Product Sheet RP2025
Description
BACKGROUND Interleukin 12 (IL-12) is an inducer of cell-mediated immunity and is produced by dendritic cells, macrophages, and B cells after antigen stimulation. IL-12 functions to promote naïve T cell differentiation into the type 1 T helper cell (Th1) lineage. IL-12 also stimulates the production of interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) from T cells and natural killer cells. Mouse IL-12 is active on human and mouse cells.
Recombinant mouse interleukin-12 is a non-glycosylated 507 aa protein consisting of p35: 194 aa and p40: 313. The total molecular mass of 57.6 kDa includes p35: 21.8 KDa and p40: 35.8 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP2025
Alternate Names:
NKSF, CLMF
Source:
HEK 293 cells
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
• UV spectroscopy at 280 nm.
• RP-HPLC calibrated against a known standard.
• Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is measured by the dose-dependent mouse primary T cell induced expression of CD25, with Bioactivity Acceptance Criteria ED50 at 20 ng/mL.
AA Sequence:
p35:
MRVIPVSGPA RCLSQSRNLL KTTDDMVKTA REKLKHYSCT AEDIDHEDIT RDQTSTLKTC LPLELHKNES CLATRETSST TRGSCLPPQK TSLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA
p40:
MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| IL-12: Mouse Interleukin-12 | Size: 10 ug | CAT.#: RP2025-10 | Price: $195.00 | |
| IL-12: Mouse Interleukin-12 | Size: 100 ug | CAT.#: RP2025-100 | Price: $1,410.00 | |
| IL-12: Mouse Interleukin-12 | Size: 1000 ug | CAT.#: RP2025-1000 | Price: $8,460.00 |