP1010AF-1000 | Price: $3,492.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 20 ug | CAT.#: RP1010-20 | Price: $194.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 100 ug | CAT.#: RP1010-100 | Price: $484.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 1000 ug | CAT.#: RP1010-1000 | Price: $3,175.00 | |
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | Size: 100 ul | CAT.#: CP10325 | Price: $302.00 |
Resources/Documents
Publications
2016
Cooper, K., I. Chun, D. Colter, S. Dhanaraj, A. Gosiewska, A. Seyda, C. Fang and C. Yang. 2016. Tissue engineered blood vessel. Patent US 9290742 B2.
2014
Martin, D., X. Wang, Y. Li, H. Ford, B. Kjellsen, and S. Li. 2014. Angiotensin II activates the proteasome and stimulates vascular smooth muscle cell proliferation (866.10). FASEB J. 28:866.810.
2012
Kanie, K., Y. Narita, Y. Zhao, F. Kuwabara, M. Satake, S. Honda, H. Kaneko, T. Yoshioka, M. Okochi, H. Honda, and R. Kato. 2012. Collagen type IV-specific tripeptides for selective adhesion of endothelial and smooth muscle cells. Biotech. & Bioeng. 109:1808-1816.
2011
Yoshikawa, A., Y. Aizaki, K.-i. Kusano, F. Kishi, T. Susumu, S. Iida, S. Ishiura, S. Nishimura, M. Shichiri, and T. Senbonmatsu. 2011. The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space. Hypertension research. 34:599-605.
2008
Mora, J., R. Mora, B. Lomonte, and J.M. Gutiérrez. 2008. Effects of Bothrops asper Snake Venom on Lymphatic Vessels: Insights into a Hidden Aspect of Envenomation. PLoS Negl Trop Dis. 2:e318.