Instructions HUASMC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Umbilical Artery Smooth Muscle Cells (HUASMC) from Cell Applications, Inc. have been used to study:
  • Effects of snake venom on smooth muscle cells
  • Secretion mechanism of the (pro) renin receptor
  • The role of proteasomes in the development of cardiovascular diseases
  • Novel material surfaces to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Umbilical Artery Smooth Muscle Cells: HUASMC. Isolated from a paired artery found in the abdominal and pelvic regions, and which in the fetus, extends into the umbilical cord.

Details

Tissue
Normal healthy human umbilical artery
QC
No bacteria, yeast, fungi, mycoplasma, virus 
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HUASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HUASMC (252-05n), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HUASMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HUASMC, Neonatal: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 252K-05n Price: $564.00
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Cryopreserved HUASMC, Neonatal: Frozen HUASMC (5x10^5) Size: 1 Cryovial CAT.#: 252-05n Price: $414.00
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Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 253-75n Price: $619.00
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Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 253-24Wn Price: $619.00
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Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 253-96Wn Price: $748.00
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Proliferating HUASMC, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 253-25n Price: $414.00
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Related Products

Product Size CAT.# Price Quantity
Human SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.00
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Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.00
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Human SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.00
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Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.00
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Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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HUASMC RNA, Neonatal: Total RNA prepared from Human Umbilical Smooth Muscle Cells, neonatal Size: 10 ug CAT.#: 252-R10n Price: $350.00
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HUASMC RNA, Neonatal: Total RNA prepared from Human Umbilical Smooth Muscle Cells, neonatal Size: 25 ug CAT.#: 252-R25n Price: $700.00
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Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $517.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $533.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,175.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $194.00
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Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2016
Cooper, K., I. Chun, D. Colter, S. Dhanaraj, A. Gosiewska, A. Seyda, C. Fang and C. Yang. 2016. Tissue engineered blood vessel. Patent US 9290742 B2.
2014
Martin, D., X. Wang, Y. Li, H. Ford, B. Kjellsen, and S. Li. 2014. Angiotensin II activates the proteasome and stimulates vascular smooth muscle cell proliferation (866.10). FASEB J. 28:866.810.
2012
Kanie, K., Y. Narita, Y. Zhao, F. Kuwabara, M. Satake, S. Honda, H. Kaneko, T. Yoshioka, M. Okochi, H. Honda, and R. Kato. 2012. Collagen type IV-specific tripeptides for selective adhesion of endothelial and smooth muscle cells. Biotech. & Bioeng. 109:1808-1816.
2011
Yoshikawa, A., Y. Aizaki, K.-i. Kusano, F. Kishi, T. Susumu, S. Iida, S. Ishiura, S. Nishimura, M. Shichiri, and T. Senbonmatsu. 2011. The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space. Hypertension research. 34:599-605.
2008
Mora, J., R. Mora, B. Lomonte, and J.M. Gutiérrez. 2008. Effects of Bothrops asper Snake Venom on Lymphatic Vessels: Insights into a Hidden Aspect of Envenomation. PLoS Negl Trop Dis. 2:e318.