IGF-I: Rat Insulin-like Growth Factor-I

Product Sheet RP3001

Description

BACKGROUND Insulin-like Growth Factor I, IGF-I, is a growth factor produced in response to growth hormone-stimulated liver and can be found circulating throughout the body and throughout life. IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100% homologous with bovine and porcine proteins.

Recombinant rat IGF-I is a non-glycosylated protein, containing 70 amino acids and having a molecular mass of 7.7 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP3001
 
Alternative Name:
Somatamedin C, mechano growth factor, IGF-IA
 
Source:
E. coli
 
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
 
Formulation:
Recombinant rat IGF-I is lyophilized with no additives.
 
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
 
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
 
Biological Activity:
The activity is measured in a serumfree cell proliferation assay using MCF7 cells and is typically 0.6-3 ng/mL.
 
AA Sequence:
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
 

Products

Product Size CAT.# Price Quantity
Rat Insulin-Like Growth Factor I (IGF-1): Rat Insulin-like Growth Factor-I Size: 10 ug CAT.#: RP3001-10 Price: $86.00
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Rat Insulin-Like Growth Factor I (IGF-1): Rat Insulin-like Growth Factor-I Size: 50 ug CAT.#: RP3001-50 Price: $194.00
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Rat Insulin-Like Growth Factor I (IGF-1): Rat Insulin-like Growth Factor-I Size: 1000 ug CAT.#: RP3001-1000 Price: $1,399.00
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Resources/Documents

Product Sheet RP3001