Instructions HUVSMC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Umbilical Vein Smooth Muscle Cells (HUVSMC) from Cell Applications, Inc. have been used in studies evaluating treatments that involve blood vessel stents. 
  • Specifically, intra-shunt delivery of motexafin gadolinium was shown to inhibit the proliferation of SMCs during treatment of portal hypertension
  • Also, high-density lipoproteins (HDL) exhibit cardioprotective and anti-inflammatory properties by reducing HUVSMC expression of chemokines CCL2, CCL5, and CX3CL1, and chemokine receptors CCR2 and CX3CR1 (via NF-κB/pAkt inhibition) and by decreasing cell proliferation (via pERK inhibition), highlighting HDL importance in preventing neointimal hyperplasia, the main cause of early vein graft/stent failure
  • HUVSMC were also used to demonstrate that genes coding for muscle and non-muscle actin proteins are transcribed by different transcription machinery
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Umbilical Vein Smooth Muscle Cells: HUVSMC.  Cells isolated from the vessel that carries oxygenated blood from the placenta to the fetus.

Details

Tissue
Normal healthy human umbilical vein
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HUVSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HUVSMC (250-05n), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 6
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HUVSMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HUVSMC, Neonatal: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 250K-05n Price: $683.00
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Cryopreserved HUVSMC, Neonatal: Frozen HUVSMC (5x10^5) Size: 1 Cryovial CAT.#: 250-05n Price: $533.00
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Proliferating HUVSMC, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 251-96Wn Price: $866.00
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Proliferating HUVSMC, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 251-25n Price: $533.00
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Proliferating HUVSMC, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 251-75n Price: $737.00
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Proliferating HUVSMC, Neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 251-6Wn Price: $737.00
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Related Products

Product Size CAT.# Price Quantity
Human SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.00
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Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.00
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Human SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.00
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Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.00
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Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $517.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $533.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,175.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $194.00
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HUVSMC RNA, Neonatal: Total RNA prepared from Human Umbilical Vein Smooth Muscle Cells, neonatal Size: 10 ug CAT.#: 250-R10n Price: $409.00
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HUVSMC RNA, Neonatal: Total RNA prepared from Human Umbilical Vein Smooth Muscle Cells, neonatal Size: 25 ug CAT.#: 250-R25n Price: $818.00
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Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017

Vanags, L.Z., Tan, J.T., Santos, M., Michael, P.S., Ali, Z., Bilek, M.M., Wise, S.G. and Bursill, C.A., 2017. Plasma activated coating immobilizes apolipoprotein AI to stainless steel surfaces in its bioactive form and enhances biocompatibility. Nanomedicine: Nanotechnology, Biology and Medicine. DOI: 10.1016/j.nano.2017.06.012

2015
Gaston, B., A. Straub, B. Isakson, and L. Columbus. 2015. Compositions and Methods for Regulating Arterial Tone.  Patent Application US 20150274779 A1.
2014
Thakali, K., J. Saben, J. Faske, F. Lindsey, H. Gomez-Acevedo, C. Lowery, T. Badger A. Andres and K. Shankar. 2014. Maternal pregravid obesity changes gene expression profiles toward greater inflammation and reduced insulin sensitivity in umbilical cordPediatric Res, 76:202-210.
2013
van der Vorst, E.P.C., L.Z. Vanags, L.L. Dunn, H.C. Prosser, K.-A. Rye, and C.A. Bursill. 2013. High-density lipoproteins suppress chemokine expression and proliferation in human vascular smooth muscle cells. The FASEB Journal. 27:1413-1425.
Wang, H., F. Zhang, Y. Meng, T. Zhang, P. Willis, T. Le, S. Soriano, E. Ray, K. Valji, G. Zhang, and X. Yang. 2013. MRI-Monitored Intra-Shunt Local Agent Delivery of Motexafin Gadolinium: Towards Improving Long-Term Patency of TIPS. PloS one. 8:e57419.
2011
Lee, Y.-L., C.-H. Chiao, and M.-T. Hsu. 2011. Transcription of Muscle Actin Genes by a Nuclear Form of Mitochondrial RNA Polymerase. PloS one. 6:e22583.
2010
Gelissen, I.C., S. Cartland, A.J. Brown, C. Sandoval, M. Kim, D.L. Dinnes, Y. Lee, V. Hsieh, K. Gaus, L. Kritharides, and W. Jessup. 2010. Expression and stability of two isoforms of ABCG1 in human vascular cells. Atherosclerosis. 208:75-82.
Maier, K., X. Han, B. Sadowitz, K. Gentile, F. Middleton and V. Gahtan. 2010. Thrombospondin-1: A proatherosclerotic protein augmented by hyperglycemia. J Vasc Surg, 51:1238-1247.
2007
Du, W., K.M. Pryse, J.A. Fee, E.L. Elson, and R.J. Okamoto. 2007. Vascular Smooth Muscle Cell Mechanics During Cyclic Stretch: Effect of Serum and a Serum Substitute. In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers. 267-268.